Effects of Spraying Cytokinin on Abscission Rate and Enzymic Activity of Flowers and Pods of Cowpea

HU Zhi-Hui; WANG Yan-Jie; CHEN Chan-You

doi:10.11913/PSJ.2095-0837.2016.30439

Plant Science Journal > 2016 > 34(3): 439-445. > DOI: 10.11913/PSJ.2095-0837.2016.30439 CSTR: 32231.14.PSJ.2095-0837.2016.30439

HU Zhi-Hui, WANG Yan-Jie, CHEN Chan-You. Effects of Spraying Cytokinin on Abscission Rate and Enzymic Activity of Flowers and Pods of Cowpea[J]. Plant Science Journal, 2016, 34(3): 439-445. DOI: 10.11913/PSJ.2095-0837.2016.30439

Citation:

PDF (788 KB)

Effects of Spraying Cytokinin on Abscission Rate and Enzymic Activity of Flowers and Pods of Cowpea

College of Life Sciences, Jianghan University, Hubei Province Engineering Research Center for Legume Plants, Wuhan 430056, China

Funds:

This work was supported by grants from the Ministry of Agriculture ‘948’ Project (2011-G1-17), Science and Technology Platform Project in Hubei Province (Hubei tech-savvy [2011] No. 101), Wuhan Science and Technology Plan Projects (201250499145-12), and Wuhan Yellow Crane Talents (Agricultural) Program (2014).

undefined

More Information

Received Date: December 23, 2015
Revised Date: January 28, 2016
Available Online: October 31, 2022
Published Date: June 27, 2016

Graphical Abstract

Abstract

Abstract

Four cowpea varieties, ‘E Jiangdou 2’, ‘E Jiangdou 6’, ‘E Jiangdou 7’, and ‘Meiguo Didou’, provided by the Hubei Province Engineering Research Center were selected as research materials. We sprayed cytokinin on leaves in budding stage to study its effects on the abscission of flowers and pods in the field. At 7, 14, 28 and 42 d after spraying, we analyzed the yields and abscission rates of flowers and pods, and detected the enzyme activities of polygalacturonase and cellulase in premature shedding of flowers and pods.Results showed that after spraying cytokinin, the abscission rates of flowers and pods in different cultivars were lower than those in the control, but yields in different cultivars were higher, with the differences being highly significant (P < 0.01). Furthermore, the polyga-lacturonase activities of cowpea were significantly lower than those of the control at 14 d, 28 d and 42 d after spraying (P < 0.05), and significantly lower at 7 d after spraying (P < 0.01). The cellulase activities of fallen flowers and pods were significantly (P < 0.05) lower than those of the control at 7 d and 42 d after spraying, and significantly (P < 0.01) lower at 14 d and 28 d after spraying. The above results indicated that the activities of polygalacturonase and cellulase were regulated by spraying cytokinin, which resulted in reducing the abscission rate of flowers and pods and regulating yield in cowpea.
- Cowpea,
- Cytokinins,
- Flowers and pods,
- Abscission rate,
- Polygalacturonase,
- Cellulase

FullText(HTML)

References (19)

