花生愈伤组织的诱导和再生体系的建立

Callus Induction and Establishment of Efficient Regeneration System of Arachis hypogaea L.

摘要: 以花生下胚轴为外植体,MS和1/2 MS为基本培养基,用不同浓度的激素及琼脂对愈伤组织诱导及分化的影响进行了试验,确定MS+6-BA 3.0 mg·L^-1+NAA 0.4 mg·L^-1+0.7%琼脂为诱导愈伤的最佳培养基。提高琼脂浓度可有效抑制玻璃化和褐化的发生,且琼脂浓度越大,愈伤组织越易分化出丛生芽。丛生芽在1/2 MS+NAA1.0 mg·L^-1+0.7%琼脂培养基上生根率最高,达95%以上。

Abstract: The tissue culture of peanut(Arachis hypogaea L.) was studied.The hypocotyls were used as explants on basal MS or 1/2 MS medium supplemented with different hormone combinations and agar.The result showed that the best medium for the inducement of callus was MS+6-BA 3.0 mg·L^-1+NAA 0.4 mg·L^-1+0.7% agar.Increasing of concentration of agar could inhibit vetrification and browning,and make callus easier to differentiate buds.The medium with 1/2 MS+NAA 1.0 mg·L^-1+0.7% agar was favorable to the growth of roots and the rate of rooting was more than 95%.