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Duan Yong-Hong, Zhang Xu, Liu Qing-Shan, Sun Yi. Construction of methylation linkage groups LGC and LGD with MSAP and SSR markers in Sorghum bicolor (L.) Moench[J]. Plant Science Journal, 2018, 36(2): 237-244. DOI: 10.11913/PSJ.2095-0837.2018.20237
Citation: Duan Yong-Hong, Zhang Xu, Liu Qing-Shan, Sun Yi. Construction of methylation linkage groups LGC and LGD with MSAP and SSR markers in Sorghum bicolor (L.) Moench[J]. Plant Science Journal, 2018, 36(2): 237-244. DOI: 10.11913/PSJ.2095-0837.2018.20237
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Construction of methylation linkage groups LGC and LGD with MSAP and SSR markers in Sorghum bicolor (L.) Moench

  • 1. Sorghum Institute Shanxi Academy of Agricultural Sciences, Key Laboratory of Sorghum Genetic and Germplasm Innovation of Shanxi Province, Jinzhong, Shanxi 030600, China;

  • 2. College of Agronomy, Shanxi Agricultural University, Taigu, Shanxi 030801, China;

  • 3. Biotechnology Research Center, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China;

  • 4. Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan 030031, China

Funds: 

This work was supported by grants from the Key Laboratory Innovation of Shanxi Province (2015K-03), Natural Science Foundation of Shanxi Province (201701D121105), and Supporting Agriculture Project from Department of Finance of Shanxi Province (2015TGSF-10).

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  • Received Date: August 06, 2017
  • Available Online: October 31, 2022
  • Published Date: April 27, 2018
    Graphical Abstract
    • Abstract
  • The F2 population derived from crossing sorghum cultivars ‘B2V4’ and ‘1383-2’ was used as research material, with the SSR and MSAP markers used to detect the sorghum genome differences and construct methylation linkage groups. Results showed that the methylation linkage group LGC covered 44.3 cM, of which 23 loci were MSAP markers and three loci were SSR markers; the methylation linkage group LGD covered 46.2 cM, of which eight loci were MSAP markers and four loci were SSR markers. The methylation sites on linkage group LGC were from EcoRⅠ/MspⅠ enzyme digestion, but the methylation sites on linkage group LGD were from EcoRⅠ/MspⅠ and EcoRⅠ/HpaⅡ enzyme digestions. A dense methylation region near Xtxp 69 in LGC was identified. The MSAP marker rapidly detected genomic methylation differences and was able to construct a methylation linkage group in plants.
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    • References (22)
  • [1]
    Kawashima T, Berger F. Epigentic reprogramming in plant sexual reproduction[J]. Nat Rev Genet, 2014, 15(9):613-624.
    [2]
    邓莹, 高乐旋, 朱珠, 杨继. 不同水陆环境中喜旱莲子草甲基化调控因子表达水平和差异表达基因启动子区甲基化状态分析[J]. 植物科学学报, 2014, 32(5):475-486.

    Deng Y, Gao LX, Zhu Z, Yang J. Differential expression of DNA methylation regulating factors and dynamic methy-lation patterns of Alternanthera philoxeroides under diffe-rent water treatments[J]. Plant Science Journal, 2014, 32(5):475-486.
    [3]
    Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, et al. AFLP:a new technique for DNA fingerprinting[J]. Nucleic Acids Res, 1995, 23(21):4407-4414.
    [4]
    崔国鹏, 王圣洁, 覃瑞, 刘虹, 龚汉雨, 等. 极性生长的细胞中胞嘧啶甲基化的动态变化及其对GABA信号的响应[J]. 植物科学学报, 2015, 33(4):521-529.

    Cui GP, Wang SJ, Qin R, Liu H, Gong HY, et al. Dyna-mic changes of methylcytosine in polarized cell growth and their response to GABA signals[J]. Plant Science Journal, 2015, 33(4):521-529.
    [5]
    何庆元, 向仕华, 李正鹏, 王松华, 祝嫦巍, 等. 利用MSAP分析硫化氢对盐胁迫大豆甲基化的影响[J]. 中国油料作物学报, 2016, 38(2):195-201.

    He QY, Xiang SH, Li ZP, Wang SH, Zhu CW, et al. Methylation of soybean (Glycine max) under salt stress by H2S treatment with MSAP[J]. Chinese Journal of Oil Crop Science, 2016, 38(2):195-201.
    [6]
    吴春太, 班硕, 黎瑜, 曾日中. 橡胶树MSAP反应体系优化及其不同开割高度单株DNA甲基化分析[J]. 植物研究, 2016, 36(6):860-869.

    Wu CT, Ban S, Li Y, Zeng RZ. Optimization of MSAP analysis system for Hevea brasiliensis and its application in plant of different heights of opening for tapping[J]. Bulletin of Botanical Research, 2016, 36(6):860-869.
    [7]
    何宇宁, 徐仲瑞, 熊治廷. 铜胁迫对不同抗性种群海州香薷酸性转化酶基因启动子甲基化的影响[J]. 植物科学学报, 2017, 35(4):497-505.

