摘要:
以普通荞麦(Fagopyrum esculentum Moench)植株带节茎段为外植体,用正交设计法研究不同激素处理对荞麦腋芽、丛生芽和根的诱导及分化过程的影响,建立了荞麦离体培养快速繁殖技术。极差分析表明,6-BA是诱导荞麦茎段腋芽和根发育的主要因素,TDZ是诱导丛生芽的关键因素。诱导荞麦茎段腋芽发育的最佳培养基为:MS0+6-BA1.0mg/L+NAA 0.1mg/L,腋芽诱导率为67.9%。其中,以第二、三节位的腋芽诱导率较高,达80%以上。诱导荞麦茎段丛生芽发育的最佳培养基为:MS0+6-BA4.0mg/L+TDZ 0.06mg/L,丛芽分化率为166.7%。诱导生根的最佳培养基为:1/2MS0+6-BA1.0mg/L+NAA1.0mg/L,生根率为82.8%。该组织培养技术的建立为荞麦快速繁殖提供了新途径。
Abstract:
The effects of different hormone on axinary buds induction,tufty bud differentiation,and rooting were studied by means of orthogonal design and common buckwheat(Fagopyrum esculentum Moench)stem segment explants with a knot. The results showed that 6-BA is a major factor for induction of axianry buds and roots,and that TDZ is the key to induction of tufty bud.The optimal medium for inducing axillary bud development is MS0+6-BA 1.0 mg/L+NAA 0.1 mg/L,with the rate of axillary bud development of 67.9%.Among them,the second and third knot can produce the rate of 80% or more.The MS0 medium containing 6-BA 4.0 mg/L+TDZ 0.06 mg/L is better for inducing tufty buds with the differentiation rate of 166.7%.The optimum medium of root formation is 1/2 MS0+6-BA 1.0 mg/L+NAA 1.0 mg/L,with the rooting rate of 82.8%.At last,a rapid propagation technology was developed for common buckwheat plants.
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