中国辣椒热胁迫转录因子的全基因组鉴定及热胁迫响应的初步分析

高崇伦; 黄家权; 成善汉; 汪志伟; 尹黎燕

doi:10.11913/PSJ.2095-0837.2020.20249

中国辣椒热胁迫转录因子的全基因组鉴定及热胁迫响应的初步分析

高崇伦^1,2,
黄家权²,
成善汉³,
汪志伟^3, ,,
尹黎燕^1,2, ,

1. 海南大学生命科学与药学院, 热带生物资源教育部重点实验室, 海口 570228;
2. 海南大学热带作物学院, 海南省热带生物资源可持续利用重点实验室, 海口 570228;
3. 海南大学园艺学院, 海南省热带园艺作物品质调控重点实验室, 海口, 570228

基金项目:

国家重点研发计划（2018YFD1000800）；国家自然科学基金项目（31760578）；海南大学高层次人才启动基金（KYQD1656）。

详细信息

作者简介:
高崇伦(1995-),女,硕士研究生,研究方向为植物发育生物学(E-mail:gaochonglun_37@163.com)。

通讯作者:
汪志伟,E-mail:wangzhiwei@hainu.edu.cn

尹黎燕,E-mail:lyyin@163.com

中图分类号: Q943.2
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- 文章访问数: 775
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出版历程
- 收稿日期: 2019-07-31
- 修回日期: 2019-09-02
- 网络出版日期: 2022-10-31
- 发布日期: 2020-04-27

Genome-wide identification of heat stress transcription factors and preliminary analysis of heat stress responses in Capsicum chinense Jacq.

1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, China;
2. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan University, Haikou 570228, China;
3. Hainan Key Laboratory for Quality Control of Tropical Horticultural Crops, College of Horticulture, Hainan University, Haikou 570228, China

Funds:

This work was supported by grants from the National Key Research and Development Program of China (2018YFD1000800), National Natural Science Foundation of China (31760578), and Startup Funding from Hainan University (KYQD1656).

摘要

摘要: 采用生物信息学方法，从中国辣椒（Capsicum chinense Jacq.）全基因组序列中鉴定得到28个热胁迫转录因子（HSF）候选基因，并对这些候选基因的染色体分布、基因结构及编码蛋白的3D结构特征进行了分析。结果显示：28个候选基因的编码蛋白长度为128 ～ 526 aa；系统发育分析结果表明，HSF可分为A、B、C 3个亚家族。进一步对热胁迫处理后的中国辣椒种质进行转录组分析，共检测到27个HSF转录本，与对照组相比，实验组中有25个基因对热胁迫有不同程度的响应。
- 中国辣椒 /
- 热胁迫转录因子 /
- 基因家族
Abstract: Based on bioinformatics, 28 HSF candidate genes were identified in the whole genome sequence of Capsicum chinense Jacq. The chromosome distribution, gene structure, and 3D characteristics of these candidate genes were analyzed. Results showed that the protein length of the 28 candidate genes ranged from 128 to 526 aa. Phylogenetic analysis showed that HSF could be divided into A, B, and C subfamilies. In total, 27 HSF transcripts were detected by RNA-seq. Compared with the control group, 25 genes in the experimental group exhibited different responses under heat stress.
- Capsicum chinense /
- Heat stress transcription factor /
- Gene family

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