美洲黑杨(中嘉8号)离体叶片再生研究

详细信息

Study on Plant Regeneration of Excised Leaf from Populus deltoids(I-63×I-69)

Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Central China Normal University, Wuhan 430079, China

摘要: 用中嘉8号杨[Populus deltoids(I-63×I-69)]试管苗叶片作外植体,在添加不同生长素和细胞分裂素浓度配比的1/2(N)MS培养基上,筛选出叶片直接分化不定芽的最适培养基1/2(N)MS+0.2mg/LBA+0.02mg/L NAA+25g/L蔗糖+7g/L琼脂,不定芽分化率为85%。同时发现,一定浓度的KT对生根具有促进作用,最适生根培养基为MS+0.08mg/LKT+0.02mg/L NAA+25g/L蔗糖+8g/L琼脂,生根率为82.2%。
- 美洲黑杨[Populus deltoids(I-63×I-69)] /
- 叶片 /
- 再生 /
- 生长调节物质
Abstract: The leaves from aseptuly plantets of Populus deltoids(I-63×I-69) were cultured as explants on 1/2(N)MS medium containing various combination of auxin and cytokinin.The results showed that the best medium for buds differentiation directly is 1/2(N)MS+0.2 mg/L BA+0.02 mg/L NAA+25 g/L sucrose+7 g/L agar,with the bud differentiation rate of 85%. In the experiments we also found KT has the stimulation to the plants rooting of I-63×I-69,the better rooting medium is MS+0.08 mg/L KT+(0.02 mg/L) NAA+25 g/L sucrose+7 g/L agar,with the rooting rate of 82.2%.
- Populus deltoids /
- Leaf /
- Regeneration /
- Growth regulator