Abstract: The experiment compares the main factors of cryopreservation of Taxus callus. The results show that with the differences of preculture time, saccharose content of culture medium, cryoprotectants and freezing method, the cell vitality varies remarkably. After Taxus callus was precultured in No.62 medium with 8% saccharose for 6 days, the TTCvalue of Taxus calluses after freezing and storage in LN(liquid nitrogen) was high. The most effective cryoprotectant is the mixture of 10% DMSO and 10% sorbital. The effectiveness of the step-freezing and slow-freezing methods was much better than that of the rapid-freezing method.