Pollen Identification on the Plant Origins of Houpo and Four Related Species

WEI Meng; TIAN Liu-Huan; ZHAO Zhi-Li; PAN Meng; TIAN Dai-Zhi; ZHANG Lin-Bi; SEN Lin

doi:10.11913/PSJ.2095-0837.2016.60834

Plant Science Journal > 2016 > 34(6): 834-841. > DOI: 10.11913/PSJ.2095-0837.2016.60834 CSTR: 32231.14.PSJ.2095-0837.2016.60834

WEI Meng, TIAN Liu-Huan, ZHAO Zhi-Li, PAN Meng, TIAN Dai-Zhi, ZHANG Lin-Bi, SEN Lin. Pollen Identification on the Plant Origins of Houpo and Four Related Species[J]. Plant Science Journal, 2016, 34(6): 834-841. DOI: 10.11913/PSJ.2095-0837.2016.60834

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Pollen Identification on the Plant Origins of Houpo and Four Related Species

1. College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China;
2. Key Laboratory of Education Ministry on Traditional Chinese Medicine Resources and Compound Prescription, Wuhan 430065, China

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This work was supported by grants from the National Natural Science Foundation for Young Scientists of China (31500260), the Traditional Chinese Medicine Scientific Research Key Project of Hubei Provincial Health and Family Planning Commission (2012Z-Y27) and the Initial Founding of Key Laboratory of Education Ministry at the Hubei University of Chinese Medicine (5114000914).

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Received Date: August 10, 2016
Revised Date: October 12, 2016
Available Online: October 31, 2022
Published Date: December 27, 2016

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Abstract

Abstract

We observed the basic features of pollens from Magnolia officinalis Rehd. et Wils. and M. officinalis Rehd. et Wils. var. biloba Rehd. et Wils. (two botanical origins of Chinese Materia Medica Houpo) and related species of Magnolia Linnaeus (i.e. M. denudata Desr., M. liliiflora Desr., M. biondii Pampan. and M. grandiflora L.). We employed three methods for treating pollen samples of the six species, observed and compared their pollen morphology, surface ornamentation, and pollen size via scanning electron microscopy. Results indicated that the morphology from the side view, equatorial view, and polar view were quite similar among the studied species. The point ornamentation was small and shallow in Magnolia officinalis, and small but deep in M. officinalis var. biloba. Conversely, pollens from the other four species had obvious deep points and irregular uplifts over the surface. Pollen size ranged from large to small in the following order:Magnolia grandiflora,M. officinalis var. biloba,M. officinalis,M. denudata,M. liliiflora, and M. biondii. Here, the natural drying method outperformed the glutaraldehyde fixing method and the tert-butyl alcohol vacuum freeze drying method in describing the pollen morphology, surface ornamentation, and pollen size. The current research provides novel evidence for the identification of the origins of Chinese Materia Medica Houpo, and benefits further investigation on the lineages of Magnolia officinalis,M. officinalis var. biloba and their related species.
- Scanning electron microscope,
- Magnolia officinalis,
- Magnolia officinalis var. biloba,
- Pollen grain,
- Identification,
- Classification

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References (25)

References

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