Study on the adventitious root culture of <em>Tripterygium wilfordii</em> under shaking culture conditions

Li Yan; Zhao Lei; Wen Peng-Fei; Ge Fu-Hua; Zhang Xing

doi:10.11913/PSJ.2095-0837.2019.10093

Plant Science Journal > 2019 > 37(1): 93-100. > DOI: 10.11913/PSJ.2095-0837.2019.10093 CSTR: 32231.14.PSJ.2095-0837.2019.10093

Li Yan, Zhao Lei, Wen Peng-Fei, Ge Fu-Hua, Zhang Xing. Study on the adventitious root culture of Tripterygium wilfordii under shaking culture conditions[J]. Plant Science Journal, 2019, 37(1): 93-100. DOI: 10.11913/PSJ.2095-0837.2019.10093

Citation:

PDF (867 KB)

Study on the adventitious root culture of Tripterygium wilfordii under shaking culture conditions

Li Yan^1,2, ,,
Zhao Lei^1,2,
Wen Peng-Fei^1,2,
Ge Fu-Hua¹,
Zhang Xing²

1.
College of Life Science, Northwest A & F University, Yangling, Shaanxi 712100, China
2. Shaanxi Province Technology and Engineering Center of Biopesticide, Yangling, Shaanxi 712100, China

Funds: This work was supported by grants from the National Natural Science Foundation of China (31272110) and National High Techno-logy Research and Development Program of China (863 Program) (2011AA10A202)

More Information

Received Date: July 08, 2018
Revised Date: August 31, 2018
Published Date: February 27, 2019

Graphical Abstract

Abstract

Abstract

Tripterygium wilfordii Hook. f., adventitious roots were used in vitro to study the effects of inoculation density, liquid medium volume per flask, step-by-step amplification, defoaming agent, macroporous adsorption (MA) resin type, and concentrations of triptolide, wilforgine, and wilforine in roots and medium on adventitious root growth after shake-flask culture. Results showed that an inoculation density of 15 g/L was suitable for the subculture of adventitious roots and accumulation of secondary metabolite. The medium utilization rate was the highest in a 250-mL flask with 100 mL of liquid medium. Adventitious root growth declined slightly with the increase in flask volume. In a 5-L bottle, the adventitious root growth and triptolide, wilforgine, and wilforine concentrations were 91.6%, 91.8%, 91.7%, and 96.9%, respectively, compared to the control， XAD-7 resin markedly enhanced adventitious root growth and secondary metabolite content among the six kinds of MA resin. The adventitious root growth and triptolide, wilforgine, and wilforine content increased 1.2-, 2.9-, 2.4-, and 2.2-fold, respectively, compared to the control when XAD-7 resin was at 0.5 g/bottle. Adventitious root growth and secondary metabolite content all decreased at differing degrees when the defoaming agent was added to the medium. Adventitious root growth and triptolide, wilforgine, and wilforine contents were 85%, 78%, 64%, and 87%, respectively, compared to the control when LX-603 was added to the medium; however, foam was markedly inhibited during the culturing process. This study demonstrated that step-by-step amplification was a feasible approach for adventitious root culture. Furthermore, this research provides the basis for the scale-up culture of T. wilfordii adventitious roots using a bioreactor.
- Tripterygium wilfordii,
- Adventitious roots,
- Shaking culture,
- Secondary metabolite

FullText(HTML)

References (33)

