具晶泡绿藻(<i>Vacuoliviride crystalliferum</i>)——分离自特殊生境的真眼点藻纲中国新纪录属种

张琪; 郑凌凌; 李天丽; 宋立荣

doi:10.11913/PSJ.2095-0837.2018.40518

具晶泡绿藻(Vacuoliviride crystalliferum)——分离自特殊生境的真眼点藻纲中国新纪录属种

1. 中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 武汉 430072;
2. 中国科学院水生生物研究所, 中国科学院藻类生物学重点实验室, 武汉 430072

基金项目:

现代农业产业技术体系专项资金资助（CARS-50）；中国科学院战略生物资源服务网络计划生物遗传资源库保藏能力建设专项（ZSSB-006）。

详细信息

作者简介:
张琪(1985-),男,博士,主要从事淡水藻类分类及系统发育研究(E-mail:zhangq@ihb.ac.cn)。

通讯作者:
宋立荣,E-mail:lrsong@ihb.ac.cn

中图分类号: Q949.25
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出版历程
- 收稿日期: 2017-11-02
- 网络出版日期: 2022-10-31
- 发布日期: 2018-08-27

Vacuoliviride crystalliferum Nakayama, Nakamura, Yokoyama, Shiratori, Inouye & Ishida, a new record of the genus and species of Eustigmatophyceae algae from a special habitat in China

1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
2. Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

Funds:

This work was supported by grants from the China Agriculture Research System (CARS-50) and Biological Resources Service Network Initiative, Chinese Academy of Sciences (ZSSB-006).

摘要

摘要: 从南京产白乳胶样品中分离和培养得到1株藻种，编号为FACHB-2245，对其进行形态观察和SSU基因序列分析。结果显示，该藻株为单细胞，球状至椭球状，细胞壁平滑。细胞具有1个大液泡、1个红色球体和1个透明晶状结构以及许多折光颗粒。叶绿体周生，1至多个，具膨大的蛋白核。结合形态和分子系统发育分析，确定这株藻种为真眼点藻纲的具晶泡绿藻（Vacuoliviride crystalliferum Nakayama，Nakamura，Yokoyama，Shiratori，Inouye&Ishida）。该属种在我国是首次报道，生活于白乳胶这一特殊生境。基于SSU序列的系统发育研究表明，具晶泡绿藻藻株与真眼点藻纲中的角绿藻属（Goniochloris Geitler）、粗盘藻属（Trachydiscus Ettl）和假十字趾藻属（Pseudostaurastrum Chodat）的亲缘关系密切，位于这些类群的基部。
- 真眼点藻纲 /
- 具晶泡绿藻 /
- 特殊生境 /
- 新纪录 /
- 中国
Abstract: An algal strain sampled from Nanjing, Jiangsu Province, China, was isolated via vinyl acetate-ethylene emulsion and deposited at the Freshwater Algae Culture Collection at the Institute of Hydrobiology (FACHB-2245). The solitary spherical or ellipsoidal cells had a large vacuole, reddish globule, crystalline structure, and some refractile granules. The cells possessed one to several greenish parietal chloroplasts with a bulging pyrenoid. Based on morphological and molecular phylogenetic evidence, the strain was identified as eustigmatophycean species Vacuoliviride crystalliferum Nakayama, Nakamura, Yokoyama, Shiratori, Inouye & Ishida. The species is considered as a new record of the genus and species in China. Phylogenetic analyses demonstrated that the strains of V. crystalliferum were clustered with Goniochloris Geitler, Trachydiscus Ettl, and Pseudostaurastrum Chodat.
- Eustigmatophyceae /
- Vacuoliviride crystalliferum /
- Special habitat /
- New record /
- China

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参考文献(25)

