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Li Rui-Yuan, Shi Tao-Xiong, Chen Qi-Jiao, Pan Fan, Chen Qing-Fu. Construction of EST-SSR fingerprinting and analysis of genetic diversity of thirty-five registered tartary buckwheat cultivars (Fagopyrum tataricum) in China[J]. Plant Science Journal, 2017, 35(2): 267-275. DOI: 10.11913/PSJ.2095-0837.2017.20267
Citation: Li Rui-Yuan, Shi Tao-Xiong, Chen Qi-Jiao, Pan Fan, Chen Qing-Fu. Construction of EST-SSR fingerprinting and analysis of genetic diversity of thirty-five registered tartary buckwheat cultivars (Fagopyrum tataricum) in China[J]. Plant Science Journal, 2017, 35(2): 267-275. DOI: 10.11913/PSJ.2095-0837.2017.20267
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Construction of EST-SSR fingerprinting and analysis of genetic diversity of thirty-five registered tartary buckwheat cultivars (Fagopyrum tataricum) in China

  • 1. Key Laboratory of Information and Computing Science of Guizhou Province, Guizhou Normal University, Guiyang 550001, China;

  • 2. Research Center of Buckwheat Industry Technology, Guizhou Normal University, Guiyang 550001, China

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This work was supported by grants from the National Natural Science Foundation of China (31460280), Earmarked Fund for China Agriculture Research System (CARS-08-A4), Foundation of Guizhou Natural Science (Qiankehe JZ zi[2014]2011), and Scientific Research Foundation for Doctor of Guizhou Normal University (11904-05032130025, 11904-0514027)

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  • Received Date: June 11, 2016
  • Revised Date: July 20, 2016
  • Available Online: October 31, 2022
  • Published Date: April 27, 2017
    Graphical Abstract
    • Abstract
  • Core primers are important in genetic diversity analysis, cultivar identification, and fingerprinting construction. In this study, 91 pairs of EST-SSR primers were screened using 35 registered tartary buckwheat cultivars, with 50 primers showing polymorphism. Based on the polymorphism information content (PIC) and discrimination power (DP) results, a core set of six SSR primers (SSR9007, SSR6873, SSR7642, SSR2234, SSR6789, and SSR68216) were further selected to construct fingerprinting of the 35 cultivars, with alleles amplified by each pair of primers ranging from 2 to 4 and PIC values ranging from 0.60 to 0.78. The UPGMA cluster analysis showed that the genetic similarity of the 35 cultivars varied from 0.50 to 0.99. The cultivars were divided into four groups based on a genetic similarity of 0.60, with 54.3% remaining clustered together, indicating a close genetic relationship and narrow genetic basis. The genetic relationship was in accordance with their breeding history, but was not affected by their geographic differences.
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    • References (24)
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