悬浮培养玫瑰茄细胞的生长行为及动力学方程的建立

The Culturing Process and Kinetic Model Construction of Hibiscus sabdariffa Suspension Culture

摘要: 以培养多年的玫瑰茄(Hibiscus sabdariffa L.)白色细胞株为材料,在以3%蔗糖为碳源,植物生长调节剂为4.5μmol/L 2,4-D(2,4-二氯苯氧乙酸),2.3μmol/LKt(Kinetin,细胞激动素)的悬浮培养条件下,研究了玫瑰茄细胞的生长及泛醌积累动态、培养液pH值的变化以及培养过程中玫瑰茄细胞对碳源、氮源、磷源的消耗动态,并以此数据建立了悬浮培养玫瑰茄细胞的生长、蔗糖消耗、泛醌形成的非结构动力学模型。经验证表明,模型与实际情况有较高的拟合度。

Abstract: A tamed Roselle(Hibiscus sabdariffa L.) callus strain was cultured in Gamborg B₅ liquid medium adding 3% sucrose,4.5μmol/L 2,4-D and 2.3μmol/L Kinetin(Kt). The growth and ubiquinone accumulation of Roselle cell, the change of pH, nutrient assimilation in the medium were studied. Anon structured kinetic model was constructed by using these data. Verification showed that the prediction of cell growth, total sugar assumption and ubiquinone accumulation by these model had a very high coincidence with the experimental results.