Study on the ultrastructure and proteome of oil bodies in <i>Brassica napus</i> L.seeds

He Yu-Qing; Cao Chun-Yan; Shen Wen-Zhong; Huang Dong; Ma Sheng; Wu Yan

doi:10.11913/PSJ.2095-0837.2017.40566

Plant Science Journal > 2017 > 35(4): 566-573. > DOI: 10.11913/PSJ.2095-0837.2017.40566 CSTR: 32231.14.PSJ.2095-0837.2017.40566

He Yu-Qing, Cao Chun-Yan, Shen Wen-Zhong, Huang Dong, Ma Sheng, Wu Yan. Study on the ultrastructure and proteome of oil bodies in Brassica napus L.seeds[J]. Plant Science Journal, 2017, 35(4): 566-573. DOI: 10.11913/PSJ.2095-0837.2017.40566

Citation:

PDF (1774 KB)

Study on the ultrastructure and proteome of oil bodies in Brassica napus L.seeds

College of Life Science, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, China

Funds:

This work was supported by a grant from the National Natural Science Foundation of China (31401416).

More Information

Received Date: October 27, 2016
Available Online: October 31, 2022
Published Date: August 27, 2017

Graphical Abstract

Abstract

Abstract

Rapeseed is one of the most important oil crops in China.The oil content of seeds is directly related to the national economy and people's livelihood.Fatty acids in rapeseeds are stored in the form of oil bodies composed of triacylglycerol (TAG) molecules,which are made of three fatty acid chains joined covalently to a glycerol molecule.We analyzed the ultrastructural characteristics of oil bodies in the seeds of four cultivars that contained varied oil content,including high oil content ‘ZS11’,medium oil content ‘Westar’ and ‘Topas’,and low oil content ‘ZS10’.Results showed that the size and density of the oil bodies in the four cultivars were varied.In the seeds of ‘ZS11’,the density of the oil bodies was higher but the size of the oil bodies was smaller (less than 1 μm in diameter).Interestingly,the density of the oil bodies in the seeds of the medium and low oil content cultivars was lower.Larger sized oil bodies were found in the seeds of the medium oil cultivars,but oil body size in the seeds of the low oil cultivar was diverse.Because oil-body protein composition is related to oil body size and oil content of seeds,total and oil-body protein profiles in the seeds of ‘ZS11’ and ‘Westar’ were explored by two-dimensional electrophoresis.Compared with the total protein profiles,57 differentially expressed proteins were detected in the seeds of both ‘Westar’ and ‘ZS11’ cultivars,indicating at least a two-fold difference in expression levels;in addition,24 and 23 proteins were specifically expressed in ‘Westar’ and ‘ZS11’ seeds,respectively.Compared with the oil-body protein profiles,we observed 52 differentially expressed proteins in the oil bodies of both cultivars,indicating at least a two-fold difference in expression levels;furthermore,we detected 2 and 13 proteins specifically expressed in oil-body proteins of ‘Westar’ and ‘ZS11’ seeds,respectively.Our findings indicate that the ultrastructure and protein composition of the oil bodies differed in different rapeseed cultivars.
- Brassica napus,
- Oil body,
- Ultrastructure,
- Oil body protein,
- Two-dimensional electrophoresis

FullText(HTML)

References (23)

