Identification of Compounds with Allelopathy on Bloom Microalgae from the Secondary Metabolites of Endophytic Fungi Isolated from Marine Macroalgae and the Algicidal Activities

ZUO Jin-Cheng; MIAO Feng-Ping; WANG Ai-Yun; HU De-Chang

doi:10.11913/PSJ.2095-0837.2014.50509

Plant Science Journal > 2014 > 32(5): 509-516. > DOI: 10.11913/PSJ.2095-0837.2014.50509 CSTR: 32231.14.PSJ.2095-0837.2014.50509

ZUO Jin-Cheng, MIAO Feng-Ping, WANG Ai-Yun, HU De-Chang. Identification of Compounds with Allelopathy on Bloom Microalgae from the Secondary Metabolites of Endophytic Fungi Isolated from Marine Macroalgae and the Algicidal Activities[J]. Plant Science Journal, 2014, 32(5): 509-516. DOI: 10.11913/PSJ.2095-0837.2014.50509

Citation:

PDF (698 KB)

Identification of Compounds with Allelopathy on Bloom Microalgae from the Secondary Metabolites of Endophytic Fungi Isolated from Marine Macroalgae and the Algicidal Activities

1. College of Life Sciences, Ludong University, Yantai, Shandong 264025, China;
2. Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, Shandong 264025, China

More Information

Received Date: July 06, 2014
Revised Date: July 20, 2014
Available Online: November 01, 2022
Published Date: October 29, 2014

Graphical Abstract

Abstract

Abstract

To obtain compounds with allelopathy on bloom microalgae, 28 strains of marine macroalgae-endophytic fungi were tested, and four strains in which crude extracts of the secondary metabolites showed apparent allelopathy on Anabaena flos-aquae and Microcystis aeruginosa were obtained. The four strains were cultured in 15 L of PDB culture and the algicidal activities of 32 compounds isolated from the secondary metabolites were examined. The inhibition ratios of sterigmatocystin, (2'E,4'E,6'E)-6-(1'-carboxyocta-2',4',6'-triene)-11,12-epoxy-9,11-dihydroxydrim-7-ene, 6,8-di-O-methylnidurufin and aversin on A. flos-aquae were all significantly higher than 50% (P < 0.05), and the EC_50,24hvalues were 6.865 μg/mL, 4.302 μg/mL, 1.824 μg/mL and 2.319 μg/mL, respectively. The inhibition ratios of sterigmatocystin,(2'E,4'E,6'E)-6-(1'-carboxyocta-2',4',6'-triene)-11,12-epoxy-9,11-dihydroxydrim-7-ene and indol-3-carboxaldehyde on M. aeruginosa were all significantly higher than 50% (P < 0.05), and the EC_50,24h values were 4.167 μg/mL, 8.386 μg/mL and 5.374 μg/mL, respectively.
- Endophytic fungus,
- Antialgal substances,
- Anabaena flos-aquae,
- Microcystis aeruginosa,
- Allelopathy

FullText(HTML)

References (39)

