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WANG Yan, XIAO Yuan, LIU Wei, LI Ting-Ting, HU Rui, Qiao Zhi-Xian. Operation Skills of Flow Cytometer for Detecting Nuclear DNA Contents in Higher Plant Cells[J]. Plant Science Journal, 2015, 33(1): 126-131. DOI: 10.11913/PSJ.2095-0837.2015.10126
Citation: WANG Yan, XIAO Yuan, LIU Wei, LI Ting-Ting, HU Rui, Qiao Zhi-Xian. Operation Skills of Flow Cytometer for Detecting Nuclear DNA Contents in Higher Plant Cells[J]. Plant Science Journal, 2015, 33(1): 126-131. DOI: 10.11913/PSJ.2095-0837.2015.10126
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Operation Skills of Flow Cytometer for Detecting Nuclear DNA Contents in Higher Plant Cells

  • Analysis and Testing Center, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430070, China

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  • Received Date: March 18, 2014
  • Revised Date: April 23, 2014
  • Available Online: October 31, 2022
  • Published Date: February 27, 2015
    Graphical Abstract
    • Abstract
  • Compared to animals and microorganisms, the application of flow cytometry for plant science is quite difficult in regards to sample preparation, dyeing and testing, which can lead to failure in detection or inaccurate results, due to the special structures and ingredients of plant material (e.g., cell walls, central vacuoles, multifarious organelles and secondary metabolites). Through long-term use of flow cytometry and reference to related literature, we accumulated significant experience in plant science, especially in specimen collection, sample preparation and nuclear DNA content detection. Accordingly, this report provides a valuable reference for plant science and technical personnel engaged in flow cytometry testing.
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    • References (19)
  • [1]
    焦旭雯,赵树进.流式细胞术在高等植物研究中的应用[J].热带亚热带植物学报,2006,14(4): 354-358.
    [2]
    Galbraith DW, Lambert GM, Macas J, Dolezel J. Analysis of nuclear DNA content and ploidy in higher plants[J]. Curr Protoc Cytom, 2001,7(6): 1-22.
    [3]
    Dolezel J, Bartos J. Plant DNA flow cytometry and estimation of nuclear genome size[J]. Ann Bot, 2005, 95(1): 99-110.
    [4]
    Hare EE, Johnston JS. Genome size determination using flow cytometry of propidium iodide-stained nuclei[J]. Methods Mol Biol, 2011,772: 3-12.
    [5]
    Satio H,Mizunashi K,Tanaka S, Adachi Y, Nakano M. Ploidy estimation in Hemerocallis species and cultivars by flow cytometry[J]. Scientia Horticulturae, 2003,97: 185-192.
    [6]
    Suda J, Trávnicek P. Reliable DNA Ploidy determination in dehydrated tissues of vascular plants by DAPI flow cytometry——new prospectsfor plant research[J]. Cytometry A, 2006,69(4): 273-280.
    [7]
    Loureiro J, Rodriguez E, Dolezel J, Santos C. Comparison of four nuclear isolation buffers for plant DNA flow cytometry[J]. Ann Bot, 2006, 98(3): 679-689.
    [8]
    Li ZZ, Man YP, Lan XY, Wang YC. Ploidy and phenotype variation of a natural Actnidia arguta population in the east of Daba Mountain located in a region of Shaanxi[J]. Scientia Horticulturae, 2013,161: 259-265.
    [9]
    Chen JJ, Liu XL, Zhu LY, Wang Y. Nuclear genome size estimation and karyotype analysis of Lycium species[J]. Scientia Horticulturae, 2013,151: 46-50.
    [10]
    Noirot M, Barre P, Duperray C, Louarn J, Hamon S. Effects of caffeine and chlorogenic acid on propidium iodide ac-cessibility to DNA:consequences on genome size evaluation in coffee tree[J]. Ann Bot, 2003, 92(2): 259-264.
    [11]
    Georgiev V, Weber J, Bley T, Pavlov A. Improved procedure for nucleus extraction for DNA measurements by flowcytometry of red beet (Beta vulgaris L.) hairy roots[J]. J Biosci Bioeng, 2009, 107(4): 439-441.
    [12]
    Roux N, Toloza A, Radecke Z, Zapata-Arias FJ, Dolezel J. Rapid detection of aneuploidy in musa using flow cytometry[J]. Plant Cell Rep, 2003,21(5): 483-490.
    [13]
    Phivnil K, Beppu K, Takamura T, Fukuda T, Kataoka I. Flow cytometric assessment of ploidy in native resources of Actinidia in Japan[J]. J Am Pom Soc, 2005, 59(1): 44-49.
    [14]
    Aliyu OM. Development of the flow cytometric protocol for ploidy analysis and determination of relative nuclear DNA content in cashew[J]. Am J Biochem Mol Biol, 2012,2(4): 200-215.
    [15]
    弓娜,田新民,周香艳,刘建全.流式细胞术在植物学研究中的应用——检测植物核DNA含量和倍性水平[J].中国农学通报,2011,27(9): 21-27.
    [16]
    Lee HC, Lin TY. Isolation of plant nuclei suitable for flow cytometry from recalcitrant tissue by use of a filtration column[J]. Plant Mol Bio Rep, 2005,23: 53-58.
    [17]
    于红梅,王静,赵密珍,孟宪凤.利用流式细胞仪检测草莓倍性方法的优化[J].南方农业学报,2012,43(10):1530-1533.
    [18]
    Cousin A, Heel K, Cowling W A, Nelson MN. An efficient high-throughput flow cytometric method for estimating DNA ploidy level in plants[J]. Cytometry, 2009,75(12): 1015-1019.
    [19]
    Dolezel J,Greilhuber J,Lucretti S,Meister A, Lysak MA, Nard L, Obermayer R. Plant genome size estimation by flow cytometry: inter-laboratory comparison[J]. Ann Bot, 1998,82(Supplement A): 17-26.
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