Advance Search
GUO Wen-Fang, LIU De-Chun, YANG Li, ZHUANG Xia, ZHANG Juan-Juan, WANG Shu-Sheng, LIU Yong. Cloning and Expression Analysis of MYB15 Genes from Citrus[J]. Plant Science Journal, 2015, 33(6): 808-818. DOI: 10.11913/PSJ.2095-0837.2015.60808
Citation: GUO Wen-Fang, LIU De-Chun, YANG Li, ZHUANG Xia, ZHANG Juan-Juan, WANG Shu-Sheng, LIU Yong. Cloning and Expression Analysis of MYB15 Genes from Citrus[J]. Plant Science Journal, 2015, 33(6): 808-818. DOI: 10.11913/PSJ.2095-0837.2015.60808
shu

Cloning and Expression Analysis of MYB15 Genes from Citrus

  • Department of Horticulture, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China

More Information
  • Received Date: May 08, 2015
  • Published Date: December 27, 2015
    Graphical Abstract
    • Abstract
  • Three MYB genes, including CmMYB15, PtMYB15 and ClMYB15, were cloned from seedlings of pomelo (Citrus maxima (Burm.) Merr.), trifoliate orange (Poncirus trifoliata (L.) Raf.) and lemon (Citrus limon (L.) Burm.f.), respectively, by in silico and reverse transcription PCR approaches. Results showed that the full length cDNA sequences of the three genes were 994, 992 and 988 bp, and encoded 267, 266 and 265 amino acids, respectively. The N-terminal of the three novel proteins contained two series of incomplete repeat MYB DNA-binding domains, suggesting that the three genes belonged to the R2R3 subclass of the MYB family. Moreover, the expression patterns of these genes were analyzed by real-time quantitative PCR (qRT-PCR) under the stress of ABA, drought, cold and salt.Results indicated that the expressions of the three MYB15 genes were induced by all stress treatments. However, their expression levels after stress differed among the citrus species.The results of this paper suggested that CmMYB15, PtMYB15 and ClMYB15 were members of the MYB gene family, and might play important roles in citrus in response to abiotic stress.
    • FullText(HTML)
    • References (25)
  • [1]
    Singh KB, Foley RC, Oñate-Sanchez L. Transcription factors in plant defense and stress responses[J]. Curr Opin Plant Biol, 2002, 5(5): 430-436.
    [2]
    柳展基, 邵凤霞, 唐桂英. 植物NAC转录因子的结构功能及其表达调控研究进展[J]. 西北植物学报, 2007, 27(9): 1915-1920.
    [3]
    Xu ZS, Chen M, Li LC, Ma YZ. Functions and application of the AP2/ERF transcription factor family in crop improvement[J]. J Integr Plant Biol, 2011, 53(7): 570-585.
    [4]
    Chen YH, Yang XY, He K, Liu MH. The MYB transcription factor superfamily of Arabidopsis: expression analysis and phylogenetic comparison with the rice MYB family[J]. Plant Mol Biol, 2006, 60(1): 107-124.
    [5]
    Martin C, Paz-Ares J. MYB transcription factors in plants[J]. Trends Genet, 1997, 13(2): 67-73.
    [6]
    Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L. MYB transcription factors in Arabidopsis[J]. Trends Plant Sci, 2010, 15(10): 573-581.
    [7]
    Matus JT, Aquea F, Arce-Johnson P. Analysis of the grape MYB R2R3 subfamily reveals expanded wine quality-related clades and conserved gene structure organization across Vitis and Arabidopsis genomes[J]. BMC Plant Biol, 2008, 8(1): 83-98.
