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Dai Huan-Huan, Shan Yu-Si, Xin Zheng-Qi, He Xiao, Wu Neng-Biao. Effects of exogenous betaine on physiological characteristics and tropidine alkaloid content in Atropa belladonna L. under NaCl stress[J]. Plant Science Journal, 2020, 38(3): 400-409. DOI: 10.11913/PSJ.2095-0837.2020.30400
Citation: Dai Huan-Huan, Shan Yu-Si, Xin Zheng-Qi, He Xiao, Wu Neng-Biao. Effects of exogenous betaine on physiological characteristics and tropidine alkaloid content in Atropa belladonna L. under NaCl stress[J]. Plant Science Journal, 2020, 38(3): 400-409. DOI: 10.11913/PSJ.2095-0837.2020.30400
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Effects of exogenous betaine on physiological characteristics and tropidine alkaloid content in Atropa belladonna L. under NaCl stress

  • Key Laboratory of Eco-Environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Science, Southwest University, Chongqing 400715, China

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This work was supported by grants from the National Natural Science Foundation of China (30500041), and Chongqing Science and Technology Research Project (cstc2012gg-yyjs80013).

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  • Received Date: August 07, 2019
  • Revised Date: October 08, 2019
  • Available Online: October 31, 2022
  • Published Date: June 27, 2020
    Graphical Abstract
    • Abstract
  • In this study, Atropa belladonna L. seedlings were sprayed with different concentrations (20 mmol/L and 40 mmol/L) of betaine (glycine betaine, GB) under artificial simulated 100 mmol/L salt stress to investigate the effects on physiological characteristics and tropane alkaloid content in Atropa belladonna L. Compared with NaCl stress treatment, exogenous betaine enhanced the photosynthesis of Atropa belladonna L. leaves, with superoxide (SOD), peroxidase (POD), and catalase (CAT) activities increasing to different degrees; malondialdehyde (MDA) content decreased significantly; nitrate nitrogen, soluble protein, and free amino acid content and nitrate reductase (NR), glutamine synthetase (GS), and glutamate dehydrogenase (GDH) activities increased significantly. The contents of precursor amino acids (ornithine and arginine) and tropane alkaloids (scopolamine and scopolamine) in the secondary metabolic pathway of Atropa belladonna L. also significantly increased. Our results indicated that 100 mmol/L salt stress treatment inhibited the growth of belladonna, and 20 mmol/L betaine treatment could alleviate this damage.
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    • References (35)
  • [1]
    卢克欢, 刘兴, 杨怡, 廖志华, 吴能表. UV-B胁迫下Ca2+对颠茄生理特性与次生代谢产物的调控研究[J]. 作物学报, 2018, 44(10):1527-1538.

    Lu KH, Liu X, Yang Y, Liao ZH, Wu NB. Effect of exogenous Ca2+ on physiological characteristics and secondary metabolites accumulation of Atropa belladonna L. seedlings under UV-B stress[J]. Acta Agronomica Sinica, 2018, 44(10):1527-1538.
    [2]
    寇江涛, 康文娟, 苗阳阳, 师尚礼.外源2,4-表油菜素内酯对NaCl胁迫下紫花苜蓿幼苗光合特性及离子吸收、运输和分配的影响[J]. 草业学报, 2016, 25(4):91-103.

    Kou JT, Kang WJ, Miao YY, Shi SL. Effects of exogenous2,4-epibrassinolide on the uptake, transport, and disputation of ions, and photosynthetic characteristics of Medicago sativa seedlings under NaCl stress[J]. Acta Prata-culturae Sinica, 2016, 25(4):91-103.
    [3]
    张天鹏, 杨兴洪. 甜菜碱提高植物抗逆性及促进生长发育研究进展[J]. 植物生理学报, 2017, 53(11):1955-1962.

    Zhang TP, Yang XH. Research on the mechanism of glycinebetaine regulating plants stress resistance and development[J]. Plant Physiology Journal, 2017, 53(11):1955-1962.
    [4]
    杨洪兵. 甜菜碱对盐胁迫下荞麦幼苗生理特性的影响[J]. 贵州农业科学, 2013, 41(8):51-53.

    Yang HB. Effect of betaine on physiological characteristics of buckwheat seedlings under salt stress[J]. Guizhou Agricultural Sciences, 2013, 41(8):51-53.
    [5]
    Singh M, Kumar J, Singh VP. Roles of osmoprotectants in improving salinity and drought tolerance in plants:a review[J]. Rev Environ Sci Bio, 2015, 14(3):407-426.
    [6]
    李小玲, 华智锐, 郭青青. 甜菜碱对盐胁迫黄芩抗逆性的影响[J]. 贵州农业科学, 2018, 46(1):77-81.

    Li XL, Hua ZR, Guo QQ. Effect of betaine on stress resis-tance of Scutellaria baicalensis under salt stress[J]. Guizhou Agricultural Sciences, 2018, 46(1):77-81.
    [7]
    严青青, 张巨松, 代健敏, 窦巧巧. 甜菜碱对盐碱胁迫下海岛棉幼苗光合作用及生物量积累的影响[J]. 作物学报, 2019, 12(5):1-8.