References

[1]	党江波,李彩,李晔,龙志坚,魏旭,汪卫星,何桥,郭启高,梁国鲁. 豇豆螺旋茎段维管组织观察及其成因分析[J]. 植物科学学报,2011, 29(6) : 743-748. Dang JB, Li C, Li Y, Long ZJ, Wei X, Wang WX, He Q, Guo QG, Liang GL. Observation and genesis analysis of vascular tissue in cowpea's helix stem[J]. Plant Science Journal,2011, 29(6) : 743-748.
[2]	Taylor JE, Whitelaw CA. Signals in abscission[J]. New Phytologist, 2001, 151(2): 323-339.
[3]	Clements JC, Atkins CA. Characterization of a nonab-scission mutant in Lupinus angustifolius L.: Physiological aspects[J]. Annals of Botany, 2001, 88(4) : 629- 635.
[4]	Bonghi C, Rascio N, Ramina A, Casadoro G. Cellulase and polygalacturonase involvement in the abscission of leaf and fruit explants of peach[J]. Plant Mol Biol, 1992, 20(5): 839- 848.
[5]	崔洪秋,冯乃杰,孙福东, 刘涛, 李建英,杜吉到,韩毅强,郑殿峰. DTA-6 对大豆花荚脱落纤维素酶和 GmAC基因表达的调控[J].作物学报, 2016, 42(1): 51- 57. Cui HQ, Feng NJ, Sun FD, Liu T, Li JY, Du JD, Han YQ, Zheng DF. Regulation of DTA-6 by abscission cellulase and GmAC gene expression in flowers and pods of soybean[J]. Acta Agronomica Sinica, 2016, 42(1): 51-57.
[6]	Kemmerer EC,Tucker ML.Comparative study of cellulase associated with adventitious root initiation,apical buds and leaf flower and pod abscission zones in soybean[J].Plant Physiol, 1994,104(2): 557-562.
[7]	李辉亮.激素和热激对大豆(Glycine max (L.) Merrill)花荚脱落的影响及其分子机理研究[D].长沙: 湖南农业大学, 2004. Li HL. Studies on the effects of hormone and heat shock on soybean (Glycine max (L.) Merrill) legume abscission and its molecule mechanism[D]. Changsa: Hunan Agricultural University, 2004.
[8]	刘大林,谷文英,秦玉玲,吕勇,吴敏,王留香. 外源细胞分裂素对紫花苜蓿生长及品质的影响[J]. 草业科学,2005, 22(10):36-40. Liu DL, Gu WY, Qin YL, Lü Y, Wu M, Wang LX. Effects of exogenous CTK on the growth and character of alfalfa (Medicago sativa) plants[J]. Pratacultural Science, 2005, 22 (10):36-40.
[9]	高金山,边庆华,张永忠,王敏. 细胞分裂素CPPU的研究进展[J],农药,2006,45(3): 151-154. Gao JS, Bian QH, Zhang YZ, Wang M. Research progress on the cytokinin, CPPU[J]. Agrochemicals, 2006, 45(3): 151-154.
[10]	宋莉萍,刘金辉,郑殿峰,冯乃杰. 不同时期叶喷植物生长调节剂对大豆花荚脱落率及多聚半乳糖醛酸酶活性的影响[J]. 植物生理学报,2011, 47(4): 356-362. Song LP, Liu JH, Zheng DF, Feng NJ. Effects of different plant growth regulators on abscission rate and polygalacturonase activities of soybean flowers and pods by spraying in different stages[J]. Plant Physiology Journal, 2011, 47(4): 356-362.
[11]	汪宝卿,慈敦伟,张礼凤,李伟,徐冉.同化物供应和内源激素信号对大豆花荚发育的调控[J]. 大豆科学, 2010, 29 (5):878-882. Wang BQ, Ci DW, Zhang LF, Li W, Xu R. Research progress of assimilation supply and endogenous hormones signals regulation involved in flower and pod deve-lopment of soybean[J]. Soybean Science, 2010, 29 (5):878-882.
[12]	赵双进,唐晓东,赵鑫,冯燕,赵聪聪,张孟臣. 大豆开花落花及时空分布的观察研究[J].中国农业科学, 2013, 46(8): 1543-1554. Zhao SJ, Tang XD, Zhao X, Feng Y, Zhao CC, Zhang MC. Observation and research on the temporal and spatial distribution of flowering and flower dropping of soybean[J]. Scientia Agricultura Sinica, 2013, 46(8): 1543-1554.
[13]	孙福东.两种调节剂对无限结荚习性大豆花荚建成的调控及机制[D].大庆市: 黑龙江八一农垦大学,2015. Sun FD. Mechanism and regulation of two kind of plant growth regulators on flowers and pods formation of indeterminate growth habit soybean[D]. Daqing: Heilongjiang Bayi Agricultural University, 2015.
[14]	Oko BFD, Eneji AE, Binang W,Irshad M, Yamamoto S, Honna T, Endo T. Effect of foliar application of urea on reproductive abscission and grain yield of soybean[J]. J Plant Nutr, 2003, 26 (6) :1223-1234.
[15]	柴国华. 激素、逆境对大豆脱落纤维素酶基因表达的调控及其对花荚脱落的影响[D]. 杨凌:西北农林科技大学, 2006. Cai GH. Regulation of abscission cellulose gene expression and effect of flowers and pods abscission in soybean by hormones or unfavorable circumstances[D].Yangling: Northwest Agricultural and Forestry University, 2006.
[16]	张海峰, 张明才,翟志席, 段留生,李召虎,黄淮,陈刚. SHK-6对不同群体下大豆花荚脱落及其产量的调控[J]. 大豆科学,2007, 26(1):78-83. Zhang HF, Zhang MC, Zhai ZX, Duan LS, Li ZH, Huang Z, Chen G. Effect of SHK-6 application on abscission of flower and pod and yield in different density[J]. Soybean Science, 2007, 26(1):78-83.
[17]	宋莉萍,刘金辉,郑殿峰,冯乃杰. 不同时期PGRs对大豆花荚脱落率及纤维素酶活性的影响[J]. 中国油料作物学报,2011, 33(3):253-258. Song LP, Liu JH, Zheng DF, Feng NJ. Regulation of plant growth regulators on abscission rate and cellulose activity of soybean flowers and pods[J]. Chinese Journal of Oil Crop Sciences, 2011, 33(3):253-258.
[18]	尹宝重, 刘盼, 张月辰. 红小豆花器官脱落对短日照诱导的生理响应[J]. 作物杂志, 2015(1): 78-85. Yin BZ, Liu P, Zhang YC. The physiological response of floral organ abscission for adzuki bean (Vigna angularis) under short daylenth photoperiod induction[J]. Crops, 2015(1): 78-85.
[19]	许涛.钙素对番茄花柄外植体脱落调控机理的研究[D].沈阳: 沈阳农业大学,2008. Xu T. Study on regulating mechanism of calcium on the abscission of tomato pedicel[D]. Shenyang: Shenyang Agricultural University, 2008.

