Estimation of genome size from eight populations of four species of <i>Soroseris</i> Stebbins by flow cytometry

Yang Hui-Xian; Rao Pei-Yu; Hu Jing-Jing; Ou Xiao-Kun; Li Zhi-Min

doi:10.11913/PSJ.2095-0837.2019.60775

Plant Science Journal > 2019 > 37(6): 775-780. > DOI: 10.11913/PSJ.2095-0837.2019.60775 CSTR: 32231.14.PSJ.2095-0837.2019.60775

Yang Hui-Xian, Rao Pei-Yu, Hu Jing-Jing, Ou Xiao-Kun, Li Zhi-Min. Estimation of genome size from eight populations of four species of Soroseris Stebbins by flow cytometry[J]. Plant Science Journal, 2019, 37(6): 775-780. DOI: 10.11913/PSJ.2095-0837.2019.60775

Citation:

PDF (1826 KB)

Estimation of genome size from eight populations of four species of Soroseris Stebbins by flow cytometry

1. School of Life Science, Yunnan Normal University, Kunming 650500, China;
2. Institute of Ecology and Geobotany, School of Ecology and Environment, Yunnan University, Kunming 650500, China

Funds:

This work was supported by grants from the National Natural Science Foundation of China (31670206, 31960046) and Project of Yunnan Natural Science (2018FY001-002).

More Information

Received Date: April 06, 2019
Revised Date: June 16, 2019
Available Online: October 31, 2022
Published Date: December 27, 2019

Graphical Abstract

Abstract

Abstract

In this study, eight populations of four Soroseris Stebbins species were used as experimental materials. The DNA content was determined by flow cytometry based on WPB and Galbraith's buffer and ploidy was determined by traditional chromosome tableting. Results showed that, after comparing the two dissociation buffers, WPB was found to be the most suitable for Soroseris. In addition, S. hirsuta (Anth.) Shih had the smallest genome and the polyploidy level had little effect on the 1Cx-value in the four species. Furthermore, this study also found that seedlings formed by seed germination could be used as experimental materials instead of young leaves that are difficult to obtain.
- Soroseris,
- Flow cytometry,
- Genome size,
- Polyploidy

FullText(HTML)

References (23)

