Advance Search
Li Ying, Zhang Yue-Jing, Wang Xin, Pang Hai-Long, Jia Ling-Yun, Feng Han-Qing. Effects of Agrobacterium tumefaciens strain and its infection time and concentration on transient expression of foreign genes based on expression vector of bean yellow dwarf virus[J]. Plant Science Journal, 2021, 39(3): 297-305. DOI: 10.11913/PSJ.2095-0837.2021.30297
Citation: Li Ying, Zhang Yue-Jing, Wang Xin, Pang Hai-Long, Jia Ling-Yun, Feng Han-Qing. Effects of Agrobacterium tumefaciens strain and its infection time and concentration on transient expression of foreign genes based on expression vector of bean yellow dwarf virus[J]. Plant Science Journal, 2021, 39(3): 297-305. DOI: 10.11913/PSJ.2095-0837.2021.30297
shu

Effects of Agrobacterium tumefaciens strain and its infection time and concentration on transient expression of foreign genes based on expression vector of bean yellow dwarf virus

  • College of Life Sciences, Northwest Normal University, Lanzhou 730070, China

Funds: 

This work was supported by grants from the National Natural Science Foundation of China (31870246，31560070), University Scientific Research Project of Gansu Province(2015A-007), Key Research and Development Project of Gansu Province (18YF1NA051), Fundamental Research Funds for the Gansu Universities of Gansu Provincial Department of Finance, and Youth Innovation Team of Northwest Normal University.

More Information
  • Received Date: October 29, 2020
  • Revised Date: November 29, 2020
  • Available Online: October 31, 2022
  • Published Date: June 27, 2021
    Graphical Abstract
    • Abstract
  • To explore the effects of different Agrobacterium tumefaciens strains and their time and concentration on the transient expression of foreign genes based on geminivirus expression vectors, three common A. tumefaciens strains and replicative plant expression vectors based on the bean yellow dwarf virus (BeYDV) were used as experimental materials. [JP+1]The exogenous green fluorescent protein (GFP) gene was introduced into Nicotiana benthamiana L. leaves to achieve transient expression by transient transformation mediated by A. tumefaciens. The effects of different A. tumefaciens strains, concentration, and time on transient expression were compared. Results showed significant differences among the three different strains of A. tumefaciens in the transient expression of GFP in N. benthamiana leaves. Among them, the transformation efficiency of strain EHA105 was the highest, followed by LBA4404, and then GV3101. In addition, the optimum concentrations of GV3101, EHA105, and LBA4404 were 0.5, 0.3, and 0.3, respectively, and the best time was 4 d. These results suggest that the differences in chromosome structure and Ti plasmid of the A. tumefaciens strains are important factors affecting the concentration of A. tumefaciens and the instantaneous expression efficiency of foreign genes during transient transformation.
    • FullText(HTML)
    • References (32)
  • [1]
    赵文婷, 魏建和, 刘晓东, 高志晖. 植物瞬时表达技术的主要方法与应用进展[J]. 生物技术通讯, 2013, 24(2):294-300.

    Zhao WT, Wei JH, Liu XD, Gao ZH. Advance of the main methods and applications of plant transient expression system[J]. Letters in Biotechnology, 2013, 24(2):294-300.
    [2]
    Chen Q, Lai H, Hurtado J, Stahnke J, Leuzinger K, Dent M. Agroinfiltration as an effective and scalable strategy of gene delivery for production of pharmaceutical proteins[J]. Adv Tech Biol Med, 2013, 1(1):103.
    [3]
    Leuzinger K, Dent M, Hurtado J, Stahnke J, Lai H, et al. Efficient agroinfiltration of plants for high-level transient expression of recombinant proteins[J]. J Vis Exp, 2013, (77):50521.
    [4]
    Peyret H, Lomonossoff GP. When plant virology met Agrobacterium:the rise of the deconstructed clones[J]. Plant Biotechnol J, 2015, 13(8):1121-1135.
    [5]
    Abrahamian P, Hammond RW, Hammond J. Plant virus-derived vectors:applications in agricultural and medical biotechnology[J]. Annu Rev Virol, 2020, 7(1):513-535.
    [6]
    Hefferon KL. DNA virus vectors for vaccine production in plants:spotlight on geminiviruses[J]. Vaccines (Basel), 2014, 2(3):642-653.
    [7]
    Rybicki EP, Martin DP. Virus-derived ssDNA vectors for the expression of foreign proteins in plants[J]. Curr Top Microbiol Immunol, 2014, 375:19-45.
    [8]
    Chen Q, He J, Phoolcharoen W, Mason HS. Geminiviral vectors based on bean yellow dwarf virus for production of vaccine antigens and monoclonal antibodies in plants[J]. Hum Vaccin, 2011, 7(3):331-338.
    [9]
    Zaidi SS, Mansoor S. Viral vectors for plant genome engineering[J]. Front Plant Sci, 2017, 8:539.
    [10]
    Zhang X, Mason H. Bean yellow dwarf virus replicons for high-level transgene expression in transgenic plants and cell cultures[J]. Biotechnol Bioeng, 2006, 93(2):271-279.
    [11]
    Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF. DNA replicons for plant genome engineering[J]. Plant Cell, 2014, 26(1):151-163.
    [12]
    Richter KS, Serra H, White CI, Jeske H. The recombination mediator RAD51D promotes geminiviral infection[J]. Virology, 2016, 493:113-127.
    [13]
    Hanley-Bowdoin L, Bejarano ER, Robertson D, Mansoor S. Geminiviruses:masters at redirecting and reprogramming plant processes[J]. Nat Rev Microbiol, 2013, 11(11):777-788.
    [14]
    邱礽, 陶刚, 李奇科, 邱又彬, 刘作易. 农杆菌渗入法介导的基因瞬时表达技术及应用[J]. 分子植物育种, 2009, 7(5):1032-1039.