[1]	Yuan Pu-Ying, Song Xing-Rong, Shao Yi-Fan. Study on cell structure, physiology, and biochemistry of vascular bundle blackening in petals of Chimonanthus praecox (L.) Link[J]. Plant Science Journal, 2019, 37(6): 781-787. DOI: 10.11913/PSJ.2095-0837.2019.60781
[2]	HU Jian-Zhu, ZOU Pu, WANG Xiao-Bin, LIAO Jing-Ping. Floral Vascular System Anatomy of Heliconia rostrata(Heliconiaceae)[J]. Plant Science Journal, 2011, 1(5): 537-543.
[3]	HE Yu-Chi, TANG Xing-Chun, HE Yu-Qing, SUN Meng-Xiang. Roles of Cell Wall in Cell Polarity Establishment and Embryogenesis[J]. Plant Science Journal, 2006, 24(5): 464-468.
[4]	CHEN Shun-Qiang, WANG Yang, ZHANG Zhi-Hong, WANG Ling-Xia, HU Zhong-Li, LI Ping, ZHU Li-Huang, ZHU Ying-Guo. Genetic Dissection of Vascular Bundle Systems in Peduncle and Panicle Characters in Rice (Oryza sativa L.) by Means of RFLP Markers[J]. Plant Science Journal, 2004, 22(1): 15-21.
[5]	TANG Yuan-Jiang, LIAO Jing-Ping. Studies on Vascular System Anatomy of the Flower of Commelina communis L.[J]. Plant Science Journal, 2001, 19(2): 96-100.
[6]	Li Wei. FLORA STUDIES ON AQUATIC VASCULAR PLANTS IN LAKE HONGHU[J]. Plant Science Journal, 1997, 15(2): 113-122.
[7]	Bi Liejue. A QUESTION ABOUT A BASAL CELL[J]. Plant Science Journal, 1994, 12(2): 185-188.
[8]	Hu ChuanJiong, Zhou Pingzhen, Chen Huakui. THE STUDY ON MORPHOLOGICAL DEVELOPMENT OF NODULAR VASCULAR BUNDLES OF CORIARIA NEPALENSIS WALL.[J]. Plant Science Journal, 1993, 11(3): 211-214.
[9]	Xu Zhenxiu, Hu Chunkui, Lan Shengyin. A TECHNIQUE OF OBSERVATION ON PARAFFIN SECTIONS WITH SCANNING ELECTRON MICROSCOPE[J]. Plant Science Journal, 1992, 10(4): 377-380.
[10]	Feng Can, Wang Xuelei, Wang zengxue, Ban Jide. STUDIES ON THE COMMUNITIES OF AQUATIC VASCULAR PLANTS IN CHANGHU LAKE[J]. Plant Science Journal, 1989, 7(2): 123-130.

Cited By

Cited by

Periodical cited type(7)

1.	蔡焕满，吴素美，吴逸卿，熊艳云，林阳，毛志斌，吴友贵，吴秋丰. 百山祖国家公园五岭坑常绿阔叶林甜槠的种群特征. 浙江林业科技. 2024(01): 1-7 .
2.	黎毅，戴克元，唐国平，杜建会，陈桃，江南，牛香豫，余扬波. 基于遥感生态指数的广东石门台国家级自然保护区生态环境质量评价. 热带地理. 2024(03): 429-441 .
3.	邹艳丽，王倩，丁巧玲，周奇，刘忠成，陈志晖，廖文波. 罗霄山脉甜槠(Castanopsis eyrei)群落的纬度地带性研究. 生态科学. 2023(06): 93-104 .
4.	张银，吴浩，徐耀粘，王世彤，杨腾，李晶，吕林玉，周天阳，肖之强，王建，江明喜. 湖北九宫山甜槠群落结构与优势种的空间分布格局研究. 长江流域资源与环境. 2021(05): 1130-1140 .
5.	娄明华，杨同辉，陈文伟，许俊. 宁波天然甜槠阔叶混交林树高—胸径模型研究. 防护林科技. 2021(05): 1-5 .
6.	周茹君，曾颖，梁小翠，闫文德，张翔. 湖南芦头林场甜槠天然林群落结构特征. 湖南林业科技. 2020(04): 85-91 .
7.	杨礼旦，陈应强，杨学成. 贵州台江县野生木本资源及其利用分析. 山地农业生物学报. 2020(05): 67-77 .

Other cited types(1)

Get Citation

PDF

XML

Article views (1123) PDF downloads (1188) Cited by(8)

Turn off MathJax

Article Contents

Abstract

References

Effects of Spraying Cytokinin on Abscission Rate and Enzymic Activity of Flowers and Pods of Cowpea

Abstract

References

Related Articles

Cited by

Periodical cited type(7)

Other cited types(1)

Catalog

Effects of Spraying Cytokinin on Abscission Rate and Enzymic Activity of Flowers and Pods of Cowpea

Abstract

References

Related Articles

Cited by

Periodical cited type(7)

Other cited types(1)

Catalog

Export File

Citation

Format

Content