Genetic and morphological variation patterns of Macromitrium gymnostomum and their geographical backgrounds
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摘要: 缺齿蓑藓(Macromitrium gymnostomum Sull.&Lesq.)广布于我国东南部,形态变异较大,与近缘种关系模糊。为了正确鉴定缺齿蓑藓形态变异范围,以及形态、遗传和地理因素三者的关系,对缺齿蓑藓11个地理居群106个样本配子体的13个形态性状进行了测定。结果显示,根据形态数据可将106份样本聚类成8个形态组。用13对ISSR引物获得了150个位点,其中148个为多态位点,多态位点百分率为98.67%;Nei’s基因多样性在居群内占62.22%,在居群间占37.78%;居群间基因流(Nm)为0.8235,遗传分化指数(Gst)为0.3778。11个地理居群的遗传距离在0.0873~0.2363之间,平均为0.1508。基于148个多态位点可将106份样本聚成8个遗传组。缺齿蓑藓的形态变异有一定的遗传背景(r=0.159,n=106,P < 0.2),地理因素对形态(r=0.309,n=106,P < 0.01)和遗传(r=0.251,n=106,P < 0.01)分化产生了极显著影响。Abstract: Macromitrium gymnostomum Sull. & Lesq. is widely distributed in southeastern China, with high morphological variation and confusion with allied species. To correctly define the morphological variation range of M. gymnostomum and the relationship among morphological patterns, genetics, and geographical factors, 13 morphological traits based on 106 samples from 11 populations of M. gymnostomum were determined. The 106 samples were preliminarily clustered into eight groups based on the 13 morphological traits. Using 13 pairs of ISSR primers, 150 ISSR bands were amplified, of which 148 (98.67%) were polymorphic. The intra-population genetic diversity accounted for 62.22%, whereas the inter-population value accounted for 37.78%. Gene flow existed among populations, with a Nm index of 0.8235 and a Gst of 0.3778. The genetic distance among the 11 geographical populations varied from 0.0873 to 0.2363, with an average of 0.1508. Based on information from the 148 loci, the 106 samples were clustered into eight groups. The morphological variations of M. gymnostomum were genetically controlled to an extent (r=0.159, n=106, P < 0.2). Distinctive geographical influences on morphological (r=0.309, n=106, P < 0.01) and genetic differentiation (r=0.251, n=106, P < 0.01) were also found.
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[1] 吴鹏程, 贾渝. 中国苔藓志:第5卷[M]. 北京:科学出版社, 2011:86-99. Wu PC, Jia Y. Flora Bryophytorum Sinicorum:Vol. 5[M]. Beijing:Science Press, 2011:86-99.
[2] Potier de la Varde R. Recoltes bryologiques en Afrique Equatoriale Francaise[J]. Ann Cryptog Exot, 1932, 5:53-67.
[3] Brotherus VF. Musci novi Japonici[J]. Öfvers Finska Vetensk.-Soc Förh, 1919-1920, 62A(9):1-55.
[4] Mitten W. On some species of Musci and Hepaticae, additional to the floras of Japan and the coast of China[J]. J Proc Linn Soc Bot, 1864, 8:148-162.
[5] Guo SL, Cao T, Tan BC, Song GY. Taxonomic notes onAsian species of Orthotrichaceae (Bryopsida):Macromitrium with gymnostomous capsules[J]. Gard Bull Singapore, 2007, 58(2):155-178.
[6] 李丹丹, 郭水良, 于晶, 李莎, 曹同. 基于4个叶绿体基因识别蓑藓属(Macromitrium)植物的可行性研究[J]. 植物科学学报, 2013, 31(1):23-33. Li DD, Guo SL, Yu J, Li S, Cao T. Feasibility study on the Identification of genus Macromitrium based on four chloroplast genes[J]. Plant Science Journal, 2013, 31(1):23-33.
[7] Yu J, Guo SL, Ma YH, Cao T. Taxonomic and morphometric comments on Macromitrium tosae Besch. (Orthotrichaceae), with its four new synonyms[J]. Bryologist, 2012, 115(3):388-401.
[8] 汪琛颖, 赵建成. 狭边大叶藓(Rhodobryum ontariense)遗传多样性的ISSR分析[J]. 贵州师范大学学报:自然科学版, 2010, 28(4):33-40. Wang CY, Zhao JC. Genetic diversity in a medicinal plant species:Rhodobryum ontariense(Bryaceae), detected by ISSR[J]. Journal of Guizhou Normal University:Natural Sciences, 2010, 28(4):33-40.
