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Guo Yan, Zhang Hui, Jian Shu-Guang, Xia Kuai-Fei, Zhang Mei. Construction of a cDNA library of Ipomoea pes-caprae L.and screening for cadmium tolerant genes[J]. Plant Science Journal, 2017, 35(3): 372-378. DOI: 10.11913/PSJ.2095-0837.2017.30372
Citation: Guo Yan, Zhang Hui, Jian Shu-Guang, Xia Kuai-Fei, Zhang Mei. Construction of a cDNA library of Ipomoea pes-caprae L.and screening for cadmium tolerant genes[J]. Plant Science Journal, 2017, 35(3): 372-378. DOI: 10.11913/PSJ.2095-0837.2017.30372
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Construction of a cDNA library of Ipomoea pes-caprae L.and screening for cadmium tolerant genes

  • 1. Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China;

  • 2. University of the Chinese Academy of Sciences, Beijing 100049, China

Funds: 

This work was supported by grants from the National Natural Science Foundation of China (31271702,31570257),Chinese Academy of Sciences Class A Strategic Pilot S&T Special Projects (XDA13020500),National Key Research Projects (2016YFC1403002),and “Twelfth Five-Year” National S&T Projects for Rural Areas (2015BAL04B04).

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  • Received Date: November 01, 2016
  • Available Online: October 31, 2022
  • Published Date: June 27, 2017
    Graphical Abstract
    • Abstract
  • Ipomoea pes-caprae(L.) Sweet,as a coastal beach halophyte with great ecological,ornamental,and medicinal value,has the capacity to enrich heavy metal cadmium and can be used in phytoremediation.We constructed a cDNA library for expression in yeast with tissues of Ipomoea pes-caprae by gateway technology,and then introduced the library plasmids into cadmium-sensitive yeast mutant strain,ycf1△,and screened the library through the FOX gene hunting system based on functional screening in yeast.We obtained two full-length cDNAs of Ipomoea pes-caprae,which could enhance the Cd tolerance of ycf1△.Sequence analyses indicated that these two cDNAs encoded phytochelatin synthase (IpPCS) and metallothionein (IpMT).This research lays a good foundation for further clarifying the molecular mechanism of plants responding to cadmium,and provides a reference cDNA library for functional screening to clone other stress-related genes in Ipomoea pes-caprae.
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    • References (33)
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