双低油菜品系含油量与发育种子中PEPc酶和PAL酶活性的相关分析

Analysis of the Correlations Among the Activities of Phosphoenolpyruvate Carboxylase and Phenylalanine Ammonia-lyase in Developing Seeds with Seed Oil Contents in Different Canola Lines

摘要: 以10个含油量不同的双低油菜品系为材料,测定了发育种子中磷酸烯醇式丙酮酸羧化酶(PEPc)和苯丙氨酸解氨酶(PAL)活性动态变化,以及成熟种子的油脂含量,分析了PEPc和PAL酶活性与含油量之间的关系。结果表明:PEPc酶与含油量之间始终存在不显著正相关,说明PEPc酶活性高低对含油量高低有一定作用,但并不是油菜种子油脂合成过程中的关键酶;PAL酶与含油量之间始终存在不显著的负相关,授粉后32 d,两者之间负相关性最高,但该相关性并不显著。

Abstract: 10 canola lines with different seed oil percentages were used to evaluate dynamics of phosphoenolpyruvate carboxylase(PEPc)and phenylalanine ammonia-lyase(PAL)activities in developing seeds and seed oil content,and to analyze the correlations among the activities of PEPc and PAL in deve-loping seeds with seed oil content.Results are as follows:In seed developing process,no significant positive correlations of seed PEPc activities with oil content was found,indicating that seed PEPc activity didn’t play important role in the lipids accumulation and seed PEPc was not the key enzyme in the lipids accumulation;no significant negative correlations of seed PAL activities with seed oil content was found,the correlations of seed PAL activities with oil content was the highest at 32 days after pollination but not significant at 0.05 level.