云南小麦高分子量麦谷蛋白亚基基因<I>1Dy12</I><sup>*</sup>的分离

代小华; 房敬业; 李宗瑾; 刘勇; 邹珂; 汪越胜; 常俊丽; 何光源

云南小麦高分子量麦谷蛋白亚基基因1Dy12^*的分离

华中科技大学生命科学与技术学院分子生物物理教育部重点实验室, 武汉 43007

详细信息

通讯作者:
何光源, E-mail:hegy@hust.edu.cn

中图分类号: Q946.1
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出版历程
- 收稿日期: 2008-03-09
- 修回日期: 2008-05-06
- 发布日期: 2008-08-28

Isolation of High Molecular Weight Glutenin Subunit Gene 1Dy12^* from Yunnan Wheat(Triticum aestivum subsp.yunnanense King)

Key Laboratory of Molecular Biophysics, Ministry of Education, Huazhong University of Science and Technology(HUST), Wuhan 430074, China

摘要

摘要: 通过SDS-PAGE分析,从云南小麦中鉴定出一个电泳迁移率比高分子量麦谷蛋白亚基1Dy12稍快的亚基1Dy12^*。利用Glu-Dy位点特异引物对1Dy12^*基因编码区进行了克隆和序列测定。1Dy12^*基因全长为1980bp,编码658个氨基酸。氨基酸序列比较结果表明:与亚基1Dy12相比有3个氨基酸的差异和1个二肽(GQ)的缺失,与亚基1Dy10相比有15个氨基酸的差异、2个六肽(IGQGQQ)的插入以及1个二肽(GQ)的缺失。这表明1Dy12^*亚基是一个新型高分子麦谷蛋白亚基,其对小麦加工品质的影响正在评价中。
- 云南小麦 /
- 高分子量麦谷蛋白亚基 /
- 序列分析
Abstract: A novel y-type high molecular weight glutenin subunit(HMW-GS) possessing a slightly faster mobility than that of subunit 1Dy12 was identified through SDS-PAGE analysis,designated 1Dy12^* in Yunnan wheat(Triticum aestivum subsp.yunnanense King).The coding region of HMW-GS gene 1Dy12^* was isolated and sequenced with Glu-Dy locus specific primers.The complete open reading frame(ORF) of 1Dy12^* was 1980 bp,encoding 658 amino acids.Comparison of amino acid sequences showed there were 3 amino acid differences between subunits 1Dy12^* and 1Dy12 and a deletion of dipeptide(GQ) in subunit 1Dy12^*.Also,there were fifteen amino acid differences between subunits 1Dy12^* and 1Dy10,two hexapeptides(IGQGQQ) insertions and a deletion of dipeptide(GQ) in subunit 1Dy12^*.This showed that subunit 1Dy12^* is a novel HMW-GS,and it is in process of evaluating subunit 1Dy12^* to effect wheat processing quality.
- Yunnan wheat /
- HMW-GS /
- Sequence analysis