References

[1]	吴征镒. 西藏植物志[M]. 北京:科学出版社, 1987:875-902.
[2]	孙航, 周浙昆. 喜马拉雅东部雅鲁藏布江大峡湾河谷地区植物区系特点及来源[J]. 云南植物研究, 1996, 18(2):852-863. Sun H, Zhou ZK. The characters and origin of the flora from the big bend gorge of Yalutsangpu(Brahmabutra) river, eastern Himalayas[J]. Acta Botanica Yunnanica, 1996, 18(2):852-863.
[3]	Myers N, Mittermeier RA, Mittermeier CG, da Fonseca GA, Kent J. Biodiversity hotspots for conservation priorities[J]. Nature, 2000, 403(6772):853-858.
[4]	Olson DM, Dinerstein E. Priority ecoregions for global conservation[J]. Ann Mo Bot Gard, 2002, 89(2):199-224.
[5]	葛学军, 林有润. 中国植物志:第80卷:第2分册[M]. 北京:科学出版社, 1997:194-202.
[6]	Shi Z, Chen Y, Chen YS, Ling YR, Liu SW, et al. Aste-raceae (Compositae). Flora of China[M]. Beijing:Science Press, 2011.
[7]	Stebbins GL. Studies in the Cichorieae:Dubyaea and Soroseris, endemics of the Sino-Himalayan region[J]. Memoirs of the Torrey Botanical Club, 1940, 19(3):1-76.
[8]	张建文. 菊科绢毛苣属、肉菊属与合头菊属的系统学研究[D]. 昆明:中国科学院昆明植物研究所, 2009.
[9]	Zhang JW, Sun H, Nie ZL. Karyological studies on the Sino-Himalayan endemic Soroseris and two related genera of tribe Lactuceae (Asteraceae)[J]. Bot J Linn Soc, 2007, 154(1):79-87.
[10]	吕建炜. 绢毛苣属植物化学成分及药理作用的研究进展[J]. 辽宁中医药大学学报, 2011(3):212-213. Lu JW. New advances in research of chemical contituents and pharmacological action of genus Soroseris[J]. Journal of Liaoning University of TCM, 2011(3):212-213.
[11]	Bennett MD, Leitch IJ. Nuclear DNA amounts in angiosperms:progress, problems and prospects[J]. Ann Bot, 2005, 95(1):45-90.
[12]	Cavalier-Smith T. The Evolution of Genome Size[M]. Hoboken:John Wiley & Sons, 1985:1-36.
[13]	Bennett MD, Bhandol P, Leitch IJ. Nuclear DNA amounts in angiosperms and their modern uses:807 new estimates[J]. Ann Bot, 2000, 86(4):859-909.
[14]	Zonneveld BJ, Leitch IJ, Bennett MD. First nuclear DNA amounts in more than 300 angiosperms[J]. Ann Bot, 2005, 96(2):229-244.
[15]	李诗琦, 张程, 高信芬. 应用流式细胞术测定17种中国野生蔷薇核DNA含量[J]. 植物科学学报, 2017, 35(4):558-565. Li SQ, Zhang C, Gao XF. Estimation of nuclear DNA content of 17 Chinese wild rose species by flow cytometry[J]. Plant Science Journal, 2017, 35(4):558-565.
[16]	Doležel J, Greilhuber J, Suda J. Estimation of nuclear DNA content in plants using flow cytometry[J]. Nat Protoc, 2007, 2:2233-2244.
[17]	Doležel J, Bartos J. Plant DNA flow cytometry and estimation of nuclear genome size[J]. Ann Bot, 2005, 95:99-110.
[18]	Altinordu F, Peruzzi L, Yan Y, He X. A tool for the analysis of chromosomes:KaryoType[J]. Taxon, 2016, 65(3):586-592.
[19]	International Rice Genome Sequencing Project. The map-based sequence of the rice genome[J]. Nature, 2005, 436(7052):793-800.
[20]	Schnable PS, Ware D, Fulton RS, Stein JC, Wei FS, et al. The B73 Maize genome:complexity, diversity, and dynamics[J]. Science, 2009, 326(5956):1112-1115.
[21]	Givan AL. Flow cytometry:an introduction[J]. Methods Mol Biol, 2011, 699:1-29.
[22]	杨慧娴, 苏富明, 任艳, 孙文光, 张永洪, 李志敏. 青藏高原菊科特有属绢毛菊属核型的补充报道[J]. 植物学研究, 2017, 6(1):17-24. Yang HX, Su FM, Ren Y, Sun WG, Zhang YH, Li ZM. A supplementary of chromosomal studies on an endemic genus Soroseris (Asteraceae:Lactuceae) from the Qinghai-Tibet Plateau[J].Botanical Research, 2017, 6(1):17-24.
[23]	Filip K, Magdalena L, Jakub T,João L, Jan S. Glycerol-treated nuclear suspensions-an efficient preservation method for flow cytometric analysis of plant samples[J]. Chromosome Research, 2012, 20(2):303-315.