References

[1]	Tzen JT, Huang AH. Surface structure and properties of plant seed oil bodies[J]. J Cell Biol, 1992, 117(2):327-335.
[2]	程红焱, 宋松泉. 种子的贮油细胞器——油体及其蛋白[J]. 植物学通报, 2006, 23(4):418-430. Cheng HY, Song SQ. Seed lipid storage organelles:oil bodys and their proteins[J]. Chinese Bulletin of Botany, 2006, 23(4):418-430.
[3]	Tzen JTC. Integral proteins in plant oil bodies[J]. ISRN Botany, 2012, 2012:173954.
[4]	李玉琴. 不同含油量甘蓝型油菜种子全蛋白及油体蛋白的双向电泳分析[D]. 郑州:郑州大学, 2012.
[5]	Chen JC, Tsai CC, Tzen JT. Cloning and secondary structure analysis of caleosin, a unique calcium-binding protein in oil bodies of plant seeds[J]. Plant Cell Physiol, 1999, 40(10):1079-1086.
[6]	Katavic V, Agrawal GK, Hajduch M, Harris SL, Thelen JJ. Protein and lipid composition analysis of oil bodies from two Brassica napuscultivars[J]. Proteomics, 2006, 6(16):4586-4598.
[7]	Jolivet P, Boulard C, Bellamy A, Valot B, d'Andréa S, et al. Oil body proteins sequentially accumulate throughout seed development in Brassica napus[J]. J Plant Physiol, 2011, 168(17):2015-2020.
[8]	徐春雷. 甘蓝型油菜steroleosin基因的克隆与表达[D]. 武汉:华中农业大学, 2008.
[9]	韦存虚, 钦风凌, 李爱民, 张永泰, 周卫东, 王幼平. 油菜种子油体的观察和大小分析[J]. 中国油料作物学报, 2009, 31(4):445-448. Wei CX, Qin FL, Li AM, Zhang YT, Zhou WD, Wang YP. Oil body observation in seeds of Brassica napusL.[J]. Chinese Journal of Oil Crop Sciences, 2009, 31(4):445-448.
[10]	董劲松. 甘蓝型油菜(Brassica napusL.)油体发育机制及其与含油量相关性研究[D]. 南京:南京农业大学, 2008.
[11]	董劲松, 石东乔, 高建芹, 李成磊, 刘洁, 等. 甘蓝型油菜油体数量及面积之和与含油量的相关性[J]. 植物学报, 2009, 44(1):79-85. Dong JS, Shi DQ, Gao JQ, Li CL, Liu J, et al. Correlation between the quantity and the sum of area of oil bodies and oil content in Rapeseed (Brassica napus)[J]. Chinese Bulletin of Botany, 2009, 44(1):79-85.
[12]	Siloto RM, Findlay K, Lopez-Villalobos A, Yeung EC, Nykiforuk CL, Moloney MM. The accumulation of oleosins determines the size of seed oilbodies in Arabidopsis[J]. Plant Cell, 2006, 18(8):1961-1974.
[13]	Hu Z, Wang X, Zhan G, Liu G, Hua W, Wang H. Unusually large oilbodies are highly correlated with lower oil content in Brassica napus[J]. Plant Cell, 2009, 28(4):541-549.
[14]	Jolivet P, Boulard C, Bellamy A, Larré C, Barre M, et al. Protein composition of oil bodies from mature Brassica napusseeds[J]. Proteomics, 2009, 9(12):3268-3284.
[15]	甘露. 不同含油量甘蓝型油菜品系比较蛋白质组学研究[D]. 武汉:华中科技大学, 2011.
[16]	梁丛敏, 李玉琴, 杨冬之, 臧新, 田保明, 等. 甘蓝型油菜种子油体蛋白提取及双向电泳分析[J]. 南方农业学报, 2013, 44(11):1776-1780. Liang CM, Li YQ, Yang DZ, Zang X, Tian BM, et al. 2D-polyacrylamide gel electrophoresis analysis and extraction of oil body protein in seeds of Brassica napus[J]. Journal of Southern Agriculture, 2013, 44(11):1776-1780.
[17]	Gan L, Zhang CY, Wang XD, Wang H, Long Y, et al. Proteomic and comparative genomic analysis of two Brassica napuslines differing in oil content[J]. J Proteome Res, 2013, 12(11):4965-4978.
[18]	官春云. 加拿大油菜品种的演变及现状[J]. 中国油料作物学报, 1994, 16(4):74-79. Guan CY. Evolution and status of rape varieties in Canada[J]. Chinese Journal of Oil Crop Sciences, 1994, 16(4):74-79.
[19]	Elahi N, Duncan RW, Stasolla C. Modification of oil and glucosinolate content in canola seeds with altered expression of Brassica napusLEAFY COTYLEDON1[J]. Plant Physiol Biochem, 2016, 100:52-63.
[20]	Dawood M, Sadak M, Reyad B, El-Sayed A, El-Gayar S. Changes in chemical composition during germination of some canola varieties changes in oil content and fatty acid composition[J]. Sci Agri, 2013, 2(3):77-82.
[21]	Mekki B. Yield and quality traits of some canola varieties grown in newly reclaimed sandy soils in Egypt[J]. World Appl Sci J, 2013, 25(2):258-263.
[22]	傅丽霞, 瞿波. 不同含油量油菜种子子叶贮藏细胞内脂体和蛋白体超微结构的研究[J]. 华中农业大学学报, 1993, 12(6):556-560. Fu LX, Qu B. Studies on the ultrastructure of oily bodies and protein bodies in the cotyledon storage cell of mature seeds in rapeseed[J]. Journal of Huazhong Agricultural Universiy, 1993, 12(6):556-560.
[23]	Boulard C, Bardet M, Chardot T, Dubreucq B, Gromova M, et al. The structural organization of seed oil bodies could explain the contrasted oil extractability observed in two rapeseed genotypes[J]. Planta, 2015, 242(1):53-68.

