Establishment of flow cytometry system for DNA C-value determination of polyploid species in subgenus <i>Yulania</i>

Zou Xuan; Sun Li-Yong; Wan Xiao-Xia; Chen Yao; Lin Feng; Yin Zeng-Fang

doi:10.11913/PSJ.2095-0837.2020.30369

Plant Science Journal > 2020 > 38(3): 369-377. > DOI: 10.11913/PSJ.2095-0837.2020.30369 CSTR: 32231.14.PSJ.2095-0837.2020.30369

Zou Xuan, Sun Li-Yong, Wan Xiao-Xia, Chen Yao, Lin Feng, Yin Zeng-Fang. Establishment of flow cytometry system for DNA C-value determination of polyploid species in subgenus Yulania[J]. Plant Science Journal, 2020, 38(3): 369-377. DOI: 10.11913/PSJ.2095-0837.2020.30369

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Establishment of flow cytometry system for DNA C-value determination of polyploid species in subgenus Yulania

1. Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China;
2. Advanced Analysis and Testing Center, Nanjing Forestry University, Nanjing 210037, China

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This work was supported by grants from the Natural and Science Foundation of Jiangsu Province (BK20171451) and Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Received Date: September 24, 2019
Revised Date: November 13, 2019
Available Online: October 31, 2022
Published Date: June 27, 2020

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Abstract

Abstract

By screening different combinations of dissociation solutions and internal standards, the DNA C-values of six polyploid plants of subgen. Yulania (Magnoliaceae) were determined and analyzed by flow cytometry. Results showed that, in the screening of solution buffers and standard samples, woody plant buffer (WPB) was the best choice for subgen. Yulania. Furthermore, the peak pattern of mixed samples worked best when Magnolia stellata (Siebold & Zucc.) Maxim. was the internal standard and the coefficient of variation (CV) of the DNA peak was less than 5.0%. The C-values of M. liliiflora Desr., M. polytepala Law, R. Z. Zhou & R. J. Zhang, M. cylindrica E. H. Wilson, M. denudata Desr., M.×soulangeana Soul.-Bod., and M. acuminata (L.) L ranged from (3.92 ±0.02) pg to (5.85 ±0.03) pg and the genome size ranged from (3832.13 ±24.67) Mbp to (5964.17 ±56.06) Mbp. Thus, our results showed that the optimized flow cytometry system accurately determined the DNA C-value of polyploid species in the subgen. Yulania.
- Flow cytometry,
- DNA C-value,
- Genome size,
- subgenus Yulania

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References (31)

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Establishment of flow cytometry system for DNA C-value determination of polyploid species in subgenus Yulania

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