References

[1]	陈同素. 国产杀虫药剂雷公藤调查报告[J]. 中华农学会报,1933,125:79-82.
[2]	Swingle WT,Haller HL,Siegler EH,Swingle MC. A Chinese insecticidal plant,Tripterygium wilfordii,introduced into the United States[J]. Science,1941,93(2403):60-61.
[3]	Tao X,Cai JJ,Lipsky P. The identity of immunosuppressive components of the ethyl acetate extract and chloroform methanol extract (T2) of Tripterygium wilfordii Hook. F. [J]. Journal of Pharmacology and Experimental Therapeutics,1995,272(3):1305-1316.
[4]	岗艳云,张正行. 雷公藤及其单体的药理作用研究进展[J]. 中国药科大学学报,1995,26(4):252-256.
[5]	Liu QY. Triptolide and its expanding multiple pharmacolo-gical functions[J]. International immunopharmacology,2011,11(3):377-383.
[6]	Lu Y,Zhang Y,Li L,Feng XJ,Ding S,Zheng W,Li JX,Shen PP. TAB1:A target of triptolide in macrophages[J]. Chemistry & Biology,2014,21:246-256.
[7]	周琳,马志卿,冯俊涛,李修伟,张兴. 雷公藤生物碱制品对小菜蛾和菜青虫的控制效果[J]. 西北农林科技大学学报:自然科学版,2006,34(12):169-173. Zhou L,Ma ZQ,Feng JT,Li XW,Zhang X. Control efficacy of alkaloid products from Tripterygium wilfordii Hook against Plutella xylostella and Pieris rape[J]. Journal of Northwest Sci-Tech University of Agriculture and Forestry:Natural Science Edition,2006,34(12):169-173.
[8]	周琳,李俊领,刘向阳,马志卿,冯俊涛,张兴. 雷公藤总生物碱对粘虫幼虫神经系统酶活性及神经递质含量的影响[J]. 昆虫学报,2015,58(8):856-863. Zhou L,Li JL,Liu XY,Ma ZQ,Feng JT,Zhang X. Effects of total alkaloids from Tripterygium wilfordiion the activities of neural enzymes and contents of neurotransmitters in larval Mythimna separata[J]. Acta Entomologica Sinica,2015,58(8):856-863.
[9]	Luo DQ,Zhang X,Tian X,Liu JK. Insecticidal compounds from Tripterygium wilfordiiactive against Mythimna separata[J]. Zeitschrift fur Naturforschung. C,A Journal of Biosciences,2004,59(5-6):421-426.
[10]	黄文华,张蕊,郭宝林,斯金平. 雷公藤药材总生物碱含量及影响因素的研究[J]. 中国中药杂志,2008,33(1):15-18.Huang WH,Zhang R,Guo BL,Si JP. Study on total alkaloids content and its influential factors in medicinal mate rials of Tripterygium[J]. China Journal of Chinese Materia Medica,2008,33(1):15-18.
[11]	张璟,陈蒙蒙,蒲时,祝传书,张兴. 雷公藤中雷公藤甲素、雷公藤吉碱和次碱的高效液相色谱-电喷雾串联质谱分析方法[J]. 农药学学报,2018,20(2):197-203. Zhang J,Chen MM,Pu S,Zhu CS,Zhang X. Determination of triptolide,wilforgine and wilforine in Tripterygium wilfordiiby high performance liquid chromatography-electrospray ionization tandem mass spectrometry[J]. Chinese Journal of Pesticide Science,2018,20(2):197-203.
[12]	Brinker AM,Raskin I. Determination of triptolide in root extracts of Tripterygium wilfordiiby solid-phase extraction and reverse-phase high-performance liquid chromatography[J]. J Chromatogr A,2005,1070(1-2):65-70.
[13]	Ni L,Ma J,Li CJ,Li L,Guo JM,Yuan SP,Hou Q,Guo Y,Zhang DM. Novel rearranged and highly oxygenated abietane diterpenoids from the leaves of Tripterygium wilfordii[J]. Tetrahedron Lett,2015,56(10):1239-1243.
[14]	洪伟,李键,吴承祯,洪滔,范海兰,唐佳栋,陈灿.雷公藤栽培及利用研究综述[J].福建林学院学报,2007,27(1):92-96. Hong W,Li J,Wu CZ,Hong T,Fan HL,Tang JD,Chen C. Research summary of cultivation and utilization of Tripterygium wilfordii[J]. Journal of Fujian College ofForestry,2007,27(1):92-96.
[15]	李琰,冯俊涛,史晓燕,吴勇延,张兴.雷公藤组培产物中雷公藤甲素和总生物碱含量的测定[J].农药学学报,2009,11(3):367-372. Li Y,Feng JT,Shi XY,Wu YY,Zhang X. Study on the determination of triptolide and total alkaloids in Tripterygium wilfordiiHook. f. cultures[J]. Chinese Journal of Pesticide Science,2009,11(3):367-372.
[16]	张兴,李琰,冯俊涛,王智辉,王永宏. 一种由雷公藤悬浮细胞诱导不定根的方法:ZL200810017697[P].2008-08-27[2018-07-09].
[17]	刘跃,张红蕊,赵林,刘新利.消泡剂在发酵工业中的应用[J]. 齐鲁工业大学学报:自然科学版,2014,28(2):37-39. Liu Y,Zhang HR,Zhao L,Liu XL. Application of antifoams in fermentation processes[J]. Journal of Qilu University of Technology:Natural Science Edition,2014,28(2):37-39.
[18]	梁小玲,黄伟敏,黄区,李俏,周建湘.泡沫对食品发酵的影响及控制[J]. 现代食品,2016(14):22-23.Liang XL,Huang WM,Huang Q,Li Q,Zhou JX. The influence and control of foam in food fermentation[J]. Modern Food,2016(14):22-23.
