Study on cell structure, physiology, and biochemistry of vascular bundle blackening in petals of <i>Chimonanthus praecox</i> (L.) Link

Yuan Pu-Ying; Song Xing-Rong; Shao Yi-Fan

doi:10.11913/PSJ.2095-0837.2019.60781

Plant Science Journal > 2019 > 37(6): 781-787. > DOI: 10.11913/PSJ.2095-0837.2019.60781 CSTR: 32231.14.PSJ.2095-0837.2019.60781

Yuan Pu-Ying, Song Xing-Rong, Shao Yi-Fan. Study on cell structure, physiology, and biochemistry of vascular bundle blackening in petals of Chimonanthus praecox (L.) Link[J]. Plant Science Journal, 2019, 37(6): 781-787. DOI: 10.11913/PSJ.2095-0837.2019.60781

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Study on cell structure, physiology, and biochemistry of vascular bundle blackening in petals of Chimonanthus praecox (L.) Link

1. Key Laboratory of Horticultural Crops, Biology and Germplasm Enhancement in Southwest Regions, Ministry of Agriculture, Horticultural Research Institute of Sichuan Academy of Agricultural Sciences, Chengdu 610066, China;
2. College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China

Funds:

This work was supported by grants from the Key Crop Breeding Project in Sichuan Province (2016YZGG-11), Project of Improving Financial Innovation Ability in Sichuan Province (2016ZYPZ-025), Finance Special Fund Project in Sichuan Province (2016CYTS-006), and Project of Improving Financial Innovation Ability in Sichuan Province (2017QNJJ-007).

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Received Date: April 09, 2019
Revised Date: May 06, 2019
Available Online: October 31, 2022
Published Date: December 27, 2019

Graphical Abstract

Abstract

Abstract

We observed the microstructure and ultramicrostructure and measured the physiological and biochemical indices of vascular bundle blackening in petals of Chimonanthus praecox (L.) Link. Results showed that moderately blackened petals exhibited vacuole wall blurring, granular substance increase, and plastid coagulation and blockage. As vascular bundle function was gradually lost, the basic tissue cells became dehydrated, but the epidermal cells remained relatively full to maintain basic flower functions. As the degree of vascular bundle blackening intensified, peroxidase (POD) activity and free amino acid content increased, whereas the soluble sugar content decreased, consistent with the physiological characteristics of flower senescence. However, malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, and soluble protein content did not decrease significantly, indicating that the C. praecox flower possessed a strong ability to adapt to adversity, and the degree of membrane lipid peroxidation was not high. In addition, the membrane and internal structures of most cells were relatively undamaged. This study provides a theoretical basis for further study on the formation mechanism of this phenomenon and the final solution to this problem.
- Chimonanthus praecox,
- Vascular bundle blackening,
- Cell structure,
- Physiology and biochemistry

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References (33)

References

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