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LI Yan, GONGYE Xiang-Xu, ZHU Hui-Jun, ZHAO Lei, YANG Yu-Qi, ZHANG Xing. Preliminary Study on Two-phase Culture of Tripterygium wilfordii Adventitious Root[J]. Plant Science Journal, 2016, 34(3): 469-474. DOI: 10.11913/PSJ.2095-0837.2016.30469
Citation: LI Yan, GONGYE Xiang-Xu, ZHU Hui-Jun, ZHAO Lei, YANG Yu-Qi, ZHANG Xing. Preliminary Study on Two-phase Culture of Tripterygium wilfordii Adventitious Root[J]. Plant Science Journal, 2016, 34(3): 469-474. DOI: 10.11913/PSJ.2095-0837.2016.30469
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Preliminary Study on Two-phase Culture of Tripterygium wilfordii Adventitious Root

  • 1. College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China;

  • 2. Research and Development Center of Biorational Pesticide, Northwest A&F University/Shaanxi Province Technology and Engineering Center of Biopesticides, Yangling, Shaanxi 712100, China

Funds: 

This work was supported by grants from the National Natural Science Foundation of China (31272110) and National High Techno-logy Research and Development Program of China (863 Program) (2011AA10A202).

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  • Received Date: November 16, 2015
  • Revised Date: January 14, 2016
  • Available Online: October 31, 2022
  • Published Date: June 27, 2016
    Graphical Abstract
    • Abstract
  • Through two-phase culture, we studied the effects of culture time extension and volume percentages of dibutylphthalate (DBP) on growth and secondary metabolite content in the adventitious roots of Tripterygium wilfordii Hook. f.. Results showed that adventitious root growth increased 1.06-fold when the volume percentage of DBP was 6% and addition of organic solvent was on day 6. Triptolide content in the root was 1.40-fold higher when the volume percentage of DBP was 2% and addition of organic solvent was on day 8. Triptolide yield in the two-phase culture was 1.48-fold higher than that of the control when the volume percentage of DBP was 2% and addition of organic solvent was on day 2. Thus, DBP in the medium was suitable for triptolide accumulation, but alkaloid accumulation was inhibited significantly. The contents of wilforgine and wilforine in the adventitious roots and yields of wilforgine and wilforine per flask were all lower than that of the control, regardless of the concentration of DBP added to the medium or the extension of culture time.
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    • References (20)
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