[19]	Miao GP,Zhu CS,Yang YQ,Feng MX,Ma ZQ,Feng JT,Zhang X. Elicitation and in situ adsorption enhanced secondary metabolites production of Tripterygium wilfordiiHook. f. adventitious root fragment liquid cultures in shake flask and a modified bubble column bioreactor[J]. Bioproc Biosyst Eng,2014,37(4):641-650.
[20]	Yan Q,Hu ZD,Tan RX,Wu JY. Efficient production and recovery of diterpenoid tanshinones in Salvia miltiorrhizahairy root cultures with in situ adsorption,elicitation and semi-continuous operation[J]. J Biotechnol,2005,119(4):416-424.
[21]	Miao GP,Zhu CS,Feng JT,Han J,Song XW,Zhang X. Aggregate cell suspension cultures of Tripterygium wilfordiiHook. f. for triptolide,wilforgine,and wilforine production[J]. Plant Cell Tiss Org,2013,112(1):109-116.
[22]	祝传书,刘艳,陈蒙蒙,蒲时,崔蕾,李琰,张兴.雷公藤转录因子TwWRKY1基因的克隆与表达分析[J].农业生物技术学报,2018,26(4):595-605. Zhu CS,Liu Y,Chen MM,Pu S,Cui L,Li Y,Zhang X. Cloning and expression analysis of transcription factor TwWRKY1gene in Tripterygium wilfordii[J]. Journal of Agricultural Biotechnology,2018,26(4):595-605.
[23]	徐步青,李振中,张俊,王芬,刘幸佳,崔永一.不同培养条件对铁皮石斛类原球茎生物反应器培养的影响[J]. 中草药,2014,45(5):709-713.Xu BQ,Li ZZ,Zhang J,Wang F,Liu XJ,Cui YY. Effect of different culture conditions on protocorm-like bodies of Dendrobium candidumin bioreactor culture[J]. Chinese Traditional and Herbal Drugs,2014,45(5):709-713.
[24]	姚睿,朴炫春,李铁军,邵春绘,廉美兰.利用气升式生物反应器培养铁皮石斛原球茎[J].中国中药杂志,2012,37(24):3763-3767.Yao R,Piao XC,Li TJ,Shao CH,Lian ML. Cultivation of protocorms of Dendrobium candidumin air-lift bioreactors[J]. China Journal of Chinese Materia Medica,2012,37(24):3763-3767.
[25]	Lee CWT,Shuler ML. The effect of inoculum density and conditioned medium on the production of ajmalicine and catharanthine from immobilized Catharanthus roseuscells[J]. Biotechnol Bioeng,2000,67(1):61-71.
[26]	姜银姬,朴炫春,李贺,王洪秋,廉美兰. 几种因素对生物反应器培养东北刺人参不定根中皂苷积累的影响[J]. 林业工程学报,2016,1(3):68-72.Jiang YJ,Piao XC,Li H,Wang HQ,Lian ML. Effect of several factors on saponin-accumulation in adventitious roots of Oplopanax elatuscultured with bioreactor culture[J]. Journal of Forestry Engineering,2016,1(3):68-72.
[27]	陈书安,王晓东,袁晓凡,赵兵,王玉春.藏红花细胞悬浮培养体系的建立及优化[J].生物技术通报,2000,(7):157-160.Chen SA,Wang XD,Yuan XF,Zhao B,Wang YC. Establishment and optimization of Crocus sativusL. [J]. Cell Suspension Culture System Biotechnology Bulletin,2000,(7):157-160.
[28]	李琰. 雷公藤组织培养生产次生代谢产物及其代谢调控研究[D]. 杨凌:西北农林科技大学,2008.Li Y. Study on secondary metabolites production and metabolism regulation of Tripterygium wilfordiiHook. f. tissue culture[D]. Northwest Sci-Tech University of Agriculture and Forestry,2008.
[29]	董漪,吴虹. 大孔吸附树脂提取及纯化中药研究进展[J]. 安徽中医学院学报,2012,31(3):76-78.Dong Y,Wu H. Research progress on Chinese traditional medicine extraction and purification by macroporous adsorption resin[J]. Journal of Anhui Traditional Chinese Medical College,2012,31(3):76-78.
[30]	Asada M,Shuler ML. Stimulation of ajmalicine production and excretion from Catharanthus roseus:effects of adsorption in situ,elicitors and alginate immobilization[J]. Appl Microbiol Biot,1989,30(5):475-481.
[31]	Gao MB,Zhang W,Ruan C. Significantly improved taxu yunnanine C production in cell suspension cultures of Taxus chinensisby process intensification of repeated elici tation,sucrose feeding,and in situ adsorption[J]. World J Microb Biot,2011,27(10):2271-2279.
[32]	吴德智,蔡佳,管咏梅,王森,陈丽华,欧阳胜. 不同型号大孔树脂对雷公藤提取物主要成分富集作用考察[J]. 中国实验方剂学杂志,2012,18(17):14-16.Wu DZ,Cai J,Guan YM,Wang S,Chen LH,Ouyang S. Investigation of enrichment effect for main ingredients from extract of Tripterygium wilfordiiby different types of macroporous resin[J]. Chinese Journal of Experimental Traditional Medical Formulae,2012,18(17):14-16.
[33]	Robins RJ,Rhodes MJC. The stimulation of anthraquinone production by Cinchona ledgerianacultures with polymeric adsorbents[J]. Appl Microbiol Biot,1986,24(1):35-41.

