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ZHAO Fei-Yi, JIAO Cheng-Jin, JIA Zhen, ZHOU Hui, WANG Mao, ZHAO Lei. Trafficking of AtJ3 from the Nucleus to the Cytoplasm Regulates Plasma Membrane H+-ATPase Activity and ABA Response in Arabidopsis[J]. Plant Science Journal, 2016, 34(3): 406-419. DOI: 10.11913/PSJ.2095-0837.2016.30406
Citation: ZHAO Fei-Yi, JIAO Cheng-Jin, JIA Zhen, ZHOU Hui, WANG Mao, ZHAO Lei. Trafficking of AtJ3 from the Nucleus to the Cytoplasm Regulates Plasma Membrane H+-ATPase Activity and ABA Response in Arabidopsis[J]. Plant Science Journal, 2016, 34(3): 406-419. DOI: 10.11913/PSJ.2095-0837.2016.30406
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Trafficking of AtJ3 from the Nucleus to the Cytoplasm Regulates Plasma Membrane H+-ATPase Activity and ABA Response in Arabidopsis

  • School of Bioengineering & Biotechnology, Tianshui Normal University, Tianshui, Gansu 741000, China

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This work was supported by grants from the National Natural Science Foundation of China (31260568,31160060).

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  • Received Date: December 03, 2015
  • Revised Date: January 04, 2016
  • Available Online: October 31, 2022
  • Published Date: June 27, 2016
    Graphical Abstract
    • Abstract
  • Arabidopsis thaliana DnaJ homolog 3 (AtJ3), an Arabidopsis chaperone, interacts with SOS2-like protein kinase 5 (PKS5) to form the AtJ3-PKS5 complex and achieves its function through repressing activity of PKS5 in vivo. Moreover, the AtJ3-PKS5 complex positively regulates plasma membrane H+-ATPase activity and is involved in exogenous ABA response in Arabidopsis. In this study, salt- and ABA-treated Arabidopsis AtJ3 and PKS5 mutants were explored to elucidate the function and mechanism of the AtJ3-PKS5 complex in the simultaneous regulation of membrane H+-ATPase activity and ABA response. Results showed that the AtJ3-PKS5 complex not only led to changes in the cytosolic pH value via regulation of plasma membrane H+-ATPase activity, but also activated expression of the ABA-related responsive genes under the two treatments. Additionally, exogenous application of ABA induced trafficking of AtJ3 from the nucleus to the cytoplasm to enhance H+-ATPase activity, indicating that the AtJ3-PKS5 complex functions in ABA-meditated pH homeostasis. Taken together, our results suggest that the AtJ3-PKS5 complex might serve as a key regulator in a crosstalk metabolic pathway of H+-ATPase activity regulation and exogenous ABA response.
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    • References (28)
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