Transcriptome sequencing and expression analysis of <i>Batrachium bungei</i> (Steud.) L. Liou at different altitudes based on RNA-seq in the Qinghai-Tibet Plateau

Chen Fei-Fei; Ji Ya-Li; Chen Zhong-Hai; Liu Tai-Long; Liu Xing

doi:10.11913/PSJ.2095-0837.2021.10050

Plant Science Journal > 2021 > 39(1): 50-58. > DOI: 10.11913/PSJ.2095-0837.2021.10050 CSTR: 32231.14.PSJ.2095-0837.2021.10050

Chen Fei-Fei, Ji Ya-Li, Chen Zhong-Hai, Liu Tai-Long, Liu Xing. Transcriptome sequencing and expression analysis of Batrachium bungei (Steud.) L. Liou at different altitudes based on RNA-seq in the Qinghai-Tibet Plateau[J]. Plant Science Journal, 2021, 39(1): 50-58. DOI: 10.11913/PSJ.2095-0837.2021.10050

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Transcriptome sequencing and expression analysis of Batrachium bungei (Steud.) L. Liou at different altitudes based on RNA-seq in the Qinghai-Tibet Plateau

Chen Fei-Fei^1,2,
Ji Ya-Li^1,2,
Chen Zhong-Hai^1,2,
Liu Tai-Long^1,2,
Liu Xing^1,2,3, ,

1. College of Science, Tibet University, Lhasa 850000, China;
2. Research Center for Ecology and Environment of Qinghai-Tibetan Plateau, Tibet University, Extreme Environmental Biological Adaptation and Evolution Laboratory, Lhasa 850000, China;
3. College of Life Sciences, Wuhan University, Wuhan 430072, China

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This work was supported by a grant from the National Natural Science Foundation of China (31860046).

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Received Date: June 17, 2020
Revised Date: September 29, 2020
Available Online: October 31, 2022
Published Date: February 27, 2021

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Abstract

Abstract

We explored how Batrachium bungei (Steud.) L. Liou has adapted to extreme high-altitude environments. We used transcriptome sequencing and bioinformatics analysis of seven populations of B. bungei (ML, MZ, WQ, YH, YBJ, XB, ZR). Based on Pearson correlation analysis and principal component analysis (PCA), results showed that the gene expression of each population was highly similar. Differential gene enrichment analysis showed that phenylpropanoid biosynthesis was significantly enriched in five different groups. In addition, flavonoid biosynthesis, carotenoid biosynthesis, phenylalanine tyrosine and tryptophan biosynthesis, plant hormone signaling transduction, MAPK signaling pathway, and plant-pathogen interaction were significantly enriched. Compared with low-altitude populations, flavonoid biosynthesis genes (HHT1, HCT, F3'H, CHS, CYP73A, CCOAOMT5, CYP98A) were significantly up-regulated in the high-altitude populations, and their expression levels were significantly higher than those in the low-altitude (MZ) population. These results indicate that B. bungei adapted to the high-altitude environment of the Qinghai-Tibet Plateau mainly through multi-pathway participation and the regulation and expression of key genes.
- Batrachium bungei,
- Transcriptome sequencing,
- Differential expression,
- Enrichment analysis,
- Flavonoids

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References (34)

References

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