Effects on Ammonia Volatilization from Shoot of Rice by Inhibitor
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摘要: 在营养液培养条件下,对两个不同氮效率基因型水稻品种扬稻6号和武育粳3号采用光呼吸抑制剂异烟肼(INH)和谷氨酰胺合成酶(GS)抑制剂蛋氨酸亚砜亚胺(MSO)处理,研究其对水稻光合速率、光呼吸速率、GS酶活性及冠层的NH3挥发速率的影响。结果发现:(1)MSO导致剑叶光合速率下降,光呼吸速率升高;INH导致光呼吸速率显著下降,同时一定程度上引起光合速率降低。(2)MSO处理显著降低了GS酶活性,相应地引起NH3挥发速率增加;INH在一定程度上导致NH3挥发速率降低。(3)扬稻6号NH3挥发速率比武育粳3号低的生理原因是光呼吸速率较低和GS酶活性较高。Abstract: Pot experiments were conducted using Yoshida culture solution with two rice varieties(Wuyujing-3 and Yangdao-6) to study the effects of glutamine synthetase inhibitor methionine sulfoximine(MSO) and photorespiration inhibitor isonicotinyl hydrazine(INH) on the ammonia volatilization rate(AVR),photorespiration rate(Pr) and glutamine synthetase(GS) activity in rice.The results showed that:(1)MSO decreased the Pn,but increased the Pr in the flag leaf;INH decreased the Pr significantly and Pn slightly.(2)MSO decreased GS activity,which promoted an increase in AVR in rice canopy.INH showed a slightly positive effect on the decrease in AVR.(3)Based on the comparison between the two tested varieties,the higher AVR variety(Yangdao-6) showed greater GS activity and lower Pr than those of the lower AVR variety(Wuyujing-3).
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Keywords:
- Rice /
- Photorespiration rate /
- Glutamine synthetase /
- Ammonia volatilization rate
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