    Qiu R, Tao G, Li QK, Qiu YB, Liu ZY. Transient gene expression mediated by agroinfiltration and its application[J]. Molecular Plant Breeding, 2009, 7(5):1032-1039.
    [15]
    叶文兴, 孔令琪. 影响根癌农杆菌转化效率的因素综述[J]. 中国草地学报, 2019, 41(3):142-148.

    Ye WX, Kong LQ. Advanced in factors influencing agrobacterium tumefaciens-mediates transformation efficiency[J]. Chinese Journal of Grassland, 2019, 41(3):142-148.
    [16]
    Hellens R, Mullineaux P, Klee H. Technical focus:a guide to Agrobacterium binary Ti vectors[J]. Trends Plant Sci, 2000, 5(10):446-451.
    [17]
    王从丽, 陆柏方, 张学成, 李广旭, 王劲波, 等. 农杆菌——植物间基因转移的分子基础[J]. 生命科学, 2002(1):1-5.

    Wang CL, Lu BF, Zhang XC, Li GX, Wang JB, et al. Molecular basis of Agrobacterium-plant gene transfer[J]. Chinese Bulletin of Life Sciences, 2002(1):1-5.
    [18]
    李东栋, 石玮, 邓秀新, 伊华林. 不同根癌农杆菌菌株对柑橘愈伤组织遗传转化效率的影响[J]. 华中农业大学学报, 2002(4):379-381.

    Li DD, Shi W, Deng XX, Yi HL. Influence of different strains on Agrobacterium-mediated callus transformation efficiency in citrus[J]. Journal of Huazhong Agricultural University, 2002(4):379-381.
    [19]
    Gelvin SB. Agrobacterium-mediated plant transformation:the biology behind the "gene-jockeying" tool[J]. Micro-biol Mol Biol Rev, 2003, 67(1):16-37.
    [20]
    邹智, 卢长明. 影响农杆菌介导遗传转化的植物因子研究进展[J]. 生物技术通报, 2008(1):1-9.

    Zou Z, Lu CM. An Overview of plant factors influencing Agrobacterium-mediated transformation[J]. Biotechnology Bulletin, 2008(1):1-9.
    [21]
    李卫, 董文, 周菲, 郭光沁, 郑国錩. 参与在农杆菌介导遗传转化过程中的植物因子研究进展[J]. 中国生物工程杂志, 2003(12):62-67.

    Li W, Dong W, Zhou F, Guo GQ, Zhen GC. Progress of study on plant factors that participated in Agrobacterium tumefaciens-mediated transformation[J]. China Biotechnology, 2003(12):62-67.
    [22]
    郭晓丽. 根癌农杆菌介导植物遗传转化的分子机制[J]. 衡水学院学报, 2008(1):52-54.

    Guo XL. Molecular basis of Agrobacterium tumefaciens-mediated transformation for plant[J]. Journal of Hengshui University, 2008(1):52-54.
    [23]
    He F, Nair GR, Soto CS, Chang Y, Hsu L, et al. Molecular basis of ChvE function in sugar binding, sugar utilization, and virulence in Agrobacterium tumefaciens[J]. J Bacteriol, 2009, 191(18):5802-5813.
    [24]
    王根平, 张婷, 师志刚, 程汝宏. 农杆菌T-DNA传递相关基因研究进展[J]. 分子植物育种, 2017, 15(5):1752-1761.

