Genome-wide identification and expression analysis of the <i>MAP70</i> gene family in <i>Brassica napus</i> L.

Huang Ying; Zhai Lu; Xie Ling-Li; Xu Jin-Song; Zhang Xue-Kun; Xu Ben-Bo

doi:10.11913/PSJ.2095-0837.22245

Plant Science Journal > 2023 > 41(5): 647-656. > DOI: 10.11913/PSJ.2095-0837.22245 CSTR: 32231.14.PSJ.2095-0837.22245

Huang Y，Zhai L，Xie LL，Xu JS，Zhang XK，Xu BB. Genome-wide identification and expression analysis of the MAP70 gene family in Brassica napus L.[J]. Plant Science Journal，2023，41（5）：647−656. DOI: 10.11913/PSJ.2095-0837.22245

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Genome-wide identification and expression analysis of the MAP70 gene family in Brassica napus L.

1.
Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, Hubei 434025, China
2.
College of Agriculture, Yangtze University, Jingzhou, Hubei 434025, China

Funds: This work was supported by grants from the National Key Research and Development Program of China (2017YFD0101703) and Hubei Provincial Department of Agriculture Project (E Agricultural Oil [2021] 9).

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Received Date: October 16, 2022
Revised Date: October 30, 2022
Available Online: January 09, 2023

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Abstract

Abstract

MAP70 family members were screened and identified using bioinformatics based on the genomic database of Brassica napus L., with bioinformatics analysis performed on the sequence characteristics of the identified BnaMAP70 gene family members. Expression levels of the BnaMAP70 genes were analyzed using qRT-PCR in seedling varieties with waterlogging sensitivity and tolerance. In total, 19 members of the BnaMAP70 gene family were identified, distributed on 11 chromosomes and divided into five subfamilies. The upstream elements involved in anaerobic stress and phytohormones of the BnaMAP70 promoter indicated that they may be involved in plant growth, development, and regulation of waterlogging stress. The transcription levels of genes were species-specific, plant site-specific, and time-specific. The qRT-PCR results showed that BnaMAP70-1 and BnaMAP70-4 were differentially expressed under waterlogging stress, indicating that these two genes may be regulated by waterlogging stress.
- Brassica napus,
- BnaMAP70 gene family,
- Waterlogging tolerance,
- Bioinformatics

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References (49)

References

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Genome-wide identification and expression analysis of the MAP70 gene family in Brassica napus L.