[1]	Hibberd DJ, Leedale GF. Eustigmatophyceae-a new algal class with unique organization of the motile cell[J]. Nature, 1970, 225:758-760.
[2]	Hibberd DJ, Leedale GF. A new algal class:the eustigmatophyceae[J]. Taxon, 1971, 20(4):523-526.
[3]	高保燕, 张成武, 万凌琳, 李爱芬. 真眼点藻纲的系统分类、生物学特性及应用研究[J]. 水生生物学报, 2014, 38(5):945-956. Gao BY, Zhang CW, Wan LL, Li AF. Systematics, biological characteristics and potential application of Eustigmatophyceae[J]. Acta Hydrobiologica Sinica, 2014, 38(5):945-956.
[4]	Ott DW, Oldham-Ott GK. Eustigmatophyceae, Raphidophyceae, and Tribophyceae[M]//Wehr JD, Sheath RG, eds. Freshwater Algae of North America Ecology and Classification. Massachusetts:Academic Press, 2003:421-427.
[5]	Hibberd DJ, Leedale GF. Observation on the cytology and ultrastructure of the new algal class, Eustigmatophyceae[J]. Ann Bot, 1972, 36(1):49-71.
[6]	Whittle SJ, Casselton PJ. The chloroplast pigments of the algal classes Eustigmatophyceae and Xanthophyceae.Ⅰ. Eustigmatophyceae[J]. Br Phycol J, 1975, 10(2):179-191.
[7]	Fawley KP, Eliáš M, Fawley MW. The diversity and phylogeny of the commercially important algal class Eustigmatophyceae, including the new clade Goniochloridales[J]. J Appl Phycol, 2014, 26:1773-1782.
[8]	Moazami N, Ashori A, Ranjbar R, Tangestani M, Eghtesadi R, Nejad AS. Large-scale biodiesel production using microalgae biomass of Nannochloropsis[J]. Biomass Bioenergy, 2012, 39:449-453.
[9]	van Wagenen J, Miller TW, Hobbs S, Hook P, Crowe B, Huesemann M. Effects of light and temperature on fatty acid production in Nannochloropsis salina[J]. Energies, 2012, 5(3):731-740.
[10]	Prior SE, Fawley MW, Fawley KP. DNA sequence analysis of freshwater Eustigmatophyceae, a potential source of essential fatty acids[J]. J Ark Acad Sci, 2010, 63:139-144.
[11]	Stanier RY, Kunisawa R, Mandel M. Purification and properties of unicellular blue-green algae[J]. Bacteriol Rev, 1971, 35(2):171-205.
[12]	Hillis DM, Moritz C, Mable BK. Molecular Systematics[M]. 2nd ed. Sunderland, MA:Sinauer Associates, 1996:665.
[13]	Katana A, Kwiatowski J, Spalik K, Zakryś B, Szalacha E, Szymanska H. Phylogenetic position of Koliella (Chlorophyta) as inferred from nuclear and chloroplast small subunit rDNA[J]. J Phycol, 2001, 37(3):443-451.
[14]	Thompson JD, Gibson TJ, Plewniak F. The CLUSTAL_X windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools[J]. Nucleic Acids Res, 1997, 25(24):4876-4882.
[15]	Hall TA. BioEdit:a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT[J]. Nucleic Acids Symp Ser, 1999, 41, 95-98.
[16]	Stamatakis A. RAxML-VI-HPC:maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models[J]. Bioinformatics, 2006, 22(21):2688-2690.
[17]	Huelsenbeck J, Ronquist F. MrBayes:bayesian inference of phylogenetic trees[J]. Bioinformatics, 2001, 17:754-755.
[18]	Darriba D, Taboada GL, Doallo R, Posada D. jModelTest 2:more models, new heuristics and parallel computing[J]. Nat Methods, 2012, 9(8):772.
[19]	Nakayama T, Nakamura A, Yokoyama A, Shiratori T, Inouye I, Ishida K. Taxonomic study of a new eustigmatophycean alga, Vacuoliviride crystalliferum gen. et sp. nov[J]. J Plant Res, 2015, 128:249-257.
[20]	Hibberd DJ. Observations on the cytology and ultrastructure of Chlorobotrys regularis (West) Bohlin with special reference to its position in the Eustigmatophyceae[J]. Br Phycol J, 1974, 9:37-46.
[21]	Neustupa J, Němcová Y. Morphological and taxonomic study for three terrestrial eustigmatophycean species[J]. Nova Hedwigia, 2001, 123:373-386.
[22]	Preisig HR, Wilhelm C. Ultrastructure, pigments and taxonomy of Botryochloropsis similis gen. et sp. nov (Eustigmatophyceae)[J]. Phycologia, 1989, 28:61-69.
[23]	Přibyl P, Eliáš M, Cepák V, Lukavský J, Kaštánek P. Zoosporogenesis, morphology, ultrastructure, pigment composition, and phylogenetic position of Trachydiscus minutus (Eustigmatophyceae, Heterokontophyta)[J]. J Phycol, 2012, 48:231-242.
[24]	Dashiel C, Bailey J. New observations on the biology of eustigmatophytes, with a description of Microtalis gen. nov.[J]. J Phycol, 2009, 45:8.
[25]	Fukuda S, Iwamoto K, Atsumi M, Yokoyama A, Nakayama T, Ishida K, Inouye I, Shiraiwa Y. Global searches for microalgae and aquatic plants that can eliminate radioactive cesium, iodine and strontium from the radio-polluted aquatic environment:a bioremediation strategy[J]. J Plant Res, 2014, 127:79-89.