Physiological responses of seed dormancy and germination to cold stratification in <i>Phoebe hui</i> Cheng ex Yang

Zhang Xin-Yi; Yan Xu; Li Tie-Hua; Li Yang-Ning; Zhang Yi-Bo; Xing Lu; Guo Shu-Yun

doi:10.11913/PSJ.2095-0837.2022.30398

Plant Science Journal > 2022 > 40(3): 398-407. > DOI: 10.11913/PSJ.2095-0837.2022.30398 CSTR: 32231.14.PSJ.2095-0837.2022.30398

Zhang Xin-Yi, Yan Xu, Li Tie-Hua, Li Yang-Ning, Zhang Yi-Bo, Xing Lu, Guo Shu-Yun. Physiological responses of seed dormancy and germination to cold stratification in Phoebe hui Cheng ex Yang[J]. Plant Science Journal, 2022, 40(3): 398-407. DOI: 10.11913/PSJ.2095-0837.2022.30398

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Physiological responses of seed dormancy and germination to cold stratification in Phoebe hui Cheng ex Yang

Forestry College, Central South University of Forestry and Technology, Changsha 410004, China

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This work was supported by grants from the National Key Research and Development Program (2017YFD0601200), Wildlife Protection Special Project of Hunan Province (2130211), Postgraduate Scientific Research Innovation Project of Hunan Province (CX20210881), and Post-Graduates of Central South University of Forestry and Technology (cx202102002).

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Received Date: November 29, 2021
Revised Date: December 27, 2021
Available Online: October 31, 2022
Published Date: June 27, 2022

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Abstract

Abstract

Phoebe hui Cheng ex Yang is a rare and unique tree species in China, and its seeds exhibit physiological dormancy. In this study, we explored the physiological characteristics of P. hui seeds under cold stratification to reveal the response mechanism related to dormancy and germination. Specifically, we used P. hui seeds under different cold stratification time (0, 20, 40, and 60 d) to study the effects on germination percentage, nutrient levels (starch, soluble sugar, and soluble protein), peroxidase (POD) activity, and endogenous hormone levels (abscisic acid (ABA), gibberellin (GA₃), auxin (IAA), and zeatin nucleoside (ZR)), and further analyzed the correlation between seed germination and physiological indicators. Results showed that: (1) Cold stratification effectively broke P. hui seed dormancy, with stratification for 60 d showing the best effect. (2) With cold stratification, seed germination time shortened and germination percentage increased; starch content declined and soluble sugar and protein content increased; POD activity increased; ABA content decreased,GA₃ and IAA content increased, and ZR content first increased and then decreased. (3) Seed dormancy and germination of P. hui were closely related to changes in nutrient content, POD activity, and endogenous hormone content.
- Phoebe hui,
- Cold stratification,
- Seed dormancy and germination,
- Nutrients,
- Peroxidase,
- Endogenous hormone

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