Advance Search
GUO Xiao-Rong, YANG Xin-Bing, WANG Huai-Qin, MING Fang-Lin, SHE Xu, CAO Xiao-Yan. Cloning and Expression Analysis of miR408 Precursor Sequences from Salvia miltiorrhiza[J]. Plant Science Journal, 2016, 34(3): 430-438. DOI: 10.11913/PSJ.2095-0837.2016.30430
Citation: GUO Xiao-Rong, YANG Xin-Bing, WANG Huai-Qin, MING Fang-Lin, SHE Xu, CAO Xiao-Yan. Cloning and Expression Analysis of miR408 Precursor Sequences from Salvia miltiorrhiza[J]. Plant Science Journal, 2016, 34(3): 430-438. DOI: 10.11913/PSJ.2095-0837.2016.30430
shu

Cloning and Expression Analysis of miR408 Precursor Sequences from Salvia miltiorrhiza

  • Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an 710062, China

Funds: 

This work was funded by grants from the Natural Science Foundation of Shaanxi Province (2014JQ3107), Fundament Research Funds for the Central Universities (GK201302043), and Shaanxi Normal University Work-Study Program For Scientific Research Innovation Fund (KY2015ZD05).

undefined

undefined

More Information
  • Received Date: January 06, 2016
  • Revised Date: January 31, 2016
  • Available Online: October 31, 2022
  • Published Date: June 27, 2016
    Graphical Abstract
    • Abstract
  • MiR408 is a highly conserved miRNA in plants and responds to copper availability by targeting genes encoding copper-containing proteins. Its expression is significantly affected by plant growth and development and environmental conditions. MiR408 is critical for the HY5-SPL7 gene network in Arabidopsis, and mediates a coordinated response to light and copper, illustrating its central role in the reaction of plants to the environment. Based on the genomic survey database of Salvia miltiorrhiza established in our lab, a 366 bp miR408 precursor sequence with stable stem loop structure was cloned and named Sm-MIR408. The 723 bp promoter region upstream of Sm-MIR408 was obtained by PCR. The GenBank accession numbers of Sm-MIR408 and the promoter sequence are KU360384 and KU360385, respectively. Bioinformatic analysis showed that the promoter region of Sm-MIR408 shared the same cis-acting elements as those of Ath-MIR408 in Arabidopsis thaliana and Osa-MIR408 in Oryza sativa, and included G-box, CGTCA-motif, TGACG-motif and GTAC-motif, whereas HSE and CATT-motif elements existed only in the Sm-MIR408 promoter region. Mature miR408 is highly conserved among different species. A phylogenetic tree of miR408 from different species showed that miR408 precursor sequences from monocotyledons were clustered on one branch and those from dicotyledons were clustered on another. Real-time quantitative PCR showed that the expression of Sm-MIR408 in the roots, stems, leaves and flowers of S. miltiorrhiza was the highest in the flowers and the lowest in the roots. Expression of Sm-MIR408 could be suppressed by methyl jasmonate, wounding, and continuous light or darkness. The cloning and expression analysis of Sm-MIR408 presented in this research has laid a foundation for studying the function of miR408 in S. miltiorrhiza in the future.
    • FullText(HTML)
    • References (28)
  • [1]
    Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function[J]. Cell, 2004, 116: 281-297.
    [2]
    Axtell MJ, Bowman JL. Evolution of plant microRNAs and their targets[J]. Trends Plant Sci, 2008, 13: 343-349.
    [2]
    Axtell MJ, Bowman JL. Evolution of plant microRNAs and their targets[J]. Trends Plant Sci, 2008, 13: 343-349.
    [3]
    方良,梁远学,李东栋,曹献英,郑育声.油棕( Elaeis guineensis)中果皮发育过程中miRNA的表达动态分析[J].植[1] Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function[J]. Cell, 2004, 116: 281-297.
    [3]
    方良,梁远学,李东栋,曹献英,郑育声.油棕( Elaeis guineensis)中果皮发育过程中miRNA的表达动态分析[J].植物科学学报, 2013, 31(3) : 304-312.

