青藏高原4种无心菜属植物的细胞学研究

徐波; 陈光富; 李志敏

doi:10.11913/PSJ.2095-0837.2020.60723

青藏高原4种无心菜属植物的细胞学研究

徐波¹,
陈光富^2, ,,
李志敏^3, ,

1. 西南林业大学林学院, 昆明 650224;
2. 丽江师范高等专科学校应用技术学院, 云南丽江 674199;
3. 云南师范大学生命科学学院, 昆明 650500

基金项目:

国家自然科学基金项目（31960046，31460047）；丽江师范高等专科学校实习实训基地建设项目（XJ03201801）。

详细信息

作者简介:
徐波(1983-),男,博士,讲师,研究方向为高山植物多样性及分类(E-mail:alpine_flora@163.com)。

通讯作者:
陈光富,E-mail:chgf178@163.com

李志敏,E-mail:lizhimin_vip@163.com

中图分类号: Q942
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出版历程
- 收稿日期: 2020-03-22
- 修回日期: 2020-04-28
- 网络出版日期: 2022-10-31
- 发布日期: 2020-12-27

Cytological study on four species of Arenaria from Qinghai-Tibet Plateau

1. Forestry College, Southwest Forestry University, Kunming 650224, China;
2. Applied Technology College, Lijiang Normal College, Lijiang, Yunnan 674199, China;
3. School of Life Science, Yunnan Normal University, Kunming 650500, China

Funds:

This work was supported by grants from the National Natural Science Foundation of China (31460047, 31960046) and Lijiang Normal College Internship Training Base Construction Project (XJ03201801).

摘要

摘要: 以四川康定和稻城两个居群的无心菜属（Arenaria）雪灵芝亚属（A. subgen.Eremogoneastrum）的雪灵芝（A.brevipetala Y.W.Tsui et L.H.Zhou）、西藏八宿业拉山和安久拉山两个居群的八宿雪灵芝（A.baxoiensis L.H.Zhou）、西藏当雄和工布江达两个居群的瘦叶雪灵芝（A.ischnophylla Williams）以及西藏拉萨居群的藓状雪灵芝（A.bryophylla Fernald）为研究材料，采用植物根尖常规压片法，对它们的细胞分类学特征进行研究。结果显示：7个居群中，4种植物的染色体数目均为2n=22，为二倍体，核型公式为2n=2x=22=22 m，核型不对称性属于Stebbins's-1A型，AI值在1.75~0.32范围内变化。结合无心菜属已有细胞学资料，推断雪灵芝亚属核型特征原始，染色体数目及倍性稳定。本研究中4种植物的染色体数目和核型资料均为首次报道，补充了青藏高原无心菜属的细胞学资料。
- 无心菜属 /
- 雪灵芝亚属 /
- 细胞学 /
- 青藏高原
Abstract: To determine the cytotaxonomic characteristics of Arenaria in China, A. subgen. Eremogoneastrum A. brevipetala Y. W. Tsui et L. H. Zhou (two populations from Kangding and Daocheng in Sichuan), A. baxoiensis L. H. Zhou (two populations from Yela and Anjula mountains, Baxoi, Tibet), A. ischnophylla Williams (two populations from Damxung and Gongbo'gyamda in Tibet), and A. bryophylla Fernald (Lhasa, Tibet) were studied. Root tips were used in combination with KaryoType 2.0 analysis for karyotype research. Results showed that the chromosome number in all seven populations of the four species was 2n = 22, i.e., diploid evolution. The karyotype formula was 2n=2x=22=22 m and karyotype asymmetry belonged to Stebbins's-1A type, with an AI value in the range of 1.75 - 0.32. Thus, combined with existing cytological data for Arenaria, the karyotype of A. subgen. Eremogoneastrum is primitive, and its chromosome number (ploidy) is stable. The chromosome number and karyotype data of the four studied species are reported here for the first time. These results will supplement current cytological information for Arenaria in the Qinghai-Tibet Plateau and provide insight into the taxonomy, phylogeny, and floristic geography of Arenaria and Caryophyllaceae.
- Arenaria /
- Arenaria subgen. Eremogoneastrum /
- Cytology /
- Qinghai-Tibet Plateau

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参考文献(34)