    [8]
    Suo JF, Liang XE, Li P, Zhang YS, Xue YB. Identification of GhMYB109 encoding a R2R3 MYB transcription factor that expressed specifically in fiber initials and elongating fibers of cotton (Gossypium hirsutum L.)[J]. BBA-Gene Structure and Expression, 2003, 1630(1): 25-34.
    [9]
    曹忠慧. 苹果MYB转录因子家族的生物信息学分析及MdMYB121和MdSIMYB1在非生物胁迫响应中的作用[D]. 泰安: 山东农业大学, 2013: 26.
    [10]
    胡雪虹, 陈天子, 杨郁文, 张保龙, 王荣富. 棉花MYB转录因子基因GbMYB5的克隆及表达分析[J]. 江苏农业学报, 2012, 28(1): 12-17.
    [11]
    Liao Y, Zou HF, Wang HW, Zhang WK, Ma B, Zhang JS, Chen SY. Soybean GmMYB76, GmMYB92, and GmMYB177 genes confer stress tolerance in transgenic Arabidopsis plants[J]. Cell Res, 2008, 18(10): 1047-1060.
    [12]
    Lee TG, Jang CS, Kim JY, Kim DS, Park JH, Kim DY, Seo YW. A MYB transcription factor (TaMyb1) from wheat roots is expressed during hypoxia: roles in response to the oxygen concentration in root environment and abiotic stresses[J]. Physiol Plantarum, 2007, 129(2): 375-385.
    [13]
    Ding ZH, Li SM, An XL, Liu X, Qin HJ, Wang DW. Transgenic expression of MYB15 confers enhanced sensitivity to abscisic acid and improved drought tolerance in Arabidopsis thaliana[J]. J Genet Genomics, 2009, 36(1): 17-29.
    [14]
    Cultrone A, Cotroneo PS, Recupero GR. Cloning and molecular characterization of R2R3-MYB and bHLH-MYC transcription factors from Citrus sinensis[J]. Tree Genet Genomes, 2010, 6(1): 101-112.
    [15]
    Li ZT, Gmitter FG, Grosser JW, Chen CX, Dennis JG. Isolation and characterization of a novel anthocyanin-promoting MYBA gene family in Citrus[J]. Tree Genet Genomes, 2012, 8(4): 675-685.
    [16]
    Hou XJ, Li SB, Liu SR, Hu CJ, Zhang JZ. Genome-wide classification and evolutionary and expression analyses of citrus MYB transcription factor families in sweet orange[J]. PloS One, 2014, 9(11): e112375.
    [17]
    黄小三. 枳抗逆基因的克隆及功能鉴定[D]. 武汉: 华中农业大学, 2011: 27.
    [18]
    朱丽丽. 柑橘应答低温胁迫的生理生化变化[D]. 武汉: 华中农业大学, 2008.
    [19]
    Riechmann JL, Heard J, Martin G, Reuber L, Jiang C, Keddie J, Adam L, Pineda O, Ratcliffe OJ, Samaha RR, Creelman R, Pilgrim M, Broun P, Zhang JZ, Ghandehari D, Sherman BK, Yu G. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes[J]. Science, 2000, 290(5499): 2105-2110.
    [20]
    刘蕾, 杜海, 唐晓凤, 吴燕民, 黄玉碧, 唐益雄. MYB转录因子在植物抗逆胁迫中的作用及其分子机理[J]. 遗传, 2008, 30(10): 1265-1271.
    [21]
    王雅琴, 石淼, 张新宇, 刘永昌, 薛飞, 孙杰, 李艳军. 棉花GhMYB113基因的克隆与表达分析[J]. 西北植物学报, 2013, 33(5): 878-884.
    [22]
    戴毅, 陆怡, 陈云, 高勇. 玉米MYB家族转录因子基因ZmMYB002的克隆及表达分析[J]. 扬州大学学报: 农业与生命科学版, 2013, 34(1): 60-63.
    [23]
    张立超, 赵光耀, 贾继增, 孔秀英. 小麦盐胁迫相关基因TaMYB32的克隆与分析[J]. 作物学报, 2009, 35(7): 1181-1187.
    [24]
    王春荣, 王庆杰, 李晓玲, 姚玉新, 郝玉金. 葡萄中盐诱导的R2R3-MYB基因的筛选与表达分析[J]. 园艺学报, 2014, 43(3): 529-535.
    [25]
    Xie R, Li Y, He S, Zheng Y, Lv Q. Genome-wide analysis of citrus R2R3 MYB genes and their spatiotemporal expression under stresses and hormone treatments[J]. PloS One, 2014, 9(12): e113971.
    • Related Articles

