Variation in genome size and stomatal traits among three <i>Sorbus</i> species

Xi Lian-Lian; Li Jia-Bao; Zhu Kai-Lin; Qi Qi; Chen Xin

doi:10.11913/PSJ.2095-0837.2020.10032

Plant Science Journal > 2020 > 38(1): 32-38. > DOI: 10.11913/PSJ.2095-0837.2020.10032 CSTR: 32231.14.PSJ.2095-0837.2020.10032

Xi Lian-Lian, Li Jia-Bao, Zhu Kai-Lin, Qi Qi, Chen Xin. Variation in genome size and stomatal traits among three Sorbus species[J]. Plant Science Journal, 2020, 38(1): 32-38. DOI: 10.11913/PSJ.2095-0837.2020.10032

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Variation in genome size and stomatal traits among three Sorbus species

Xi Lian-Lian^1,2,
Li Jia-Bao^1,2,
Zhu Kai-Lin^1,2,
Qi Qi^1,2,
Chen Xin^1,2

1. Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China;
2. College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China

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This work was supported by a grant from the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Received Date: June 22, 2019
Revised Date: September 18, 2019
Available Online: October 31, 2022
Published Date: February 27, 2020

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Abstract

Abstract

In this paper, ploidy in Sorbus cibagouensis H. Peng & Z. J. Yin, S. hypoglauca (Cardot) Hand.-Mazz., and S. vilmorinii C. K. Schneid was assessed by means of relative genome size using flow cytometry. At the same time, stomatal characteristics were observed by light microscopy and scanning electron microscopy. Based on flow cytometry profiles, the genome sizes of S. cibagouensis, S. hypoglauca, and S. vilmorinii were (1.480±0.039) pg, (1.513±0.041) pg, and (2.675±0.065) pg respectively. The cytotypes of S. cibagouensis (2x), S. hypoglauca (2x), and S. vilmorinii (4x) were also revealed. The stomata of the three Sorbus species did not sink and the guard cells had no ‘T’ type thickening structure. Furthermore, the stomata were distributed in the lower epidermis of the leaves and were anomocytic type. The outer stomatal rim ornamentation of S. cibagouensis and S. vilmorinii was smooth, whereas that of S. hypoglauca was short rod waxy. These results showed that there were significant differences in stomatal size among the three Sorbus species. Furthermore, genome size was positively associated with the ploidy of Sorbus, which could therefore be used for ploidy identification. Although the correlation between stomatal traits and ploidy was not significant, the stomatal characteristics changed significantly among species, which could provide a theoretical basis for species identification.
- Genome size,
- Ploidy,
- Stomatal traits,
- Sorbus

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References (35)

References

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