[1]	张镱锂, 李炳元, 郑度. 论青藏高原范围与面积[J]. 地理研究, 2002, 21(1):1-8. Zhang YL, Li BY, Zheng D. A discussion on the boundary and area of the Tibetan Plateau in China[J]. Geographical Research, 2002, 21(1):1-8.
[2]	吴征镒, 王荷生. 中国自然地理-植物地理(上册)[M]. 北京:科学出版社, 1983.
[3]	Myers N, Mittermeier RA, Mittermeier CG, Fonseca GABD, Kent J. Biodiversity hotspots for conservation prio-rities[J]. Nature, 2000, 403(6772):853-858.
[4]	Wen J, Zhang JQ, Nie ZL, Zhong Y, Sun H. Evolutionary diversifications of plants on the Qinghai-Tibetan Plateau[J]. Front Genet, 2014, 5:4.
[5]	孙文光, 孙航, 李志敏. 染色体数据的挖掘及其在植物多样性进化研究中的利用[J]. 植物科学学报, 2019, 37(2):260-269. Sun WG, Sun H, Li ZM. Chromosome date mining and its application in plant diversity research[J]. Plant Science Journal, 2019, 37(2):260-269.
[6]	中国科学院中国植物志编辑委员会. 中国植物志:第26卷[M]. 北京:科学出版社, 1996:159-195.
[7]	周立华. 论无心菜属的地理分布[J]. 植物分类学报, 1996, 34(3):229-241. Zhou LH. On the geographical distribution of Arenaria L.[J]. Acta Phytotaxonomica Sinica, 1996, 34(3):229-241.
[8]	Li AR, Ban Q. Arenaria dawuensis, a new species of the Caryophyllaceae from Sichuan, China[J]. Acta Phytota-xonomica Sinica, 2003, 41(4):390-392.
[9]	沈泽昊, 赵子恩. 湖北无心菜属(石竹科)一新种——神农架无心菜[J]. 植物分类学报, 2005, 43(1):73-75. Shen ZH, Zhao ZE. Arenaria shennongjiaensis, a new species of the Caryophyllaceae from Hubei, China[J]. Acta Phytotaxonomica Sinica, 2005, 43(1):73-75.
[10]	姚纲. 石竹科齿瓣无心菜属2新组合名称[J]. 热带亚热带植物学报, 2017, 25(1):11-12. Yao G. Two new combinations in Odontostemma (Caryophyllaceae)[J]. Journal of Tropical and Subtropical Botany, 2017, 25(1):11-12.
[11]	Donoghue MJ, Greenberg AK. Molecular systematics and character evolution in Caryophyllaceae[J]. Taxon, 2011, 60(6):1637-1652.
[12]	Harbaugh DT, Nepokroeff M, Rabeler RK, McNeill J, Zimmer EA, Wagner WL. A new lineage-based tribal classification of the family Caryophyllaceae[J]. Int J Plant Sci, 2010, 171(2):185-198.
[13]	Xu B, Luo D, Li ZM, Sun H. Evolutionary radiations of cushion plants on the Qinghai-Tibet Plateau:insights from molecular phylogenetic analysis of two subgenera of Arenaria and Thylacospermum (Caryophyllaceae)[J]. Taxon, 2019, 68(5):1003-1020.
[14]	罗元霞, 岳学坤, 孙航, 李志敏. 横断山脉地区青藏雪灵芝核形态学[J]. 云南植物研究, 2008, 30(6):662-664. Luo YX, Yue XK, Sun H, Li ZM. Cytological studies on Arenaria roborowskii (Caryophyllaceae) from Hengduan Mountains[J]. Acta Botanica Yunnanica, 2008, 30(6):662-664.
[15]	刘永安, 冯海生, 陈志国, 畅喜云, 刘瑞娟, 等. 物染色体核型分析常用方法概述[J]. 贵州农业科学, 2006, 34(1):98-102. Liu YA, Feng HS, Chen ZG, Chang XY, Liu RJ, et al. Common methods of karyotype analysis in plant[J]. Guizhou Agricultural Sciences, 2006, 34(1):98-102.
[16]	李懋学, 陈瑞阳. 关于植物核型分析的标准化问题[J]. 武汉植物学研究, 1985, 3(4):297-302. Li MX, Chen RY. A suggestion on the standardization of karyotype analysis in plants[J]. Journal of Wuhan Botanical Research, 1985, 3(4):297-302.
[17]	Altinordu F, Peruzzi L, Yu Y, He XJ.A tool for the analysis of chromosomes:KaryoType[J]. Taxon, 2016, 65(3):586-592.
[18]	Tanaka R. Types of resting nuclei in Orchidaceae[J]. Bot Mag-Tokyo, 1971, 84:118-122.
