Identification and Evaluation of Resistance to Bacterial Wilt in Pepper
-
摘要: 采用青枯菌FJC100301菌株对田间辣椒(Capsicum annuum)抗病品种76a 和感病品种TW-1分别作了不同温度、不同接种量和不同接种方法的接种试验。结果表明,辣椒青枯病抗性的室内鉴定以接种温度28℃、浸根20 min和3×108 cfu/mL接种浓度为宜;辣椒种质田间抗青枯病接种鉴定宜选择5月上旬进行,浸根20 min,接种浓度为3×108 cfu/mL。采用田间抗性接种鉴定的方法,用青枯菌FJC100301菌株对106份辣椒材料进行了抗性鉴定。田间接种后每隔10 d统计病情指数,划分辣椒抗青枯病鉴定分级标准,获得了高抗材料14份、抗病材料8份、中抗材料23份、中感材料23份、感病材料20份、高感材料18份;采用离体叶片接种法对田间筛选得到的高抗和高感纯度较高品种进行抗性分析,结果与田间鉴定一致。Abstract: Resistant levels of resistant pepper cultivar (76a) and susceptible pepper cultivar (TW-1) plants were evaluated after infection with Ralstonia solanacearum FJC100301 under different temperatures,concentrations and methods of inoculation.The results showed resistant identification of pepper plants grown in a growth room at 28℃ and infected with 3×108 cfu·mL-1 R.solanacearum suspension through root-soaking at 20 min.Resistant was identified and evaluated in pepper plants grown in the field after inoculation with 3×108 cfu·mL-1 R.solanacearum suspension through root-soaking at 20 min in early May. A total of 106 pepper cultivars were tested in the field after infection with R.solanacearum FJC100301. Disease indexes were scored every 10 day. Meanwhile,standards of resistant levels were divided in pepper. The results showed high resistance in 14 pepper cultivars,resistance in 8 pepper cultivars,moderate resistance in 23 pepper cultivars,moderate susceptibility in 23 pepper cultivars,susceptibility in 20 pepper cultivars,and high susceptibility in 18 pepper cultivars. The same results were obtained in several high resistance and high susceptibility pepper inbred cultivars through leaf inoculation with Ralstonia solanacearum in vitro.
-
Keywords:
- Capsicum annuum /
- Ralstonia solanacearum /
- Germplasm /
- Identification /
- Evaluation
-
-
[1] Wang Y N,Dang F F,Liu Z Q,Wang X,Eulgem T,Lai Y,Yu L,She J J,Shi Y L,Lin J H,Chen C C,Guan D Y,Mou S L,He S L.CaWRKY58,encoding a group Ⅰ WRKY transcription factor of Capsicum annuum,negatively regulates resistance to Ralstonia solanacearum infection [J]. Mol Plant Pathol, 2013, 14(2): 131-144. [2] Dahal D,Heintz D,Van Dorsselaer A,Braun H P,Wydra K. Pathogenesis and stress related,as well as metabolic proteins are regulated in tomato stems infected with Ralstonia solanacearum [J]. Plant Physiol Bioch, 2009, 47(9): 838-846. [3] Hayward A. Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum [J]. Annu Rev Phytopathol, 1991, 29(1): 65-87. [4] 胡青平,徐建国,刘会龙,陈五岭,朱战烨. 番茄茎内生细菌的分离鉴定及青枯病拮抗菌的筛选 [J]. 西北植物学报, 2006(10): 2039-2043. [5] 刘建华,杨宇红,卢鉴植,章志强,王鸣,巩振辉. 辣椒种质资源对疫霉的抗病性鉴定研究 [J]. 湖南农业科学, 1988(3): 30-31. [6] 易图永,张宝玺,谢丙炎,高必达,马新卫. 辣椒疫病三种接种方法的比较 [J]. 中国蔬菜, 2003(2): 16-18. [7] Dang F F,Wang Y N,Yu L,Eulgem T,Lai Y,Liu Z Q,Wang X,Qiu A L,Zhang T X,Lin J,Chen,Y S,Guan D Y,Cai H Y,Mou S L,He S L. CaWRKY40,a WRKY protein of pepper,plays an important role in the regulation of tolerance to heat stress and resistance to Ralstonia solanacearum infection [J]. Plant Cell Environ, 2013, 36, 757-774. [8] 纪成灿,方树民,顾钢,林海,吴正举,陈剑芳. 烟草品种抗青枯病鉴定中的相关因素分析 [J]. 中国烟草科学, 2000(2): 1-4. [9] Afroz A,Khan M R,Ahsan N,Komatsu S. Comparative proteomic analysis of bacterial wilt susceptible and resistant tomato cultivars [J].Peptides, 2009, 30(9): 1600-1607. -
期刊类型引用(5)
1. 伍玉玲, 曾力方, 李火建, 陈珺, 张池, 魏乙斌, 蒋洪涛, 张木清, 姚伟. 中蔗系列甘蔗新品种(系)的耐冷性评价. 广西科学. 2025(02) 
百度学术
2. 蔡博伦，卢永鹏，段维兴，朱俊杰. 以Fv/Fm表征的甘蔗抗寒性与原产地气温的关系. 中国农业气象. 2025(02): 226-236 . 百度学术
3. 李红燕，欧美景，赵秋菊，李佳蔚. 穿刺针直径及校准方法对叶片穿透力机械强度的影响. 广西植物. 2024(03): 564-575 . 百度学术
4. 李秀萍，陆兵，郑平，梁宗余，李楠. 甘蔗果酒补料分批发酵工艺优化及成分分析. 中国食品添加剂. 2023(03): 180-187 . 百度学术
5. 欧美景，李红燕，赵秋菊，李佳蔚. 不同测定方法下叶片穿透力与叶片功能性状之间的关系. 植物研究. 2023(06): 910-922 . 百度学术
其他类型引用(6)
计量
- 文章访问数: 1774
- HTML全文浏览量: 3
- PDF下载量: 1853
- 被引次数: 11
下载:
