Esterase Isozymes of Young Leaves at Three-leaf Stage of Genus Fagopyrum Plants
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摘要: 用聚丙烯酰胺凝胶电泳技术对荞麦属(Fagopyrum Mill)8个种(含大粒组7个种和小粒组1个种)29份栽培及野生荞麦植株三叶期幼叶的酯酶同工酶进行了研究。结果发现:酯酶同工酶酶带共31条,不同物种的酶带数4~9条,其中甜荞有8条带,而苦荞为9条。酶带分析及聚类分析表明:大粒组荞麦种的谱带与细野荞(F.gracili-pes)等小粒组荞麦种间差异极大,甜荞(F.esculentum)和苦荞(F.tataricum)酶带分别与大野荞(F.megaspartanium)和毛野荞(F.pilus)相似,并分别与大野荞和毛野荞聚类最近,提示大野荞和毛野荞可能分别是甜荞和苦荞的祖先种。Abstract: The esterase isozymes of twenty-nine accessions of cultivated and wild buckwheat belonging to eight species of genus Fagopyrum were studied by means of polyacrylamide gel eletrophoresis(PAGE).The results showed that there are a total of thirty-one bands of esterase isozymes,with a range from four bands to nine bands among different buckwheat species.F.esculentum and F.tataricum respectively have a total of eight bands and nine bands among different accessions.The results of the zymograph analysis and the clustering showed that there are much great difference of zymographs between the big-achene group and the small-achene group and that F.megaspartanium and F.pilus are close in clustering tree to F.esculentum and F.tataricum,respectively,supporting Chen’s hypothesis that F.megaspartanium and F.pilus may be the ancestors of F.esculentum and F.tataricum,respectively.
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Keywords:
- Common buckwheat /
- Tartary buckwheat /
- Wild buckwheat /
- Esterase isozyme /
- Phylogeny
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