    Fang L, Liang YX, Li DD, Cao XY, Zheng YS. Dynamic expression analysis of miRNAs during the development process of oil palm mesocarp[J]. Plant Science Journal, 2013, 31(3): 304-312.
    [4]
    Jones-Rhoades MW, Bartel DP, Bartel B. MicroRNAS and their regulatory roles in plants[J]. Annu Rev Plant Biol, 2006, 57: 19-53.
    [5]
    Ramachandran V, Chen XM. Small RNA metabolism in Arabidopsis[J]. Trends Plant Sci, 2008, 13: 368-374.
    [6]
    Carrington JC, Ambros V. Role of microRNAs in plant and animal development[J]. Science, 2003, 301: 336-338.
    [7]
    Chen XM. Small RNAs and their roles in plant development[J]. Annu Rev Cell Dev Biol, 2009,25(25):21-44.
    [8]
    Sunkar R. MicroRNAs with macro-effects on plant stress responses[J]. Semin Cell Dev Biol, 2010, 21: 805-811.
    [9]
    Jones-hoades MW, Bartel DP, Bartel B. MicroRNAs and their regulatory roles in plants[J]. Annu Rev Plant Biol, 2006, 57: 19-53.
    [10]
    Zhang H, Li L. SQUAMOSA promoter binding protein-like7 regulated microRNA408 is required for vegetative development in Arabidopsis[J]. Plant J, 2013, 74(1): 98-109.
    [11]
    Kantar M, Unver T, Budak H. Regulation of barley miRNAs upon dehydrationstress correlated with target gene expression[J]. Funct Integr Genom, 2010, 10(4): 493-507.
    [12]
    Lu S, Sun YH, Shi R, Clark C, Li L, Chiang VL. Novel and mechanical stressresponsive microRNAs in Populus trichocarpa that are absent from Arabidopsis[J]. Plant Cell, 2005, 17(8): 2186-2203.
    [13]
    Li T, Li H, Zhang YX, Liu JY. Identification and analysis of seven H2O2-responsive miRNAs and 32 new miRNAs in the seedlings of rice(Oryza sativa L. ssp. indica)[J]. Nucleic Acids Res, 2011, 39(7): 2821-2833.
    [14]
    Trindade I, Capitão C, Dalmay T, Fevereiro MP, Santos DM. miR398 and miR408 are up-regulated in response to water deficit in Medicago truncatula[J]. Planta, 2010, 231(3): 705-716.
    [15]
    Kantar M, Unver T, Budak H. Regulation of barley miRNAs upon dehydrationstress correlated with target gene expression[J]. Funct Integr Genom, 2010, 10(4): 493-507.
    [16]
    Eldem V, Akçay UÇ, Ozhuner E, Bakır Y, Uranbey S, Unver T. Genome-wide identification of miRNAs responsive to drought in peach(Prunus persica) by high throughput deep sequencing[J]. PloS One, 2012, 7: e50298.
    [17]
    Zhou L, Liu Y, Liu Z, Kong D, Duan M, Luo L. Genome-wide identification andanalysis of drought-responsive microRNAs in Oryza sativa[J]. J Exp Bot, 2010, 61(15): 4157-4168.
    [18]
    Zhang H, He H, Wang X, Wang X, Yang X, Li L, Deng XW. Genome-wide mapping of the HY5-mediated gene networks in Arabidopsis that involve both transcriptional and post-transcriptional regulation[J]. Plant J, 2011, 65:346-358.
    [19]
    Ma C, Burd S, Lers A. miR408 is involved in abiotic stress responses in Arabidopsis[J]. Plant J, 2015, 84:169-187.
    [20]
    化文平,宋双红,智媛,王喆之.丹参SmGGPPS3基因的克隆及表达分析[J].植物科学学报,2014, 32(1): 50-57.

    Hua WP, Song SH, Zhi Y, Wang ZZ. Cloning and expression analysis of the SmGGPPS3 gene from Salvia miltiorrhiza[J]. Plant Science Journal, 2014, 32(1): 50-57.
    [21]
    Xu X, Jiang Q, Ma X, Ying Q, Shen B, Qian Y, Song H, Wang H. Deep sequencing identifies tissue-specific microRNAs and their target genes involving in the biosynthesis of tanshinones in Salvia miltiorrhiza[J]. PloS One, 2014, 9: e111679.
    [22]
    张媛. MeJA和PAP1转录因子对丹参酚酸类成分积累的影响[D].陕西:陕西师范大学, 2011: 66. Zhang Y. Transcription factor PAP1 and MeJA have an effect on accumulation of phenolic acids of Salvia miltiorrhiza[D]. Shaanxi: Shaanxi Normal University, 2011:66.
    [23]
    Schuetz M, Benske A, Smith RA, Watanabe Y, Tobimatsu Y, Ralph J, Demura T, Ellis B, Samuels AL. Laccases direct lignification in the discrete secondary cell wall domains of protoxylem[J]. Plant Physiol, 2014, 166: 798-807.
    [24]
    Zhang HY, Zhao X, Li JG., Cai HQ, Deng XW,Li L. MicroRNA408 is critical for the HY5-SPL7 gene network that mediatesthe coordinated response to light and copper[J]. Plant Cell, 2014, 26: 4933-4953.
    [25]
    Gou JY, Felippes FF, Liu CJ, Weigel D, Wang JW. Negative regulation of anthocyanin biosynthesis in Arabidopsis by a miR156-targeted SPL transcription factor[J]. Plant Cell, 2011, 23:1512-1522.
    [26]
    Ng DW, Zhang C, Miller M, Palmer G, Whiteley M, Tholl D, Chen ZJ. cis-and trans-regulation of miR163 and target genes confers natural variation of secondary metabolites in two Arabidopsis species and their allopolyploids[J]. Plant Cell, 2011, 23:1729-1740.物科学学报, 2013, 31(3) : 304-312.