[1]	Peng Li-Ping, Cheng Fang-Yun, Zhong Yuan, Xu Xing-Xing, Xian Hong-Li. Phenotypic variation in cultivar populations of Paeonia ostii T. Hong et J. X. Zhang[J]. Plant Science Journal, 2018, 36(2): 170-180. DOI: 10.11913/PSJ.2095-0837.2018.20170
[2]	ZHANG Wen-Liu, GAO Jiang-Yun, LIU Qiang. Habenaria chlorina, a Newly Recorded Species of Orchidaceae from China(英文稿)[J]. Plant Science Journal, 2016, 34(1): 18-20. DOI: 10.11913/PSJ.2095-0837.2016.10018
[3]	LIAO Zhen-Ni, HUANG Qing, CHENG Qi-Ming, YU Xiao-Ying, LI Xiao-Peng, LIU En-Xue. Effect of Plant Age on Botanical Characteristics and Chemical Composition of Essential Oil from Lavandin[J]. Plant Science Journal, 2014, 32(5): 517-521. DOI: 10.11913/PSJ.2095-0837.2014.50517
[4]	LIU Wei, ZHANG Lin, ZHOU Jie, SHI Guo-Yu, LIU Jian-Hua, WANG Xiao. Comparison of Active Constituents at Different Growth Stages in Salvia miltiorrhiza Bge. and Salvia miltiorrhiza Bge. f. alba[J]. Plant Science Journal, 2013, 31(6): 596-602. DOI: 10.3724/SP.J.1142.2013.60596
[5]	WANG Yi, WANG Yan. Habenaria anomaliflora,a New Record of Orchidaceae from China[J]. Plant Science Journal, 2010, 28(6): 696-697.
[6]	DAI Xi-Ling, WANG Quan-Xi, ZHU Rui-Liang, CAO Jian-Guo. Structure and Development of the Sporoderm in Coniogramme japonica (Thunb.) Diels (Hemionitidaceae)[J]. Plant Science Journal, 2010, 28(2): 119-125.
[7]	MU Hong-Ping, YE Wan-Hui, CHEN Yi-Zhu, ZHU Jian-Ling, CAO Hong-Lin. Photosynthesis and Growth of Ardisia crenata and Ardisia punctata under Different Phosphorus Nutrition Levels[J]. Plant Science Journal, 2008, 26(5): 514-519.
[8]	CHEN Li-Jun, RAO Wen-Hui. The Occurrence of Habenaria leptoloba(Orchidaceae) in Guangdong[J]. Plant Science Journal, 2008, 26(3): 255-258.
[9]	LIU Shi-Biao, HU Zheng-Hai. Effects of Shading Treatment on the Leaf Morphology, Structure and Photosynthetic Characteristics of Gynostemma pentaphyllum[J]. Plant Science Journal, 2004, 22(4): 339-344.
[10]	LIU Zhong-Jian, CHEN Sing-Chi. Cymbidium aestivum,A New Species of Orchidaceae from Yunnan[J]. Plant Science Journal, 2004, 22(4): 323-325.

Cited By

Get Citation

PDF

XML

Article views (536) PDF downloads (434)

Turn off MathJax

Article Contents

Abstract

References

Study on the adventitious root culture of Tripterygium wilfordii under shaking culture conditions

Abstract

References

Related Articles

Catalog

Study on the adventitious root culture of Tripterygium wilfordii under shaking culture conditions

Abstract

References

Related Articles

Catalog

Export File

Citation

Format

Content