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拟南芥AtDAD1超量表达植株对H2O2抗性的研究

The Role of Arabidopsis AtDAD1 in the Tolerance to Hydrogen Peroxide Stress

  • 摘要: 构建拟南芥AtDAD1超量表达载体,以农杆菌介导的方法转化拟南芥哥伦比亚生态型,比较AtDAD1超量表达植株和野生型植株表现型的差异,以及两者对H2O2抗性的不同。实验显示,AtDAD1转基因拟南芥生长较野生型拟南芥更为强壮,对高浓度H2O2有较强的耐受力。测定两者糖含量,发现AtDAD1转基因拟南芥叶片糖的含量明显高于野生型拟南芥叶片。以上结果表明,AtDAD1基因可能参与植物生长发育,并可能在拟南芥抵抗凋亡的过程中发挥重要的作用。

     

    Abstract: In this research,the differences between wild-type Arabidopsis thaliana and the AtDAD1 overexpression type in their tolerance to high dosages of H2O2 were compared.The diversity on their phenotype from the germination to ripeness was also observed.The results demonstrated that the overexpression of AtDAD1 conferred the transgenic plant more resistant to H2O2 stress.It is also be found that the sugar content in leaves of transgenic plant is much higher than that in wild-type Arabidopsis leaves.Since many lines of evidence have proved that H2O2 is a factor leading to programmed cell death (PCD) and sugar is important for leaf senescence and cell death,it was inferred that AtDAD1 maybe have some effect on Arabidopsis resistance to PCD.

     

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