References

[1]	秦伯强,杨柳燕,陈非洲,朱广伟,张路,陈宜瑜.湖泊富营养化发生机制与控制技术及其应用[J].科学通报,2006,57(16):1857-1866.
[2]	鲁志营,高云霓,刘碧云,孙雪梅,张甬元,吴振斌.水生植物化感抑藻作用机制研究进展[J].环境科学与技术,2013,36(7):64-69.
[3]	沈冰洁,陈明利,吴晓芙.利用植物化感作用抑藻的研究进展[J].农业资源与环境学报,2013,30(6):35-39.
[4]	张薛,胡洪营,门玉洁.大麦秆提取液对铜绿微囊藻生长的影响研究[J].环境科学学报,2011,27(12):1984-1987.
[5]	朱擎,冯菁,吴为中,芮克俭,高航.稻草浸泡液的抑藻效果与抑藻活性组分的初步分析[J].北京大学学报,2009,45(1):178-182.
[6]	徐芙清,何伟,郑星,张维昊,蔡伟伟,唐倩.野艾蒿及其有机提取物对铜绿微囊藻生长的抑制作用[J].生态学报,2010,30(3):745-750.
[7]	李锋民,胡洪营,种云霄,门玉洁,郭美婷.芦苇化感物质EMA对铜绿微囊藻生理特性的影响[J].中国环境科学,2007,27(3):377-381.
[8]	高云霓,刘碧云,葛芳杰,梁威,徐栋,张丽萍,吴振斌.三种水鳖科沉水植物释放的脂肪酸类化感物质的分离与鉴定[J].水生生物学报,2011,35(1):170-174.
[9]	胡陈艳,葛芳杰,张胜花,刘碧云,王静,高云霓,吴振斌.马来眼子菜体内抑藻物质分离及常见脂肪酸抑藻效应[J].湖泊科学,2010,22(4):569-576.
[10]	Chen WM, Sheu FS, Sheu SY.Novel L-amino acid oxidase with algicidal activity against toxic cyanobacterium Microcystis aeruginosa synthesized by a bacterium Aquimarina sp.[J].Enzyme Microb Tech, 2011, 49(4):372-379.
[11]	王红强,吴振斌.化感物质的抑藻作用研究、应用及生态安全性评价[J].生态环境学报,2012,21(7):1375-1379.
[12]	戈惠明,谭仁祥.共生菌——新活性天然产物的重要来源[J].化学进展,2009,21(1):30-46.
[13]	Gazis R, Chaverri P.Diversity of fungal endophytes in leaves and stems of wild rubber trees (Hevea brasiliensis) in Peru[J].Fungal Ecol, 2010, 3(3):240-254.
[14]	Zhang Y, Li XM, Proksch P, Wang GB.Ergoste-rimide, a new natural Diels-Alder adduct of a steroid and maleimide in the fungus Aspergillus niger[J].Steroids, 2007, 72(9-10):723-727.
[15]	Cui CM, Li XM, Li CS, Proksch P, Wang BG.Cytoglobosins A-G, cytochalasans from a marine-derived endophytic fungus, Chaetomium globosum QEN-14[J].J Nat Prod, 2010, 73(4):729-733.
[16]	Chen C, Imamura N, Nishijima M, Adachi K, Sakai M, Sano H.Halymecins, new antimicroalgal substances produced by fungi isolated from marine algae[J].J Antibiot, 1996, 49(10):998-1005.
[17]	Krohn K, Dai J, Flörke U, Aust HJ, Dräger S, Schulz B.Botryane metabolites from the fungus Geniculosporium sp.isolated from the marine red alga Polysiphonia[J].J Nat Prod, 2005, 68(3):400-405.
[18]	Ji NY, Liu XH, Miao FP, Qiao MF.Aspeverin, a new alkaloid from an algicolous strain of Aspergillus versiclor[J].Org Lett, 2013, 15(10):2327-2329.
[19]	Rinehart KL, Namikoshi M, Choi BW.Structure and biosynthesis of toxins from blue-green algae(cyanobacteria)[J].J Appl Phycol, 1994, 6(2):159-176.
[20]	Falconer IR, Humpage AR.Tumour promotion by cyanobacterial toxins[J].Phycologia, 1996, 35(6):74-79.
[21]	贾晓会,施定基,史绵红,李仁辉,宋立荣,方昊,虞功亮,李轩,杜桂森.巢湖蓝藻水华形成原因探索及“优势种光合假说”[J].生态学报,2011,31(11):2968-2977.
[22]	El-Jay A.Toxic effects of organic solvents on the growth of Chlorella vulgaris and Selenastrum capricornutum[J].Bull Environ Contam Toxicol, 1996, 57(2):191-198.