    Wang GP, Zhang T, Shi ZG, Cheng RH. Research progress on T-DNA transfer related genes mediated of Agrobacterium[J]. Molecular Plant Breeding, 2017, 15(5):1752-1761.
    [25]
    Gelvin SB. Agrobacterium and plant genes involved in T-DNA transfer and integration[J]. Annu Rev Plant Physiol Plant Mol Biol, 2000, 51:223-256.
    [26]
    Sun Y, Qian H, Xu XD, Han Y, Yen LF, Sun DY. Integrin-like proteins in the pollen tube:detection, localization and function[J]. Plant Cell Physiol, 2000, 41(10):1136-1142.
    [27]
    Ditt RF, Nester EU, Comai L. Plant gene expression response to Agrobacterium tumefaciens[J]. Proc Natl Acad Sci USA, 2001, 98(19):10954-10959.
    [28]
    Poborilova Z, Plchova H, Cerovska N, Gunter CJ, Hitzeroth II, et al. Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors[J]. Plant Cell Rep, 2020, 39(9):1115-1127.
    [29]
    James E, Lee JM. The production of foreign proteins from genetically modified plant cells[J]. Adv Biochem Eng Biotechnol, 2001, 72:127-156.
    [30]
    Holland T, Sack M, Rademacher T, Schmale K, Altmann F, et al. Optimal nitrogen supply as a key to increased and sustained production of a monoclonal full-size antibody in BY-2 suspension culture[J]. Biotechnol Bioeng, 2010, 107(2):278-289.
    [31]
    Tsoi BM, Doran PM. Effect of medium properties and additives on antibody stability and accumulation in suspended plant cell cultures[J]. Biotechnol Appl Biochem, 2002, 35(3):171-180.
    [32]
    Raven N, Schillberg S, Rasche S. Plant cell-based recombinant antibody manufacturing with a 200 L orbitally shaken disposable bioreactor[J]. Methods Mol Biol, 2016, 1385:161-172.
    • Related Articles

  • Related Articles

    [1]Liu Fenfen, Mo Liangtuan, Ou Guoteng, Nie Yimei, Niu Tao, Huang Qinjun. Genetic diversity and genetic structure analysis of wild Chinese Rosa roxburghii Tratt. germplasm resources[J]. Plant Science Journal, 2024, 42(3): 350-358. DOI: 10.11913/PSJ.2095-0837.23248
    [2]ZHAO Jie, WANG Bin-Qi, JIA Xiao, TONG Yi-Qin, HE Yi-Fa, GE Tai-Ming. Development of SSR Markers to Assess Genetic Diversity in Osmunda japonica Thunb.[J]. Plant Science Journal, 2015, 33(6): 801-807. DOI: 10.11913/PSJ.2095-0837.2015.60801
    [3]TAO Ai-Fen, QI Jian-Min, SU Jian-Guang, FANG Ping-Ping, LIN Li-Hui, XU Jian-Tang, WU Jian-Mei, LIN Pei-Qing. Analysis of Genetic Diversity of Jute (Corchorus L.) Germplasm Revealed by SRAP[J]. Plant Science Journal, 2012, (2): 178-187. DOI: 10.3724/SP.J.1142.2012.20178
    [4]MA Rui-Jun, LU Jian-Ying. Genetic Diversity of Ground Cover Plant Potentilla anserina L.[J]. Plant Science Journal, 2010, 28(4): 473-479.
    [5]QIN Yong-Yan, WANG Yi-Ling, ZHANG Qin-Di, BI Run-Cheng, YAN Gui-Qin. Analysis on the Population Genetic Diversity of an Endangered Plant (Elaeagnus mollis) by SSR Markers[J]. Plant Science Journal, 2010, 28(4): 466-472.
    [6]ZHOU Yuan, GAO Lei, WANG Zhi-Wei, WANG Ting. Application of Molecular Marker Techniques in Genetic Diversity of Pteridophytes[J]. Plant Science Journal, 2009, 27(6): 667-673.
    [7]REN Xiao-Ping, JIANG Hui-Fang, LIAO Bo-Shou, LEI Yong, WANG Sheng-Yu, LI Dong. Genetic Diversity of Arachis hypogaea var.hirsute in Peanut[J]. Plant Science Journal, 2007, 25(4): 401-405.
    [8]XIA Jing, GUO You-Hao. ISSR Analysis for Genetic Diversity of Pedicularis dunniana[J]. Plant Science Journal, 2006, 24(6): 565-568.
    [9]BIN Xiao-Yun, TANG Shao-Qing, ZHOU Jun-Ya, SONG Hong-Tao, LI Zun-Yi. ISSR Analysis on Genetic Diversity of Camellia nitidissima Chi (Theaceae) in China[J]. Plant Science Journal, 2005, 23(1): 20-26.
    [10]CHENG Zhong-Ping, CHEN Zhi-Wei, HU Chun-Gen, DENG Xiu-Xin. Study on Genetic Diversity of Amygdalus persica Based on RAPD Markers[J]. Plant Science Journal, 2002, 20(2): 89-99.

Catalog

    Get Citation
    PDF
    XML
    Article views (948) PDF downloads (518) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles
    Copyright © 2022 Plant Science Journal 鄂ICP备05004779号-3
    Address：No. 201, Jiufeng 1st Road, Donghu High tech Zone, WuhanPostal Code：430074

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return