[19]	Levan A, Fredga K, Sandberg AA. Nomenclature for centromeric position on chromosomes[J]. Hereditas, 1964, 52(2):201-220.
[20]	Stebbins GL. Chromosomal Evolution in Higher Plants[M]. London:Edward Arnold Ltd, 1971.
[21]	Arano H. Cytological studies in subfamily Carduoideae (Compositae) of JapanⅪ[STXFZ][J]. Bot Mag, 1963, 76:32.
[22]	Paszko B. A critical review and a new proposal of karyotype asymmetry indices[J]. Plant Syst Evol, 2006, 258:39-48.
[23]	Fadaei F, Sheidai M, Asadi M. Cytological study the genus Arenaria L. (Caryophyllaceae)[J]. Caryologia, 2010, 63(2):149-156.
[24]	Siddique MAA, Jeelani SM. Cyto-genetic diversity with special reference to medicinal plants of the Kashmir Himalaya-a review[J]. Caryologia, 2015, 68(4):365-380.
[25]	王汉屏. 石竹科植物的细胞分类学研究(一)[J]. 陕西教育学院学报, 2004(1):87-91. Wang HP. The cytotaxonomy studies of Caryophyllaceae plants (1)[J]. Journal of Shaanxi Institute of Education, 2004(1):87-91.
[26]	Luo D, Liu D, Xu B, Nie ZL, Sun H, Li ZM. A karyological study of six species of Silene L. (Caryophyllaceae) from the Hengduan Mountains, SW China[J]. Caryologia, 2011, 64(1):10-13.
[27]	底远哲, 胡伸萌, 王海霞, 孙文光, 李志敏. 青藏高原3种蝇子草属植物的核型研究[J]. 植物遗传资源学报, 2019, 20(4):1074-1079. Di YZ, Hu SM, Wang HX, Sun WG, Li ZM. Karyotypes of three Silene L. (Caryophyllaceae) species from the Qinghai-Tibet Plateau[J]. Journal of Plant Genetic Resources, 2019, 20(4):1074-1079.
[28]	杨慧娴, 孙文光, 欧晓昆, 李志敏. 中国西南地区6种植物的核型研究及其系统学意义[J]. 西北植物学报, 2019, 39(4):630-637. Yang HX, Sun WG, Ou XK, Li ZM. Karyotype analysis and systematic significance of six species in Southwest China[J]. Acta Botanica Boreali-Occidentalia Sinica, 2019, 39(4):630-637.
[29]	Löve A, Löve D. Origin and evolution of the arctic and alpine floras[M]//Ives JD, Barry RG, eds. Arctic and Alpine Environment. London:Methuen Co Ltd, 1974.
[30]	王广艳, 孟盈, 聂泽龙, 杨永平. 青藏高原东南缘五种火绒草属植物的核型[J]. 植物分类与资源学报, 2013, 35(3):355-360. Wang GY, Meng Y, Nie ZL, Yang YP. Karyotypes of five Leontopodium species from the Southeastern Qinghai-Tibet Plateau, Southwest China[J]. Plant Diversity and Resources, 2013, 35(3):355-360.
[31]	Meng Y, Yang YP, Sun H, Deng T, Nie ZL. Chromosome numbers, karyotypes, and polyploidy evolution of Anaphalis species (Asteraceae:Gnaphalieae) from the Hengduan Mountains, SW China[J]. Caryologia, 2014, 67(3):238-249.
[32]	Liu JQ. Uniformity of karyotypes in Ligularia (Asteraceae:Senecioneae), a highly diversified genus of the eastern Qinghai-Tibet Plateau highlands and adjacent areas[J]. Bot J Linn Soc, 2004, 144(3):329-342.
[33]	Chen GF, Sun WG, Hong DY, Zhou Z, Niu Y, et al. Systematic significance of cytology in Cyananthus (Campanulaceae) endemic to the Sino-Himalayan region[J]. J Syst Evol, 2014, 52(3):260-270.
[34]	王家坚, 彭智邦, 孙航, 聂泽龙, 孟盈. 青藏高原与横断山被子植物区系演化的细胞地理学特征[J]. 生物多样性, 2017, 25(2):218-225. Wang JJ, Peng ZB, Sun H, Nie ZL, Meng Y. Cytogeographic patterns of angiosperms flora of the Qinghai-Tibet Plateau and Hengduan Mountains[J]. Biodiversity Science, 2017, 25(2):218-225.

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