Physiological responses of Erythrophleum fordii seedlings under SNP-AlCl3 interaction
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摘要: 以格木（Erythrophleum fordii Oliv.）幼苗为材料，采用双因素完全随机设计实验方法，测定不同处理幼苗的光合色素和可溶性糖等生理指标，研究格木幼苗对硝普钠（SNP）-氯化铝（AlCl3）互作的生理响应。结果显示，格木幼苗叶片中叶绿素a、叶绿素b和类胡萝卜素含量均在处理4（0.2 mmol/L AlCl3、0.1 mmol/L SNP）时最高，在处理9（0.8 mmol/L AlCl3、0 mmol/L SNP）时含量最低，而叶片中丙二醛（MDA）、游离脯氨酸含量则相反；叶片可溶性糖、可溶性蛋白含量在处理4时最高，在处理9时最低；处理10（0.8 mmol/L AlCl3、0.1 mmol/L SNP）的超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性最高。施加SNP后，格木幼苗叶片中的叶绿素a、叶绿素b、类胡萝卜素、可溶性糖、可溶性蛋白含量及SOD、POD、CAT活性均显著高于未施加SNP处理。相关性分析表明，叶绿素a、类胡萝卜素、总叶绿素和可溶性蛋白含量等指标间均呈极显著正相关（P < 0.01）。本研究结果得出，低浓度AlCl3（0.2 mmol/L）胁迫可促进格木幼苗的生长，添加外源SNP对高浓度AlCl3（0.8 mmol/L）胁迫格木幼苗产生的毒害具有一定的缓解作用，可在格木幼苗的培育及抗性研究中推广应用。Abstract: Erythrophleum fordii Oliv. seedlings were used as experimental materials to determine the physiological responses, based on photosynthetic pigment and soluble sugar indices, of differently treated seedlings to SNP-AlCl3 interaction using two-factor completely randomized experiments. Results showed that chlorophylla, chlorophyllb, and carotenoid contents in leaves were the highest under Treatment 4 and the lowest under Treatment 9. However, MDA and free proline content in leaves demonstrated the opposite pattern. Soluble sugar and soluble protein content in leaves was the highest under Treatment 4 and the lowest under Treatment 9. Furthermore, SOD, POD, and CAT showed highest activity under Treatment 10 (0.8 mmol/L AlCl3 and 0.1 mmol/L SNP). After the addition of SNP, the contents of chlorophylla, chlorophyllb, carotenoid, soluble sugar, and soluble protein in leaves of E. fordii seedlings and the activities of SOD, POD, and CAT were significantly higher than when SNP was not added. Based on correlation analysis, we found a significant positive correlation between total content of chlorophylla, carotenoid, chlorophyll, and soluble protein (P < 0.01). Our research suggests that low-concentration AlCl3 (0.2 mmol/L) stress can facilitate E. fordii seedlings growth and exogenous addition of SNP can relieve the toxic effects of high-concentration AlCl3 (0.8 mmol/L) stress to E. fordii seedlings. Thus, these results can be applied for cultivation and resistance research of E. fordii seedlings.
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