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马长艳, 魏锦城, 程光宇, 吴国荣. 菠菜铁型超氧化物歧化酶的纯化及性质[J]. 植物科学学报, 1999, 17(2): 122-126.
引用本文: 马长艳, 魏锦城, 程光宇, 吴国荣. 菠菜铁型超氧化物歧化酶的纯化及性质[J]. 植物科学学报, 1999, 17(2): 122-126.
Ma Changyan, Wei Jincheng, Cheng Guangyu, Wu Guorong. PURIFICATION AND PROPERTIES OF AN IRO CONTAINING SUPEROXIDE DISMUTASE FROM SPINACH LEAVES,SPINACEA OLERACEA[J]. Plant Science Journal, 1999, 17(2): 122-126.
Citation: Ma Changyan, Wei Jincheng, Cheng Guangyu, Wu Guorong. PURIFICATION AND PROPERTIES OF AN IRO CONTAINING SUPEROXIDE DISMUTASE FROM SPINACH LEAVES,SPINACEA OLERACEA[J]. Plant Science Journal, 1999, 17(2): 122-126.

菠菜铁型超氧化物歧化酶的纯化及性质

PURIFICATION AND PROPERTIES OF AN IRO CONTAINING SUPEROXIDE DISMUTASE FROM SPINACH LEAVES,SPINACEA OLERACEA

  • 摘要: 用聚丙烯酰胺梯度凝胶电泳法检测出菠菜SOD同工酶谱带中含3 条Fe-SOD活性带。菠菜叶Fe-SOD粗提液经硫酸铵分部沉淀,DEAE-纤维素-A52和Sephadex G-100 柱层析,纯化出单一的Fe-SOD活性带。纯化酶的分子量为42.6 kD,亚基分子量为21 kD.对金属元素的分析表明,该酶每分子含2.6 个Fe 原子。该酶紫外区最大吸收峰为278 nm,等电点为4.6,氨基酸组成和其它来源的Fe-SOD类似。

     

    Abstract: Iron-superoxide dismutase was found in the crude extract from leaves of Spinacea oleracea after gradient PAGE analysis.The enzyme was isolated and purified to homogeneity by using ammonium sulfate fractionation followed by DEAE-Cellulose-A52 chromatography and Sephadex G-100 gel filtration.The molecular mass of the purified Fe-SOD is about 42.6 kD,and its subunit molecular mass is about 21 kD.Metal analysis reveals that the protein contains about 2.6 gram atoms of iron per molecule.The purified enzyme exhibits one absorption maximum in the ultraviolet at 278 nm with a low shoulder of absorption at 260 nm and 290 nm.Its isoelectric point is 4.6.In the aspects of amino acid composition,the enzyme from spinach is most similar to Fe-SOD from other sources.

     

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