Abstract: The uptodate advances of genomic in situ hybridization (GISH) for last ten years are reviewed in the article. These methods initially deriving from animal research have been developed in plant study, such as Multicolor Fluorescence in situ Hybridization (McFISH), Third-strand in situ Hybridization (TISH), Comparative Genomic Hybridization (CGH) and Simultaneous in situ Hybridization. GISHapplied widely in plant can reveal the species origin and evolution and determine the truth of distant hybrid containing foreign chromatin. Also it can research the chromosome behaviour including Bchromosome and explore the genomic mapping and genic function. Finally the applicability and resolving power of GISHabout taxonomic distance, genomic size and the length of chromosome fragment are discussed, as well as the future and the status of GISHin plant.