Induction of hairy roots of <i>Pyrethrum cinerariifolium</i> Trey. and optimization of culture conditions

Gongye Xiang-Xu; Zhu Hui-Jun; Li Qun; Li Yan; Zhang Xing

doi:10.11913/PSJ.2095-0837.2017.30427

Plant Science Journal > 2017 > 35(3): 427-434. > DOI: 10.11913/PSJ.2095-0837.2017.30427 CSTR: 32231.14.PSJ.2095-0837.2017.30427

Gongye Xiang-Xu, Zhu Hui-Jun, Li Qun, Li Yan, Zhang Xing. Induction of hairy roots of Pyrethrum cinerariifolium Trey. and optimization of culture conditions[J]. Plant Science Journal, 2017, 35(3): 427-434. DOI: 10.11913/PSJ.2095-0837.2017.30427

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Induction of hairy roots of Pyrethrum cinerariifolium Trey. and optimization of culture conditions

1. College of Life Science, Northwest A & F University, Yangling, Shaanxi 712100, China;
2 Research and Development Center of Biorational Pesticide, Northwest A & F University Shaanxi Province Technology and Engineering Center of Biopesticide, Yangling, Shaanxi 712100, China

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This work was supported by grants from the National Natural Science Foundation of China (31272110) and National High Techno-logy Research and Development Program of China (863 Program)(2011AA10A202).

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Received Date: September 07, 2016
Available Online: October 31, 2022
Published Date: June 27, 2017

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Abstract

Inoculation of Pyrethrum cinerariifolium Trey.as an explant was carried out to study the induction of the hairy roots,optimization of culture conditions,detection of pyrethrin in the hairy roots,and determination of biological insecticidal activity.Results showed that acetosyringone promoted hairy root induction of the hypocotyls and cotyledons of P.cinerariifolium;when the concentration of acetosyringone was 150 μmol/L,the inductivities of the hypocotyls and cotyledons were 2.29 and 2.66-fold greater than the contrast control,and after pre-culture for 6 d,the inductivity of the hypocotyls was 2.25-fold greater than the control contrast.The inductivity of A4 was higher than that of ATCC15834.Callus was suitable for inducing hairy roots;after infection,the callus was suitable for inducing hairy roots in the MS medium without hormones,but the addition of 50 mL of MS medium to a 250 mL Erlenmeyer flask was more suitable for the growth of hairy roots.PCR detection showed that the rolB gene in the Ri T-DNA of Agrobacterium rhizogenes was integrated in the genome of the hairy roots.The GCMS detection results showed that the six components of pyrethrin were not detected in the callus,but cinerin Ⅰ,jasmolin Ⅰ,and jasmolin Ⅱ were detected in the hairy roots.The antifeedant effect of the hairy roots on Mythimna separata was obviously better than that of the callus.This study lays the foundation for the production of pyrethrins through tissue culture.
- Pyrethrum cinerariifolium Trey.,
- Agrobacterium rhizogenes,
- Hairy roots,
- Pyrethrins,
- Optimization cultural conditions

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References

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Induction of hairy roots of Pyrethrum cinerariifolium Trey. and optimization of culture conditions

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