秦岭落叶阔叶林不同空间尺度下土壤微生物特征

李永恒; 李乾玺; 吴君君; 程晓莉

doi:10.11913/PSJ.2095-0837.2020.30335

秦岭落叶阔叶林不同空间尺度下土壤微生物特征

李永恒^1,2,3,
李乾玺³,
吴君君³,
程晓莉^3, ,

1. 西藏大学青藏高原生态与环境研究中心, 拉萨 850000;
2. 西藏大学理学院, 拉萨 850000;
3. 中国科学院武汉植物园, 中国科学院水生植物与流域生态重点实验室, 武汉 430074

基金项目:

国家自然科学基金（31470557，31770563）。

详细信息

作者简介:
李永恒(1993-),男,硕士研究生,研究方向为土壤生态学(E-mail:zoccolo_lee@163.com)。

通讯作者:
程晓莉,E-mail:xlcheng@fudan.edu.cn

中图分类号: Q948
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出版历程
- 收稿日期: 2019-09-03
- 修回日期: 2019-10-24
- 网络出版日期: 2022-10-31
- 发布日期: 2020-06-27

Soil microbial attributes at different spatial scales in deciduous broad-leaved forest in Qinling Mountains

1. Research Center for Ecology and Environment of Qinghai-Tibetan Plateau, Tibet University, Lhasa 850000, China;
2. College of Science, Tibet University, Lhasa 850000, China;
3. Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China

Funds:

This work was supported by grants from the National Natural Science Foundation of China (31470557,31770563).

摘要

摘要: 在秦岭佛坪国家级自然保护区内的固定样地，设置3种不同空间尺度（大：20 m×20 m，中：5 m×5 m，小：1.25 m×1.25 m）的样方，采用野外调查和室内分析的方法，对固定样地土壤微生物特征及其环境因子进行研究，探讨不同空间尺度下土壤微生物特征（磷脂脂肪酸（PLFAs）生物量、土壤微生物群落结构和基础土壤呼吸）对环境因子的响应。结果显示，土壤全磷、溶解性有机碳和溶解性总氮含量在3种空间尺度间有显著差异（P < 0.05）。对微生物PLFAs生物量的研究显示，放线菌生物量在小空间尺度下显著低于大、中空间尺度（P< 0.05），其他微生物PLFAs生物量都没有显著差异。支链/单不饱和PLFAs的比值在大空间尺度下显著高于中、小空间尺度（P< 0.05），其他微生物群落结构特征都没有显著差异。基础土壤呼吸在大空间尺度和小空间尺度间的差异显著（P < 0.05）；单位PLFAs的基础土壤呼吸则表现出中、小空间尺度显著高于大空间尺度（P < 0.05）。相关性分析和冗余分析表明，不同空间尺度下，环境因子对微生物特征的解释度也存在明显差异。大空间尺度下，解释度最高的环境因子是土壤有机碳、含水率和溶解性氮；中空间尺度下，解释度最高的是含水率、土壤有机氮和溶解性有机碳；小空间尺度下，解释度最高的是pH、全磷含量和土壤有机氮。本研究结果表明由于尺度效应的存在，导致随着取样尺度的缩小，环境因子的交互作用增强和空间自相关程度增大，从而表现出在不同空间尺度下环境因子对土壤微生物的影响存在差异。
- 尺度效应 /
- 微生物PLFAs /
- 土壤微生物群落结构 /
- 基础土壤呼吸 /
- 秦岭
Abstract: We established three different spatial-scale fixed plots in Foping National Nature Reserve in the Qinling Mountains. Based on field investigation and indoor analysis, we investigated soil microbial characteristics and environmental factors in order to explore the responses of microbial phospholipid fatty acid (PLFA) biomass, microbial structure, and respiration to environmental factors. Results showed that microbial PLFA biomass did not significantly change among the three sampling scales, except actinomycete biomass was higher at the small scale compared with the other scales (P < 0.05). Similarly, microbial community structure was not significantly different, except the branched/monounsaturated PLFA ratio was significantly higher at the large scale than the small and mid-scales (P < 0.05). Pearson correlation analysis indicated that there were significant differences in the correlation between microbial characteristics and environmental factors at different spatial scales. Moreover, redundancy analysis (RDA) showed that the interpretation of microbial attributes by environmental factors differed at different spatial scales. Namely, the most important controllers of microbial attributes were soil organic carbon, water content, and dissolved nitrogen at the large scale; water content, soil organic nitrogen, and dissolved organic carbon at the mid-scale; and soil pH, total phosphorus content, and soil organic nitrogen at the small scale. Thus, our results showed that the interactions among environmental factors and degree of spatial autocorrelation increased at the small scale due to the existence of the scale effect, indicating that environmental factors had different effects on soil microorganisms at different spatial scales.
- Scale effect /
- Microbial PLFAs /
- Soil microbial community structure /
- Basic soil respiration /
- Qinling Mountains

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