[1]	Zhou Si-Yu, Wang Yong-Qing. Relationship between embryonic callus induction from leaves and endogenous hormone content in loquat (Eriobotrya japonica Lindl.)[J]. Plant Science Journal, 2017, 35(1): 99-106. DOI: 10.11913/PSJ.2095-0837.2017.10099
[2]	WEN Jin-Yan, ZHANG Shuo, GAO Su-Ping, LEI Ting, LUO Liang-Xu, LUO Yan. Development Process of Pistils and Stamens and Effect of Endogenous Hormone Content on Style Growth in Plumbago auriculata Lam. with Distyly[J]. Plant Science Journal, 2016, 34(2): 263-270. DOI: 10.11913/PSJ.2095-0837.2016.20263
[3]	MA Li-Yuan, QI Guo-Hui, LI Bao-Guo, GUO Su-Ping, ZHANG Xue-Mei, SHI Li-Li, DONG Li-Xin, LIU Xi-Xing. Content of Endogenous Phytohormones and Isoenzymes of Peroxidase in Male and Female Pistacia chinensis Plants Bunge Leaves[J]. Plant Science Journal, 2013, 31(3): 297-303. DOI: 10.3724/SP.J.1142.2013.30297
[4]	SONG Li-Hong, CAO Bang-Hua. Studies on Activities of Indoleacetic Acid Oxidase, Polyphenol Oxidase and Peroxidase in Cuttings of Broussonetia papyrifera during Rooting Process[J]. Plant Science Journal, 2005, 23(4): 347-350.
[5]	JIANG Jie-Zeng, QIU Jie-Juan, HAN Xiu-Qin, CAO Bei-Sheng, ZHU Qing-Sen. Changes of Endogenous Hormone Contents of Different Partsduring Development of Water Bamboo(Zizania latifolia)[J]. Plant Science Journal, 2004, 22(3): 245-250.
[6]	CHEN Li-Song, LIU Xin-Hui. Changes in Peroxidase and IAA Oxidase Activities in Litchi Leaves under Water Stress[J]. Plant Science Journal, 2002, 20(2): 131-136.
[7]	WANG Ming-Gang, LUO Mao-Chun, ZHANG Xiang-Hong, SHEN Ming-Shan, CHEN Mu-Chuan. Effects of Low Energy Carbon Ion and Nitrogen Ion Implantation on Germinant Rate, Growth Weight and Peroxidase of Stevia rebaudiana Bertoni[J]. Plant Science Journal, 2001, 19(2): 143-148.
[8]	Xie Kaibin, Shi Guoxin, Chen Guoxiang, Chang Fuchen, Ding Xiaoyu. THE EFFECTS OF Hg²⁺ POLLUTION ON ACTIVITY OF PEROXIDASE IN ROOTS OF EURYALE FEROX SALISB AND TRAPA BICORNIS OSBECK[J]. Plant Science Journal, 2000, 18(1): 70-72.
[9]	He Xiaowei, Chen Kecheng, Xiao Yihua. EFFECT OF RED OR FAR-RED LIGHT AND PLANT HORMONE ON THE GROWTH AND ACTIVITY OF CAT, POX IN SEEDING OF RICE[J]. Plant Science Journal, 1993, 11(2): 125-129.
[10]	Hou Songsheng, Li Xinmin, Shang Tinglan, Wu Yulan, Li Honglin. STUDIES ON PHYSIOLOGICAL AND BIOCHEMICAL CHARACTER IN SUSPENSION CULTURE OF BERBERIS JULIANAE SCHNEID CELLS Ⅰ.Relationship between cell growth and changes in the conductivity and peroxidase activity of cultured medium[J]. Plant Science Journal, 1988, 6(2): 129-132.

Cited By

Get Citation

PDF

XML

Article views (1149) PDF downloads (1303)

Turn off MathJax

Article Contents

Abstract

References

Study on the ultrastructure and proteome of oil bodies in Brassica napus L.seeds

Abstract

References

Related Articles

Catalog

Study on the ultrastructure and proteome of oil bodies in Brassica napus L.seeds

Abstract

References

Related Articles

Catalog

Export File

Citation

Format

Content