Identification of bHLH transcription factors in moso bamboo (Phyllostachys edulis) and their expression analysis under drought and salt stress
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摘要: 以毛竹（Phyllostachys edulis（Carr.）Lehaie）为材料，利用生物信息学方法，在基因组水平上对其bHLH基因家族成员进行鉴定和分析，并对不同组织中该基因的表达模式以及部分基因在干旱和高盐胁迫条件下的表达情况进行研究。结果显示：在毛竹中共鉴定出153个具有完整保守结构域的bHLH基因家族成员（PebHLH001~PebHLH153），这些基因内含子数量为0~14，其中137个基因的启动子均含有与干旱、盐胁迫相关的顺式作用元件；PebHLHs编码的蛋白长度为134~1401 aa；bHLH家族成员的系统进化分析结果表明，153个PebHLHs可被分为17个亚类，其中C亚类的成员数量最多，为42个；基于转录组数据的表达谱分析结果发现，有151个PebHLHs在毛竹不同组织和不同生长发育时期有不同程度的表达量；实时荧光定量PCR实验结果显示，在干旱和盐胁迫处理后，分别有14和13个PebHLHs基因的表达量上调，分别有2和3个表达量下调，但表达模式存在一定差异，说明他们在应答干旱和盐胁迫过程中可能发挥不同的作用。Abstract: The transcription factors of the bHLH family have important regulatory effects on plant growth and development. Here, Phyllostachys edulis (Carr.) Lehaie was used as experimental material to understand the characteristics of bHLH family members in bamboo and explore their expression patterns under adverse conditions. Bioinformatics were used to identify and systematically analyze the bHLH family members at the genomic level, and their expression patterns in different tissues and expression of several bHLH genes under high salt and drought stress conditions were further analyzed. Our results identified 153 bHLH transcription factor genes (PebHLH001-PebHLH153) with complete conserved domains in Ph. edulis. The number of introns in PebHLHs ranged from 0 to 14. The drought and salt stress related cis-acting elements were found in the promoters of 137 PebHLHs. The length of the proteins encoded by PebHLHs ranged from 134 aa (PebHLH005) to 1401 aa (PebHLH083) with molecular weights of 13.4 to 152.6 kD, respectively. Based on phylogenetic analysis of bHLHs in Ph. edulis and Oryza sativa L., 153 PebHLHs were clustered into 17 subgroups, with the C subgroup containing the highest number (42). According to expression profile analysis of transcriptome data, 151 PebHLHs exhibited different expression levels in different tissues and growth stages of Ph. edulis. Real-time quantitative polymerase chain reaction (PCR) showed that 14 PebHLHs were up-regulated and two PebHLHs were down-regulated after drought stress, whereas 13 were up-regulated and three were down-regulated after salt stress. However, there were some differences in the expression patterns of 16 PebHLHs, indicating that they may play different roles in response to drought and salt stress.
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Keywords:
- Phyllostachys edulis /
- bHLH gene family /
- Gene identification /
- Stress /
- Expression analysis
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