[9] 郭水良, 郑园园, 娄玉霞, 沈蕾. 基于RAPD数据探讨中华蓑藓(Macromitrium cavaleriei Card. & Thér.)形态变异的遗传基础[J]. 植物科学学报, 2011, 29(3):312-318. Guo SL, Zhen YY, Lou YX, Shen L. Genetic background of morphological variation in Macromitrium cavaleriei Card. & Thér. based on RAPD[J]. Plant Science Journal, 2011, 29(3):312-318.
[10] Spagnuolo V, Terracciano S, Cobianchi RC. Taxonomy of the Hypnum cupressiforme complex in Italy based on ITS and trnL sequences and ISSR markers[J]. Trans British Bryolog Society, 2013, 30(4):283-289.
[11] Shaw AJ, Andrus RE, Shaw B. Sphagnum beringiense sp. nov. (Bryophyta) from Arctic Alaska, based on morphological and molecular data[J]. Syst Bot, 2008, 33(3):469-477.
[12] 刘丽, 朱永青, 王幼芳. 鼠尾藓不同居群间形态及RAPD分析[J]. 云南植物研究, 2006, 28(6):570-574. Liu L, Zhu YQ, Wang YF. Morphological and RAPD analyses of different Myuroclada maxiowiczii (Brachy-theciaceae) populations[J]. Acta Botanica Yunnanica, 2006, 28(6):570-574.
[13] 李朝阳, 李菁, 田向荣, 姜业芳, 陈军. 梵净山尖叶拟船叶藓的遗传多样性分析[J]. 云南植物研究, 2008, 30(2):168-172. Li ZY, Li J, Tian XR, Jiang YF, Chen J. Analysis on genetic diversity of Dolichomitriopsis diversiformis (Lembophyllaceae) in Fanjing Mountain[J]. Acta Botanica Yunnanica, 2008, 30(2):168-172.
[14] 李倩影, 曹同, 于晶, 俞鹰浩. 不同种群狭叶小羽藓(Haplocladium angustifolium)重金属含量及遗传多样性[J]. 上海师范大学学报:自然科学版, 2010, 39(2):194-199. Li QY, Cao T, Yu J, Yu YH. Genetic diversity and heavy metal contents of different populations of the moss Haplocladium angustifolium[J]. Journal of Shanghai Normal University:Natural Sciences, 2010, 39(2):194-199.
[15] 张晗, 高永超, 沙伟, 李汝玉, 戴双. 紫萼藓科植物DNA提取及遗传多样性的分析[J]. 山东农业科学, 2006(1):7-9. Zhang H, Gao YC, Sha W, Li RY, Dai S. Study of DNA extraction and genetic diversity in Grimmiaceae[J]. Shandong Agricultural Sciences, 2006(1):7-9.
[16] 戴智慧, 龙骏, 俞雷民, 倪穗. 宁波地区野生换锦花遗传多样性的ISSR分析[J]. 核农学报, 2016, 30(10):1898-1905. Dai ZH, Long J, Yu LM, Ni S. Genetic diversity of wide Lycorissprengeri populations from different habitats of Ningbo based on ISSR analysis[J]. Journal of Nuclear Agricultural Sciences, 2016, 30(10):1898-1905.
[17] 李倩影. 小羽藓属(Haplocladium)植物遗传多样性及其与环境因子关系分析[D]. 上海:上海师范大学, 2010:41-42. Li QY. Genetic biodiversity of moss genus Haplocladium and its relationship with the environmental factors[D]. Shanghai:Shanghai Normal University, 2010:41-42.
[18] 孙鹏雁. 中国曲柄藓属(Campylopus)植物的形态与系统学研究[D]. 上海:上海师范大学, 2015:66. Sun PY. A morphological and phylogenetic study on Campylopus of China[D]. Shanghai:Shanghai Normal University, 2015:66.
[19] 魏青永, 郭水良, 曹同, 于晶. 基于ISSR数据探讨卷叶凤尾藓(Fissidens dubius P. Beauv.)遗传多样性[J]. 植物科学学报, 2016, 34(2):238-245. Wei QY, Guo SL, Cao T, Yu J. Genetic diversity of Fissidens dubius P. Beauv. based on ISSR data[J]. Plant Science Journal, 2016, 34(2):238-245.
[20] 王莹莹. 浙江千岛湖生境片断化对苔藓植物物种及遗传多样性的影响[D]. 上海:华东师范大学, 2011:36. Wang YY. Effects of habitat fragmentation on bryophytes richness and genetic diversity in Thousand-Island Lake region, Zhejiang[D]. Shanghai:East China Normal University, 2011:36. [21] 石蕾. R语言在藓类形态与遗传变异研究中的应用——以大灰藓为例[D]. 上海:上海师范大学, 2015:47. Shi L. Application of R in studies of morphological and genetic variation of plant populations-A case of Hypnum plumaeforme Wilson[D]. Shanghai:Shanghai Normal University, 2015:47.
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