  • Related Articles

    [1]Fei Yue, Xia Sheng-Ying, Xiong Hai-Yan, Liu Zhi-Xiong. Cloning and expression analysis of the CyfaSTK gene from Cymbidium faberi[J]. Plant Science Journal, 2019, 37(5): 602-609. DOI: 10.11913/PSJ.2095-0837.2019.50602
    [2]Dang Ren-Mei, Zheng Wen-Jie, Ding Ning, Wen Shan-Shan. Cloning and stress expression analysis of the TaHDA19 gene in Triticum aestivum[J]. Plant Science Journal, 2019, 37(4): 495-502. DOI: 10.11913/PSJ.2095-0837.2019.40495
    [3]Zhang Ke-Bin, Chen Bing-Quan, Liu Zhi-Xiong. Cloning and expression analysis of the FaesAP2 gene from Fagopyrum esculentum(Polygonaceae)[J]. Plant Science Journal, 2017, 35(3): 354-361. DOI: 10.11913/PSJ.2095-0837.2017.30354
    [4]Li Li-Chao, Sun Hua-Yu, Lou Yong-Feng, Yang Yi-Hong, Zhao Han-Sheng, Gao Zhi-Min. Cloning and expression analysis of PeLAC in Phyllostachys edulis[J]. Plant Science Journal, 2017, 35(2): 252-259. DOI: 10.11913/PSJ.2095-0837.2017.20252
    [5]FAN Jin-Ping, ZHAO Ran. Cloning and Expression Pattern Analysis of the ApNAP Gene in Asarina procumbens[J]. Plant Science Journal, 2014, 32(3): 251-258. DOI: 10.3724/SP.J.1142.2014.30251
    [6]HUA Wen-Ping, SONG Shuang-Hong, ZHI Yuan, WANG Zhe-Zhi. Cloning and Expression Analysis of the SmGGPPS3 Gene from Salvia miltiorrhiza[J]. Plant Science Journal, 2014, 32(1): 50-57. DOI: 10.3724/SP.J.1142.2014.10050
    [7]YANG Rong-Chao, CAI Yu-Jing, DENG Chun-Ting, OUYANG Bo, YE Zhi-Biao. Cloning of Two Salt-responsive Genes and Their Expression Analysis in Tomato[J]. Plant Science Journal, 2011, 29(2): 178-182.
    [8]LIU Zhen-Lin, CAO Hua-Wen, XIA Xin-Li, YIN Wei-Lun, DAI Si-Lan. Cloning and Expression Analysis of Betaine Aldehyde Dehydrogenase Gene from Dendranthema lavandulifolium on Salinity[J]. Plant Science Journal, 2009, 27(1): 1-7.
    [9]OUYANG Yong-Chang, ZHUANG Dong-Hong, HU Zhong, LI Xiang. Cloning and Prokaryotic Expression of Alpha-momorcharin Gene[J]. Plant Science Journal, 2008, 26(1): 7-11.
    [10]LI Ming-Hui, SUN Ying, ZHAO Chun-Mei, SUN Ai-Qing, HU Xiao-Ran, LIU Jian. Cloning and Stress Expression Analysis of Calnexin in Tomato[J]. Plant Science Journal, 2006, 24(2): 100-105.

Catalog

    Get Citation
    PDF
    XML
    Article views (1534) PDF downloads (1447) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles
    Copyright © 2022 Plant Science Journal 鄂ICP备05004779号-3
    Address：No. 201, Jiufeng 1st Road, Donghu High tech Zone, WuhanPostal Code：430074

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return