Optimization of the extraction process of podophyllotoxin from <i>Diphylleia sinensis</i> H. L. Li by response surface methodology

Qiu Zi-Dong; Jin Qing-Hao; Guo Ming-Quan

doi:10.11913/PSJ.2095-0837.2017.50774

Plant Science Journal > 2017 > 35(5): 774-782. > DOI: 10.11913/PSJ.2095-0837.2017.50774 CSTR: 32231.14.PSJ.2095-0837.2017.50774

Qiu Zi-Dong, Jin Qing-Hao, Guo Ming-Quan. Optimization of the extraction process of podophyllotoxin from Diphylleia sinensis H. L. Li by response surface methodology[J]. Plant Science Journal, 2017, 35(5): 774-782. DOI: 10.11913/PSJ.2095-0837.2017.50774

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Optimization of the extraction process of podophyllotoxin from Diphylleia sinensis H. L. Li by response surface methodology

1. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China;
3. National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
4. Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, China

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This work was supported by grants from the National Natural Science Foundation of China (81673580) and the "One Hundred Talents Project" from the Chinese Academy of Sciences (29Y429291a0129).

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Received Date: December 28, 2016
Available Online: October 31, 2022
Published Date: October 27, 2017

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Abstract

Abstract

Podophyllotoxin (PPT) is a frequently used clinical natural drug. In this study, we aimed to optimize the extraction process of PPT from Diphylleia sinensis H. L. Li by response surface methodology (RSM). Based on single factor experiments, three important factors, that is, ethanol concentration, extraction temperature, and extraction time, were selected for further optimization with three-factor/three-level Box-Behnken design. Based on the extraction rate of PPT, the optimal conditions were:ethanol concentration of 48.18%, extraction temperature of 62.66℃, and extraction time of 16.15 h. Under these optimal conditions, PPT yield could reach 7.636%, with the confirmatory experiment reaching 7.54%. Thus, there was no significant difference compared with the theoretical value, verifying the optimized method with RSM in this study.
- Diphylleia sinensis,
- Podophyllotoxin,
- Response surface methodology,
- Extraction process

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References (23)

References

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Optimization of the extraction process of podophyllotoxin from Diphylleia sinensis H. L. Li by response surface methodology

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