    Fang L, Liang YX, Li DD, Cao XY, Zheng YS. Dynamic expression analysis of miRNAs during the development process of oil palm mesocarp[J]. Plant Science Journal, 2013, 31(3): 304-312.
    • Related Articles

  • Related Articles

    [1]Yuan Pu-Ying, Song Xing-Rong, Shao Yi-Fan. Study on cell structure, physiology, and biochemistry of vascular bundle blackening in petals of Chimonanthus praecox (L.) Link[J]. Plant Science Journal, 2019, 37(6): 781-787. DOI: 10.11913/PSJ.2095-0837.2019.60781
    [2]HU Jian-Zhu, ZOU Pu, WANG Xiao-Bin, LIAO Jing-Ping. Floral Vascular System Anatomy of Heliconia rostrata(Heliconiaceae)[J]. Plant Science Journal, 2011, 1(5): 537-543.
    [3]HE Yu-Chi, TANG Xing-Chun, HE Yu-Qing, SUN Meng-Xiang. Roles of Cell Wall in Cell Polarity Establishment and Embryogenesis[J]. Plant Science Journal, 2006, 24(5): 464-468.
    [4]CHEN Shun-Qiang, WANG Yang, ZHANG Zhi-Hong, WANG Ling-Xia, HU Zhong-Li, LI Ping, ZHU Li-Huang, ZHU Ying-Guo. Genetic Dissection of Vascular Bundle Systems in Peduncle and Panicle Characters in Rice (Oryza sativa L.) by Means of RFLP Markers[J]. Plant Science Journal, 2004, 22(1): 15-21.
    [5]TANG Yuan-Jiang, LIAO Jing-Ping. Studies on Vascular System Anatomy of the Flower of Commelina communis L.[J]. Plant Science Journal, 2001, 19(2): 96-100.
    [6]Li Wei. FLORA STUDIES ON AQUATIC VASCULAR PLANTS IN LAKE HONGHU[J]. Plant Science Journal, 1997, 15(2): 113-122.
    [7]Bi Liejue. A QUESTION ABOUT A BASAL CELL[J]. Plant Science Journal, 1994, 12(2): 185-188.
    [8]Hu ChuanJiong, Zhou Pingzhen, Chen Huakui. THE STUDY ON MORPHOLOGICAL DEVELOPMENT OF NODULAR VASCULAR BUNDLES OF CORIARIA NEPALENSIS WALL.[J]. Plant Science Journal, 1993, 11(3): 211-214.
    [9]Xu Zhenxiu, Hu Chunkui, Lan Shengyin. A TECHNIQUE OF OBSERVATION ON PARAFFIN SECTIONS WITH SCANNING ELECTRON MICROSCOPE[J]. Plant Science Journal, 1992, 10(4): 377-380.
    [10]Feng Can, Wang Xuelei, Wang zengxue, Ban Jide. STUDIES ON THE COMMUNITIES OF AQUATIC VASCULAR PLANTS IN CHANGHU LAKE[J]. Plant Science Journal, 1989, 7(2): 123-130.

  • Cited by

    Periodical cited type(7)

    1. 蔡焕满，吴素美，吴逸卿，熊艳云，林阳，毛志斌，吴友贵，吴秋丰. 百山祖国家公园五岭坑常绿阔叶林甜槠的种群特征. 浙江林业科技. 2024(01): 1-7 .
    2. 黎毅，戴克元，唐国平，杜建会，陈桃，江南，牛香豫，余扬波. 基于遥感生态指数的广东石门台国家级自然保护区生态环境质量评价. 热带地理. 2024(03): 429-441 .
    3. 邹艳丽，王倩，丁巧玲，周奇，刘忠成，陈志晖，廖文波. 罗霄山脉甜槠(Castanopsis eyrei)群落的纬度地带性研究. 生态科学. 2023(06): 93-104 .
    4. 张银，吴浩，徐耀粘，王世彤，杨腾，李晶，吕林玉，周天阳，肖之强，王建，江明喜. 湖北九宫山甜槠群落结构与优势种的空间分布格局研究. 长江流域资源与环境. 2021(05): 1130-1140 .
    5. 娄明华，杨同辉，陈文伟，许俊. 宁波天然甜槠阔叶混交林树高—胸径模型研究. 防护林科技. 2021(05): 1-5 .
    6. 周茹君，曾颖，梁小翠，闫文德，张翔. 湖南芦头林场甜槠天然林群落结构特征. 湖南林业科技. 2020(04): 85-91 .
    7. 杨礼旦，陈应强，杨学成. 贵州台江县野生木本资源及其利用分析. 山地农业生物学报. 2020(05): 67-77 .

    Other cited types(1)

Catalog

    Get Citation
    PDF
    XML
    Article views (1409) PDF downloads (1402) Cited by(8)
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles
    Copyright © 2022 Plant Science Journal 鄂ICP备05004779号-3
    Address：No. 201, Jiufeng 1st Road, Donghu High tech Zone, WuhanPostal Code：430074

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return