香樟全基因组<i>WRKY</i>基因家族的鉴定与分析

倪辉; 孙维红; 丁乐; 曾伟伟; 邹双全

doi:10.11913/PSJ.2095-0837.2022.40513

香樟全基因组WRKY基因家族的鉴定与分析

倪辉^1,2,
孙维红^1,2,
丁乐^1,2,
曾伟伟^1,2,
邹双全^1,2, ,

1. 福建农林大学林学院, 福州 350002;
2. 自然生物资源保育利用福建省高校工程研究中心, 福州 350002

基金项目:

中央财政林业科技推广示范项目(闽[2020]TG07号)

福建省林业科技项目(2021FKJ19)。

详细信息

作者简介:
倪辉(1995-)男,硕士研究生,研究方向为药用植物栽培与利用(E-mail:1536195040@qq.com)。

通讯作者:
邹双全,E-mail:zou@fafu.edu.cn

中图分类号: Q943.2
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出版历程
- 收稿日期: 2022-06-07
- 修回日期: 2022-06-29
- 网络出版日期: 2022-10-31
- 发布日期: 2022-08-27

Identification and analysis of the WRKY gene family in whole genome of Cinnamomum camphora(L.) Presl.

Ni Hui^1,2,
Sun Wei-Hong^1,2,
Ding Le^1,2,
Zeng Wei-Wei^1,2,
Zou Shuang-Quan^1,2, ,

1. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2. Natural Biological Resources Conservation and Utilization, Fujian University Engineering Research Center, Fuzhou 350002, China

Funds:

This work was supported by grants from the Central Finance Forestry Science and Technology Promotion Demonstration Project (Min[2020]TG07) and Fujian Forestry Science and Technology Project (2021FKJ19).

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摘要

摘要: WRKY转录因子基因家族是植物特有的一类基因，在植物次生代谢、生物和非生物胁迫中起着重要的调节作用。本研究通过生物信息学方法，在香樟(Cinnamomum camphora (L.) Presl.)全基因组中鉴定了60个WRKY基因(CcWRKY)，并将其分为Group Ⅰ~ Ⅲ，其中，Group Ⅰ和Ⅲ的成员发生了收缩现象；片段复制是CcWRKY基因扩张的主要驱动力；Group Ⅰ有完整的WRKY结构域和锌指基序，但Group Ⅱ、Ⅲ存在结构域和锌指基序的丢失和变异现象；CcWRKY基因的启动子区域具有激素类和胁迫类响应顺式作用元件；基因表达分析结果显示，在贫瘠环境(未施肥)中大多数CcWRKY基因在香樟各个组织中高表达，而环境适宜(施肥)条件下，基因表达量降低。
- 香樟 /
- WRKY基因 /
- 转录因子 /
- 生物信息学 /
- 表达谱
Abstract: The WRKY transcription factor gene family is a class of plant-specific genes that play important regulatory roles in plant secondary metabolism and biotic and abiotic stresses. Through bioinformatic analysis, 60 WRKY genes were identified in the whole genome of Cinnamomum camphora (L.) Presl., which were divided into Groups Ⅰ,Ⅱ, and Ⅲ. Genes in Group Ⅰ and Group Ⅲ were contracted, while segment duplication was the main cause of CcWRKY gene expansion. The C terminus and N terminus of Group Ⅰ contained a complete WRKY domain and zinc finger motif, however there is loss and variation of the domain and zinc finger motif in members of Group Ⅱ and Group Ⅲ. There were many hormone- and stress-responsive cis-acting elements on the promoter of CcWRKYs, such as ABA, MeJA, SA, drought induction, and anaerobic-related stress response. Expression analysis showed that most CcWRKY genes were highly expressed in various tissues in barren environment (without fertilization), while under suitable environmental (fertilization) conditions, the level of gene expression decreased. This study analyzed the expression patterns of CcWRKY genes in response to abiotic stress and provided a theoretical basis for the subsequent functional verification of the C. camphora WRKY gene.
- Cinnamomum camphora /
- WRKY /
- Transcription factor /
- Bioinformatics /
- Expression profile

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