An approach to quantify the free water content in leaves of <i>Quercus acutissima</i> Carruth. with differential thermal analysis

Zhang Ping; Han Biao; Ding Ping; Zhu Jian-Jun

doi:10.11913/PSJ.2095-0837.23009

Plant Science Journal > 2023 > 41(5): 687-693. > DOI: 10.11913/PSJ.2095-0837.23009 CSTR: 32231.14.PSJ.2095-0837.23009

Zhang P，Han B，Ding P，Zhu JJ. An approach to quantify the free water content in leaves of Quercus acutissima Carruth. with differential thermal analysis[J]. Plant Science Journal，2023，41（5）：687−693. DOI: 10.11913/PSJ.2095-0837.23009

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An approach to quantify the free water content in leaves of Quercus acutissima Carruth. with differential thermal analysis

Zhang Ping^1,,
Han Biao^{2, 3},
Ding Ping³,
Zhu Jian-Jun^1, ,

1.
College of Life Sciences, Ludong University, Yantai, Shandong 264025, China
2.
Key Laboratory of State Forestry and Grassland Administration on Conservation and Utilization of Warm Temperate Zone Forest and Grass Germplasm Resources, Shandong Provincial Center of Forest and Grass Germplasm Resources, Ji’nan 250102, China
3.
Shandong Provincial Centre for Conservation and Utilization of Forest and Grass Germplasm Resources, Ji’nan 250102, China

Funds: This work was supported by grants from the National Natural Science Foundation of China (31870376) and Provincial Engineering Project of Shandong for Elite Agricultural Varieties (2019LZGC01805).

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Received Date: January 11, 2023
Revised Date: January 31, 2023
Available Online: March 13, 2023

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Abstract

Abstract

An approach was devised to quantify free water content in the leaves of sawtooth oak (Quercus acutissima Carruth.) under different states using differential thermal analysis. The freezing temperatures, peak heights of the exotherms and the areas under the exotherms were clearly observed in the time-domain scanning curve of the differential thermal analysis during cooling, while the larger areas under the exotherms were seen in the temperature-domain scanning curve of the differential thermal analysis, providing a higher sensitivity in quantifying the free water content in the samples. The boundary between free and bound water in the tissues was (−8.23 ± 0.21) MPa, close to the osmotic potential of a 2 mol/kg NaCl solution (−8.71 MPa). Freeze-killed leaves demonstrated uniform solution freezing features with sharp, narrow peaks and higher peak values (1.91 times that of living leaves) attributed to rapid temperature changes. In living leaves, the peaks were broader, and values were lower, suggesting notable delays in water phase change within cellular membranes. Thus, differential thermal analysis could effectively determine the phase change parameters of free water in plant tissues during freeze-thaw cycles and quantify the free water content in plant tissues.
- Leaves of Quercus acutissima,
- Differential thermal analysis,
- Exotherms,
- Free water,
- Bound water

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References

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An approach to quantify the free water content in leaves of Quercus acutissima Carruth. with differential thermal analysis