[1]	Tian Rui, Wang Liang, Yang Wei, Song Shu-Jie, Niu Yu-Hua, Zou Yang-Jun, Ma Feng-Wang, Li Ming-Jun, Ma Bai-Quan. Effects of exogenous application of humic acid liquid film on photosynthetic characteristics and fruit quality of Malus domestica Borkh.[J]. Plant Science Journal, 2023, 41(5): 668-676. DOI: 10.11913/PSJ.2095-0837.22292
[2]	Shen Su-Yun, Wang Zhou-Qian, Zhang Qi, Yang Jie, Han Fei, Zhong Cai-Hong, Wang Chuan-Hua, Huang Wen-Jun. Analysis of fruit quality and sensory evaluation of 36 kiwifruit (Actinidia) germplasm accessions[J]. Plant Science Journal, 2023, 41(4): 540-551. DOI: 10.11913/PSJ.2095-0837.22300
[3]	Huang Wen-Jun, Ran Xin-Yu, Wang Zhou-Qian, Zhong Cai-Hong. Effects of different storage temperature on fruit quality and storability of Actinidia arguta (Sieb. & Zucc.) Planch. ex Miq. 'Mizao 1'[J]. Plant Science Journal, 2022, 40(5): 695-704. DOI: 10.11913/PSJ.2095-0837.2022.50695
[4]	Han Fei, Zhao Ting-Ting, Liu Xiao-Li, Zhang Qi, Li Da-Wei, Tian Hua, Peng Jue, Zhong Cai-Hong. Genetic analysis of fruit traits in Actinidia rufa (Siebold and Zuccarini) Planchon ex Miquel × Actinidia chinensis var. chinensis C. F. Liang kiwifruit hybrid population[J]. Plant Science Journal, 2022, 40(4): 505-512. DOI: 10.11913/PSJ.2095-0837.2022.40505
[5]	Xiong Hao, Zheng Hao, Han Jia-Xin, Yuan Xin-Yu, Li Ji-Tao, Zhong Cai-Hong, Zhang Qiong. Effects of CPPU treatment on fruit quality in Actinidia[J]. Plant Science Journal, 2022, 40(1): 74-83. DOI: 10.11913/PSJ.2095-0837.2022.10074
[6]	Chen Mei-Yan, Zhao Ting-Ting, Peng Jue, Zhang Peng, Han Fei, Liu Xiao-Li, Zhong Cai-Hong. Multivariate analysis of relationship between soil nutrients and fruit quality in ‘Donghong’ kiwifruit (Actinidia chinensis var. chinensis) orchards[J]. Plant Science Journal, 2021, 39(2): 193-200. DOI: 10.11913/PSJ.2095-0837.2021.20193
[7]	Chen Mei-Yan, Zhao Ting-Ting, Liu Xiao-Li, Han Fei, Zhang Peng, Zhong Cai-Hong. Factor analysis and comprehensive evaluation of fruit quality of ‘Jinyan’ kiwifruit (Actinidia eriantha×Actinidia chinensis)[J]. Plant Science Journal, 2021, 39(1): 85-92. DOI: 10.11913/PSJ.2095-0837.2021.10085
[8]	Chen Mei-Yan, Zhang Peng, Zhao Ting-Ting, Han Fei, Liu Xiao-Li, Zhong Cai-Hong. Relationship between harvest indices and fruit quality traits in Actinidia chinensis ‘Jintao’[J]. Plant Science Journal, 2019, 37(5): 621-627. DOI: 10.11913/PSJ.2095-0837.2019.50621
[9]	Huang Wen-Jun, Liu Xiao-Li, Zhang Qi, Chen Mei-Yan, Zhong Cai-Hong. Research on changes in postharvest physiology and fruit quality of Actinidia chinensis ‘Donghong’ under different storage methods[J]. Plant Science Journal, 2019, 37(3): 382-388. DOI: 10.11913/PSJ.2095-0837.2019.30382
[10]	LI Fang-Min, WANG Jun, CHEN YU-Ping, ZOU Zhi-Rong, WANG Xun-Ling, YUE Ming. The Combined Effects of Enhanced UV-B Radiation and Doubled CO₂ Concentration on the Growth,Fruit Quality and Yield of Tomato in Winter Plastic Greenhouse[J]. Plant Science Journal, 2006, 24(1): 49-53.

Cited By

Get Citation

PDF

XML

Article views PDF downloads

Turn off MathJax

Article Contents

Abstract

References

Estimation of genome size from eight populations of four species of Soroseris Stebbins by flow cytometry

Abstract

References

Related Articles

Catalog

Estimation of genome size from eight populations of four species of Soroseris Stebbins by flow cytometry

Abstract

References

Related Articles

Catalog

Export File

Citation

Format

Content