Abstract: Certain entophytic bacteria strains were investigated following isolation from the roots of Cymbidium faberi Rolfe in Hubei province,China.An MS medium+ Cymbidium faberi root extract was used in this study.Twentyseven strains were selected based on the morphology of their colonies.According to 16S rRNA gene sequences analysis and comparison with data from the GenBank,they belonged to 14 species of 8 genera,namely Burkholderia phytofirmans,Pseudomonas jessenii,P.cedrina,P.mohnii,P.fragi,P.koreensi,Bacillus megaterium,B.atrophaeus,B.subtilis,Leifsonia shinshuensis,Variovora paradoxus,Erwinia rhapontici,Duganella zoogloeoides, and Acinetobacter lwoffi. Understanding the relationship between these bacteria and Cymbidium faberi is valuable,particularly in relation to the benefits of these bacteria in the cultivation of Cymbidium faberi Rolfe.