    Yan QQ, Zhang JS, Dai JM, Dou QQ. Effects of glycinebetain on photosynthesis and biomass accumulation of island cotton seedlings under saline alkali stress[J]. Acta Agronomica Sinica, 2019, 12(5):1-8.
    [8]
    张红敏, 李姣姣, 黑刚刚, 曹瑞霞, 周心渝, 李琳琳, 等. 外源甜菜碱处理对干旱胁迫下半夏氮代谢及相关酶活性的影响[J]. 草业学报, 2014, 23(4):229-236.

    Zhang HM, Li JJ, Hei GG, Cao RX, Zhou XY, Li LL, et al. Effects of exogenous betaine on the secondary metabolites of Pinellia ternata under drought stress[J]. Acta Prataculturae Sinica, 2014, 23(4):229-236.
    [9]
    卢克欢, 郭双, 韦悦, 张翠平, 吴能表. 外源性5-氨基乙酰丙酸对盐胁迫下颠茄生理特性及次生代谢产物含量的影响[J]. 西南大学学报(自然科学版), 2018, 40(9):13-20.

    Lu KH, Guo S, Wei Y, Zhang CP, Wu NB. Effects of exogenous 5-aminolevulinic acid on physiological characte-ristics and secondary metabolite contents of Atropa belladonna L. seedlings under NaCl stress[J]. Journal of Southwest University (Natural Science Edition), 2018, 40(9):13-20.
    [10]
    张宪政. 植物叶绿素含量测定:丙酮乙醇混合液法[J]. 辽宁农业科学, 1986(3):26-28
    [11]
    吴雪霞, 于力, 朱为民. 外源一氧化氮对NaCl胁迫下番茄幼苗叶绿素荧光特性的影响[J]. 中国生态农业学报, 2009, 17(4):746-751.

    Wu XX, Yu L, Zhu WM. Effect of exogenous nitric oxide on chlorophyll fluorescence characteristics in tomato seedlings under NaCl stress[J]. Chinese Journal of Eco-Agriculture, 2009, 17(4):746-751.
    [12]
    高俊凤, 植物生物学试验指导[M]. 北京:高等教育出版社, 2006:208-230.
    [13]
    Velikova V, Yordanov I, Edreva A. Oxidative stress and some antioxidant systems in acid rain 2-treated bean plants protective role of exogenous polyamines[J]. Plant Science, 2000, 151(2):59-66.
    [14]
    张志良, 瞿伟菁, 李小方. 植物生理学实验指导[M]. 北京:高等教育出版社, 2009:103-126.
    [15]
    Giannopolitis CN, Ries SK. Superoxide dismutases:Ⅰ. Occurrence in higher plants[J]. Plant Physiol, 1977, 59(2):309-314.
    [16]
    Omran RG. Peroxide levels and the activities of catalase, peroxidase, and indoleacetic acid oxidase during and after chilling cucumber seedlings[J]. Plant Physiol, 1980, 65(2):407-408.
    [17]
    李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社, 2000:192-194.
    [18]
    王小纯, 熊淑萍, 马新明, 张娟娟, 王志强. 不同形态氮素对专用型小麦花后氮代谢关键酶活性及籽粒蛋白质含量的影响[J]. 生态学报, 2005, 25(4):802-807.

    Wang XC, Xiong SP, Ma XM, Zhang JJ, Wang ZQ. Effects of different nitrogen forms on key enzyme activity involved in nitrogen metabolism and grain protein content in speciality wheat cultivars[J]. Acta Ecologica Sinica, 2005, 25(4):802-807.
    [19]
    黄爱清, 苏国成, 王璋. L-鸟氨酸快速定量检测方法[J].食品与发酵工业, 2005, 31(12):98-102.

    Huang AQ, Su GC, Wang Z. Rapid quantitative determination for L-ornithine analysis[J]. Food and Fermentation Industries, 2005, 31(12):98-102.
    [20]
    胡桂娟, 刘寄明, 刘嘉芬. 化学法测定精氨酸总量[J]. 落叶果树, 1995(1):22.
    [21]
    Zárate R, Hermosin B, Cantos M, et al. Tropane alkaloid distribution in Atropa baetica plants[J]. J Chem Ecol, 1997, 23(8):2059-2066.
    [22]
    张守仁. 叶绿素荧光动力学参数的意义及讨论[J]. 植物学通报, 1999, 16(4):444-448.

    Zhang SR. A discussion on chlorophyll fluorescence kine-tics parameters and their significance[J]. Chinese Bulletin of Botang, 1999, 16(4):444-448.
    [23]
    郝敬虹, 易旸, 尚庆茂, 董春娟, 张志刚. 水杨酸处理对干旱胁迫下黄瓜幼苗氮素同化及其关键酶活性的影响[J]. 园艺学报, 2012, 39(1):81-90.