    He YN, Xu ZR, Xiong ZT. DNA methylation patters of acid invertase gene promoters from Cu-tolerant and no-tolerant populations of Elsholtzia haichowensis under copper stress[J]. Plant Science Journal, 2017, 35(4):497-505.
    [8]
    杨丽娟, 敖游, 王丹, 白琰, 赵洁宇, 郭长虹. 小麦杀配子染色体单体附加系CS-2C的DNA甲基化变异分析[J]. 核农学报, 2016, 30(7):1281-1287.

    Yang LJ, Ao Y, Wang D, Bai Y, Zhao JY, Guo CH. Variation analysis of DNA methylation of gametocidal chromosome 2C[J]. Journal of Nuclear Agricultural Science, 2016, 30(7):1281-1287.
    [9]
    李廷春, 杨华应, 张玮, 周应兵, 陈洪俭. 紫玉米籽粒呈色过程中DNA甲基化变化的MSAP分析[J]. 核农学报, 2014, 28(11):1978-1984.

    Li TC, Yang HY, Zhang W, Zhou YB, Chen HJ. MASP analysis of DNA methylation variations during coloring process for purple corn seed[J]. Journal of Nuclear Agricultural Science, 2014, 28(11):1978-1984.
    [10]
    Paterson AH, Bowers JE, Bruggmann R, Dubchak I, Grimwood J, et al. The Sorghum bicolor genome and the diversification of grasses[J]. Nature, 2009, 457(29):551-556.
    [11]
    Duan YH, Qian J, Sun Y, Yi ZB, Yan M. Construction of methylation linkage map based on MSAP and SSR markers in Sorghum bicolor (L.)[J]. IUBMB Life, 2009, 61(16):663-669.
    [12]
    Xu WW, Subudhi PK, Crasta OR, Rosenow DT, Mullet JE, Nguyen HT. Molecular mapping of QTLs conferring stay-green in grain sorghum (Sorghum bicolor L. Moench)[J]. Genome, 2000, 43:461-469.
    [13]
    Tao YZ, Jordan DR, Henzell DG,Mcintyre CL. Identification of genomic regions for rust resistance in sorghum[J]. Euphytica, 1998, 103(3):287-292.
    [14]
    Katsar CS, Paterson AH, Teetes GL, Peterson GC. Molecular analysis of sorghum resistance to the greenbug(Homoptera:Aphididae)[J]. Plant Resistance, 2002, 95(2):448-457.
    [15]
    王瑞, 王金胜, 张福耀, 程庆军, 田承华, 凌亮. 1970s-2000s中国高粱杂交种亲本遗传距离演变的SSR分析[J]. 中国农业科学, 2015, 48(3):415-425.

    Wang R, Wang JS, Zhang FY, Cheng QJ, Tian CH, Ling L. Evolution of genetic distance between parental lines of Chinese sorghum hybrids from 1970s-2000s based on SSR analysis[J]. Scientia Agricultura Sinica, 2015, 48(3):415-425.
    [16]
    高建明, 夏卜咸, 杨洪, 曲荣桂, 桂枝, 等. 4种高粱基因组DNA快速提取方法的比较[J]. 安徽农业科学, 2011, 39(23):13942-13943, 13946.

    Gao JM, Xia BX, Yang H, Qu RG, Gu Z, et al. Comparative study on four methods for quick extraction of sorghum genomic DNA[J]. Journal of Anhui Agricultura Sciences, 2011, 39(23):13942-13943, 13946.
    [17]
    Xiong LZ, Xu CG, Maroof MA, Zhang QF. Patterns of cytosine methylation in an elite rice hybrid and its parental lines detected by a methylation-sensitive amplification poly-morphism technique[J]. Mol Gen Genet, 1999, 26:439-446.
    [18]
    Klein RR, Rodriguez-Herrera R, Schlueter JA, Klein PE, Yu ZH, Rooney WL. Identification of genomic regions that affect grain mold incidence and other traits of agronomic importance in sorghum[J]. Theor and Appl Genet, 2001, 102:307-319.
    [19]
    Haussmann BIG, Mahalakshmi V, Reddr BVS, Seetharama N, Hash CT, Geiger HH. QTL mapping of stay-green in two sorghum recombinant inbred populations[J]. Theor and Appl Genet, 2002, 106:133-142.
    [20]
    卞云龙, 邓德祥, 王益军, 才宏伟. 基于AFLP和SSR标记的高粱分子遗传连锁图构建[J]. 分子植物育种, 2007, 5(5):661-666.

    Bian YL, Deng DX, Wang YJ, Cai HW. Construction of genetic linkage map based on AFLP and SSR markers in sorghum[J]. Molecular Plant Breeding, 2007, 5(5):661-666.
    [21]
    段永红. 高粱甲基化和微卫星标记遗传连锁图的构建[D]. 太原:山西大学, 2010.
    [22]
    段永红, 王铭, 孙毅, 杨武德. 高粱甲基化连锁群A、B的构建及甲基化位点、甲基化模式的分析[J]. 中国农业科学, 2012, 45(18):3699-3708.

    Duan YH, Wang M, Sun Y, Yang WD. Construction of methylation linkage groups A and B in sorghum with MSAP and SSR markers and analysis of methylation sites and patterns[J]. Scientia Agricultura Sinica, 2012, 45(18):3699-3708.
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