满江红鱼腥藻的相对耐热性
RELATIVE HEAT TOLERANCE OF ANABAENA AZOLLAEMEASURED BY THE CHLOROPHYLL-FLUORESCENCE METHOD
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摘要: 利用加温处理引起藻丝叶绿素a可变荧光强度的变化,以测定满江红鱼腥藻(Anabaena azollae imbricata)的相对耐热性。藻丝在25℃下可变荧光和固定荧光的比值为0.35±0.05;在37℃下(5分钟),相应的比值为0.27;在45℃下比值降至0.11。在50℃下,叶绿素a可变荧光几乎完全消失。较高温度可能引起光合电子传递链成分的构象和功能的变化,减少了光能的利用。结果表明满江红鱼腥藻可以忍受37℃的温度。Abstract: Heat tolerance was determined for Anabaena azollae. The decrease in the variable fluorescence (Fv) yield at 680 nm after heating filaments of A. azollae at 37℃, 45℃and 50℃ each for 5 min. was found as compared with that at 25℃. The ratio of Fv to instantaneous fluorescence (Fo)was 0.35 at 25℃ and the decreases following heating at 37℃and 45℃ were found. And these ratios were 0.27 and 0.11, respectively, As compared with that at 25℃, the relative heat tolerances were 77.2% and 31.5%, respectively. The results showed that the filaments of A. azollae can stand with the temperature stress lower than 37℃ typical observed in the summer.
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