    Hao JH, Yi Y, Shang QM, Dong CJ, Zhang ZG. Effect of exogenous salicylic acid on nitrogen assimilation of cucumber seedling under drought stress[J]. Acta Horticulturae Sinica, 2012, 39(1):81-90.
    [24]
    米永伟, 陈垣, 郭凤霞, 王龙强. 盐胁迫下黑果枸杞幼苗对外源甜菜碱的生理响应[J].草业科学, 2012, 29(9):1417-1421.

    Mi YW, Chen Y, Guo FX, Wang LQ. Physiological response of Lycium ruthenicum seedling to exogenous be-taine under salt stress[J]. Pratacultural Science, 2012, 29(9):1417-1421.
    [25]
    Lu CM, Zhang JH. Effects of water stress on photosystemⅡ photochemistry and its thermostability in wheat plants[J]. J Exp Bot, 1999, 50(336):1199-1206.
    [26]
    Mittler R. Oxidative stress, antioxidants and stress tole-rance[J]. Trends Plant Sci, 2002, 7(9):405-410.
    [27]
    李文兵, 王燕凌, 李芳, 刘君, 李霞. 水分胁迫下多枝柽柳体内活性氧与保护酶的关系[J]. 新疆农业大学学报, 2007, 30(1):30-34.

    Li WB, Wang YL, Li F, Liu J, Li X. Relationship between active oxygen and protective enzymes in Tamarix ramosissima under water stress[J]. Journal of Xinjiang Agricultu-ral University, 2007, 30(1):30-34.
    [28]
    董文科, 马祥, 周学文, 路旭平, 王佳, 何娟, 等. 外源甜菜碱对低温胁迫下紫花苜蓿幼苗生理特性的影响[J]. 草地学报, 2019, 27(1):130-140.

    Dong WK, Ma X, Zhou XW, Lu XP, Wang J, He J, et al. Effects of exogenous glycine betaine on the physiological characteristics in Medicago sative seedlings under low-temperature stress[J]. Acta Agrestia Sinica, 2019, 27(1):130-140.
    [29]
    范春丽, 曲金柱. 甜菜碱浸种对盐胁迫下石榴种子萌发及幼苗生理的影响[J]. 中国南方果树, 2016, 45(1):86-90.
    [30]
    吴雪霞, 查丁石, 朱宗文, 李贤. 外源24-表油菜素内酯对盐胁迫下茄子种子萌发和幼苗生理特性的影响[J]. 植物生理学报, 2011, 47(6):607-612.

    Wu XX, Zha DS, Zhu ZW, Li X. Effects of exogenous 24-epibrassinolide on seed germination, physiological characteristics of eggplant seedlings under NaCl stress[J]. Plant Physiology Journal, 2011, 47(6):607-612.
    [31]
    赵金莉, 牛贵月. 外源甜菜碱对黄瓜抗盐胁迫的影响[J]. 河南农业科学, 2009(6):112-115.

    Zhao JL, Niu GY. Effects of exogenous glycinebetaine on salt resistance of cucumber[J]. Journal of Henan Agricultural Sciences, 2009(6):112-115.
    [32]
    郭双. 酵母提取物对颠茄毛状根碳氮代谢及托品烷类生物碱代谢的调控研究[D]. 西南大学, 2018.
    [33]
    程梦云, 苟剑喻, 杨茂, 赵建, 彭玉龙, 黄莺. 不同镁浓度对烟叶氮代谢关键酶活性的影响[J]. 山地农业生物学报, 2019, 38(2):48-51, 70.

    Cheng MY, Gou JY, Yang M, Zhao J, Peng YL, Huang Y. Effects of magnesium concentration on the activities of key enzymes of nitrogen metabolism in tobacco leaves[J]. Journal of Mountain Agriculture and Biology, 2019, 38(2):48-51, 70.
    [34]
    扈雪欢, 宁欢欢, 刘光照, 吴能表. 外源SA对盐胁迫下颠茄生理生化、氮代谢及次生代谢的影响[J]. 草业学报, 2017, 26(11):147-156.

    Hu XH, Ning HH, Liu GZ, Wu NB. Effect of exogenous SA on the physiological biochemistry, nitrogen metabolism and secondary metabolism of Atropa belladonna under NaCl stress[J]. Acta Prataculturae Sinica, 2017, 26(11):147-156.
    [35]
    张毅, 石玉, 胡晓辉, 邹志荣, 曹凯, 张浩. 外源Spd对盐碱胁迫下番茄幼苗氮代谢及主要矿质元素含量的影响[J]. 应用生态学报, 2013, 24(5):1401-1408.

    Zhang Y, Shi Y, Hu XH, Zou ZR, Cao K, Zhang H. Effects of exogenous spermidine on the nitrogen metabolism and main mineral elements contents of tomato seedlings under saline-alkali stress[J]. Chinese Journal of Applied Ecology, 2013, 24(5):1401-1408.
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