[23]	Schrader KK, De Regt MQ, Tucker CS, Duke S.A rapid bioassay for selective algicides[J].Weed Tech, 1997, 11:767-774.
[24]	Liu XH, Tang XZ, Miao FP, Ji NY.A new pyrrolidine derivative and steroids from an algicolous Gibberella zeae strain[J].Nat Prod Commun, 2011, 49(6):1243-1246.
[25]	Liu XH, Miao FP, Li XD, Yin XL, Ji NY.A new sesquiterpene from an endophytic Aspergillus versicolor strain[J].Nat Prod Commun, 2012, 7(7):819-820.
[26]	倪利晓,任高翔,陈世金,郝向阳,李勇,朱亮.酚酸和不饱和脂肪酸对铜绿微囊藻的联合作用[J].环境化学,2011,30(8):1428-1432.
[27]	Liu XH, Miao FP, Qiao MF, Cichewiczb RH, Ji NY.Terretonin, ophiobolin, and drimane terpenes with absolute configurations from an algicolous Aspergillus ustus[J].RSC Adv, 2013, 3(2):588-595.
[28]	Liu XH, Miao FP, Liang XR, Ji NY.Ergosteroid derivatives from an algicolous strain of Aspergillus ustus[J].Nat Prod Res, 2014, 28(15):1182-1186.
[29]	Miao FP, Li XD, Liu XH, Ji NY.Secondary metabolites from an algicolous Aspergillus versicolor strain[J].Mar Drugs, 2012, 10(1):131-139.
[30]	Sun RR, Miao FP, Zhang J, Wang G, Yin XL, Ji NY.Three new xanthone derivatives from an algicolous isolate of Aspergillus wentii[J].Magn Reson Chem, 2013, 51(1):65-68.
[31]	王丰,李厚金,蓝文健.软珊瑚Sarcophyton tor-tuosum共生真菌Penicillium sp.的代谢产物研究[J].中山大学学报:自然科学版,2011,50(6):72-77.
[32]	孙坤来,王乂,付鹏,刘培培,朱伟明.浒苔共生真菌HT-2次生代谢产物的研究[J].中国海洋药物, 2013,32(1):37-45.
[33]	Hehmann A, Kaya K, Watanabe MM.Selective control of microcystis using an amino acid — a laboratory assay[J].J Appl Phycol, 2002, 14(2):85-89.
[34]	Kim JS, Kim JC, Lee S, Lee BH, Cho KY.Biolo-gical activity of L-2-azetidinecarboxylic acid, isolated from Polygonatum odoratum var.pluriflorum, against several algae[J].Aquat Bot, 2006, 85(1):1-6.
[35]	张庭廷,吴安平,何梅,陈传平,聂刘旺.酚酸类物质对水华藻类的化感作用及其机理[J].中国环境科学,2007,27(4):472-476.
[36]	罗万芬,曾仁权.3种长链脂肪酸对水华鱼腥藻的生长影响[J].河南师范大学学报:自然科学版,2011,39(3):119-121.
[37]	花铭,陈良燕,尹大强.邻苯三酚和咖啡酸对铜绿微囊藻的化感作用及其机理[J].环境化学,2008,27(3):331-334.
[38]	王红强.伊乐藻抑藻物质的分离、鉴定及其化感作用研究[D].武汉:中国科学院水生生物研究所,2009.
[39]	赵宝玉,沙湧波.杂色曲霉毒素研究进展[J].动物医学进展,1997,18(2):18-21.

Cited By

Cited by

Periodical cited type(0)

Other cited types(3)

Get Citation

PDF

XML

Article views (1289) PDF downloads (1014) Cited by(3)

Turn off MathJax

Article Contents

Abstract

References

Identification of Compounds with Allelopathy on Bloom Microalgae from the Secondary Metabolites of Endophytic Fungi Isolated from Marine Macroalgae and the Algicidal Activities

Abstract

References

Cited by

Periodical cited type(0)

Other cited types(3)

Catalog

Export File

Citation

Format

Content