木豆凝集素对蚜虫的抗性研究

详细信息

Anti-aphid Effect of Pigeonpea Lectin

1.
Guangxi Crop Improvement and Biotechnology Laboratory, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
2.
Agricultural College, Guangxi University, Nanning 530005, China

摘要: 用盐析法提取10个木豆品种的凝集素,经亲和层析法提纯后添加到全纯人工营养液用于桃蚜饲养,发现所有供试木豆的凝集素对桃蚜都具有不同程度的抗拒性,抗性强弱顺序为:ICP 8863>ICPL 85010>ICPL 99066>ICPL 7035>ICPH 8>ICPL 9008>ICP 6997>“海南种”>ICPL 87091>ICPL 87119。
- 木豆 /
- 凝集素 /
- 抗蚜性
Abstract: Pigeonpea lectin was extracted by ammonium sulfate fractionation.After purified by affinity chromatography on a ConA-Sepharose 4B column,it was added into an artificial holidic diet to feed aphids.The result showed that lectin from all the varieties of pigeonpea had anti-aphid effect to various extensions,which may be sorted descending as ICP 8863>ICPL 85010>ICPL 99066>ICPL 7035>ICPH 8>ICPL 9008>ICP 6997 >HaiNan>ICPL 87091>ICPL 87119.
- Pigeonpea /
- Lectin /
- Anti-aphid