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徐敏, 陈珊, 刘国祥, 胡征宇. 极高CO2胁迫对被甲栅藻(Scenedesmus armatus)生理活性和细胞结构影响[J]. 植物科学学报, 2004, 22(5): 439-444.
引用本文: 徐敏, 陈珊, 刘国祥, 胡征宇. 极高CO2胁迫对被甲栅藻(Scenedesmus armatus)生理活性和细胞结构影响[J]. 植物科学学报, 2004, 22(5): 439-444.
XU Min, CHEN Shan, LIU Guo-Xiang, HU Zheng-Yu. Pilot Study of Physiological and Morphological Acclimation of Scenedesmus armatus Under Extreme-High-CO2-Stress[J]. Plant Science Journal, 2004, 22(5): 439-444.
Citation: XU Min, CHEN Shan, LIU Guo-Xiang, HU Zheng-Yu. Pilot Study of Physiological and Morphological Acclimation of Scenedesmus armatus Under Extreme-High-CO2-Stress[J]. Plant Science Journal, 2004, 22(5): 439-444.

极高CO2胁迫对被甲栅藻(Scenedesmus armatus)生理活性和细胞结构影响

Pilot Study of Physiological and Morphological Acclimation of Scenedesmus armatus Under Extreme-High-CO2-Stress

  • 摘要: 以被甲栅藻(Scenedesmusarmatus)为材料研究极高浓度CO2对其生理活性和细胞结构的影响。研究表明,被甲栅藻能在60%的CO2浓度下快速生长,在5%、20%、40%、60%、80%、100%CO2浓度下的平均增长率分别是1.228、0.925、0.741、0.305、0.042、0.001g·L-1·d-1DW。通入极高浓度CO2(20%、40%)后,被甲栅藻细胞的光系统Ⅱ(PSⅡ)最大光化学效率(Fv/Fm)在24h内明显下降,对PSⅡ抑制作用较明显;其后,随培养时间的增长而逐渐恢复正常。显微结构和亚显微结构显示极高CO2浓度下培养了6d的藻细胞体积稍膨大、颗粒化,色素体结构相对不完整,类囊体膜结构略显松散,蛋白核消失,细胞内的液泡数目增多。

     

    Abstract: Scenedesmus armatus was tested for its high-CO2-tolerant mechanism. It can thrive at 60% CO2 under control. The average growth rates were 1.228, 0.925, 0.741, 0.305, 0.042 and 0.001 g·L-1 ·d-1 DW at 5%,20%,40%,60%,80%,100% CO2 conditions respectively, of which the 20% CO2 is most applicable while 5% is most optimum. The maximum quantum efficiency of photosystemⅡ (PSⅡ) primary photochemistry, given by Fv/Fm=(Fm-F0)/Fm, decreased distinctly in the first 24hrs indicating photoinhibition in PSⅡ, then gradually recovered to the normal levels. The microstructure and ultrastructure of cells growing under air and 20% CO2 indicate some morphological adaptation changes: the chloroplast were not integrated as usual, in which the pyenoids are missing or seldom seen after cultured with high CO2, besides the sizes of the cells and the number of intracellular vacuoles were larger for the cells grown in 20% CO2 for 6 days than for those in air for the same period. These physiological and structural changes may help us to find the way in which algae adapting to the extreme-high-CO2-stress.

     

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